DOI: 10.1126/science.3187531. Recognition of a DNA Operator by the Repressor of Phage 434: A View at High Resolution

Recognition of a DNA Operator by the Repressor of Phage 434: A View at High Resolution

10.1126/science.3187531

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The repressors of temperate bacteriophages such as 434 and lambda control transcription by binding to a set of DNA operator sites. The different affinity of repressor for each of these sites ensures efficient regulation. High-resolution x-ray crystallography was used to study the DNA-binding domain of phage 434 repressor in complex with a synthetic DNA operator. The structure shows recognition of the operator by direct interactions with base pairs in the major groove, combined with the sequence-dependent ability of DNA to adopt the required conformation on binding repressor. In particular, a network of three-centered bifurcated hydrogen bonds among base pairs in the operator helps explain why 434 repressor prefers certain sites over others. These bonds, which stabilize the conformation of the bound DNA, can form only with certain sequences.

Bibliography

Aggarwal, A. K., Rodgers, D. W., Drottar, M., Ptashne, M., & Harrison, S. C. (1988). Recognition of a DNA Operator by the Repressor of Phage 434: A View at High Resolution. Science, 242(4880), 899â907.

Authors 5

Aneel K. Aggarwal (first)
David W. Rodgers (additional)
Marie Drottar (additional)
Mark Ptashne (additional)
Stephen C. Harrison (additional)

References 37 Referenced 393

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ARNOTT, S, CRC HDB BIOCH MOL BI 2: 411 (1975). / CRC HDB BIOCH MOL BI (1975)
BLUM, M, A SYSTEM FOR COLLECTION AND ONLINE INTEGRATION OF X-RAY-DIFFRACTION DATA FROM A MULTIWIRE AREA DETECTOR, JOURNAL OF APPLIED CRYSTALLOGRAPHY 20: 235 (1987). (10.1107/S0021889887086783) / JOURNAL OF APPLIED CRYSTALLOGRAPHY (1987)
BUSHMAN, F.D., ETHYLATION INTERFERENCE AND X-RAY CRYSTALLOGRAPHY IDENTIFY SIMILAR INTERACTIONS BETWEEN 434 REPRESSOR AND OPERATOR, NATURE 316: 651 (1985). (10.1038/316651a0) / NATURE (1985)
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DICKERSON, R.E., THE DNA HELIX AND HOW IT IS READ, SCIENTIFIC AMERICAN 249: 94 (1983). (10.1038/scientificamerican1283-94) / SCIENTIFIC AMERICAN (1983)
DURBIN, R.M., PROTEIN, DNA, AND VIRUS CRYSTALLOGRAPHY WITH A FOCUSED IMAGING PROPORTIONAL COUNTER, SCIENCE 232: 1127 (1986). (10.1126/science.3704639) / SCIENCE (1986)
Evans P CCP4 Suite for Protein Crystallography (s.d.).
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HOL, WGJ, ALPHA-HELIX DIPOLE AND PROPERTIES OF PROTEINS, NATURE 273: 443 (1978). (10.1038/273443a0) / NATURE (1978)
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TRONRUD, D.E., AN EFFICIENT GENERAL-PURPOSE LEAST-SQUARES REFINEMENT PROGRAM FOR MACROMOLECULAR STRUCTURES, ACTA CRYSTALLOGRAPHICA SECTION A 43: 489 (1987). (10.1107/S0108767387099124) / ACTA CRYSTALLOGRAPHICA SECTION A (1987)
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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:22 p.m.)
Indexed	1 week, 5 days ago (Aug. 19, 2025, 6:23 a.m.)
Issued	36 years, 9 months ago (Nov. 11, 1988)
Published	36 years, 9 months ago (Nov. 11, 1988)
Published Print	36 years, 9 months ago (Nov. 11, 1988)

Funders 0

None

BibTeX

@article{Aggarwal_1988, title={Recognition of a DNA Operator by the Repressor of Phage 434: A View at High Resolution}, volume={242}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3187531}, DOI={10.1126/science.3187531}, number={4880}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Aggarwal, Aneel K. and Rodgers, David W. and Drottar, Marie and Ptashne, Mark and Harrison, Stephen C.}, year={1988}, month=nov, pages={899–907} }

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