DOI: 10.1126/science.3160115. Protein-Specific Helper T-Lymphocyte Formation Initiated by Dendritic Cells

Protein-Specific Helper T-Lymphocyte Formation Initiated by Dendritic Cells

10.1126/science.3160115

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Antibody responses to hapten-polypeptide conjugates require peptide-specific helper T cells. The latter can be primed in tissue culture by providing small numbers of dendritic cells. Primed, irradiated helper T cells then induce B-cell growth and differentiation in the apparent absence of dendritic cells. Both stages of the antibody response—the induction of helper T lymphoblasts by dendritic cells and the delivery of help from T to B cell—occur in discrete cell aggregates that can be isolated by velocity sedimentation. If helper T blasts revert to smaller "memory" lymphocytes, dendritic cells again are needed to initiate the antibody response.

Authors 2

Kayo Inaba (first)
Ralph M. Steinman (additional)

References 25 Referenced 116

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AUSTYN, J.M., DENDRITIC CELLS INITIATE A 2-STAGE MECHANISM FOR LYMPHOCYTE-T PROLIFERATION, JOURNAL OF EXPERIMENTAL MEDICINE 157: 1101 (1983). (10.1084/jem.157.4.1101) / JOURNAL OF EXPERIMENTAL MEDICINE (1983)
BENACERRAF, B, HYPOTHESIS TO RELATE SPECIFICITY OF T-LYMPHOCYTES AND ACTIVITY OF I REGION-SPECIFIC IR GENES IN MACROPHAGES AND B-LYMPHOCYTES, JOURNAL OF IMMUNOLOGY 120: 1809 (1978). (10.4049/jimmunol.120.6.1809) / JOURNAL OF IMMUNOLOGY (1978)
FAUSTMAN, D.L., PREVENTION OF REJECTION OF MURINE ISLET ALLOGRAFTS BY PRETREATMENT WITH ANTI-DENDRITIC CELL ANTIBODY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 3864 (1984). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1984)
HOFFMANN, M, PERITONEAL MACROPHAGES IN IMMUNE RESPONSE TO SRBC IN-VITRO, IMMUNOLOGY 18: 791 (1970). / IMMUNOLOGY (1970)
INABA, K, DENDRITIC CELLS INDUCE LYMPHOCYTES-T TO RELEASE B-CELL-STIMULATING FACTORS BY AN INTERLEUKIN 2-DEPENDENT MECHANISM, JOURNAL OF EXPERIMENTAL MEDICINE 158: 2040 (1983). (10.1084/jem.158.6.2040) / JOURNAL OF EXPERIMENTAL MEDICINE (1983)
INABA, K, CLUSTERING OF DENDRITIC CELLS, HELPER-T, LYMPHOCYTES-T, AND HISTOCOMPATIBLE B-CELLS DURING PRIMARY ANTIBODY-RESPONSES INVITRO, JOURNAL OF EXPERIMENTAL MEDICINE 160: 858 (1984). (10.1084/jem.160.3.858) / JOURNAL OF EXPERIMENTAL MEDICINE (1984)
INABA, K, RESTING AND SENSITIZED LYMPHOCYTES-T EXHIBIT DISTINCT STIMULATORY (ANTIGEN-PRESENTING CELL) REQUIREMENTS FOR GROWTH AND LYMPHOKINE RELEASE, JOURNAL OF EXPERIMENTAL MEDICINE 160: 1717 (1984). (10.1084/jem.160.6.1717) / JOURNAL OF EXPERIMENTAL MEDICINE (1984)
INABA, K, DENDRITIC CELLS ARE CRITICAL ACCESSORY CELLS FOR THYMUS-DEPENDENT ANTIBODY-RESPONSES IN MOUSE AND IN MAN, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 80: 6041 (1983). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1983)
KATZ, D.H., CELL INTERACTIONS BETWEEN HISTOINCOMPATIBLE T AND B LYMPHOCYTES .2. FAILURE OF PHYSIOLOGIC COOPERATIVE INTERACTIONS BETWEEN T AND B LYMPHOCYTES FROM ALLOGENEIC DONOR STRAINS IN HUMORAL RESPONSE TO HAPTEN-PROTEIN CONJUGATES, JOURNAL OF EXPERIMENTAL MEDICINE 137: 1405 (1973). (10.1084/jem.137.6.1405) / JOURNAL OF EXPERIMENTAL MEDICINE (1973)
KNIGHT, C.S., PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 80: 6032 (1983). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1983)
LANGEVOORT, H.L., HISTOPHYSIOLOGY OF ANTIBODY RESPONSE .1. HISTOGENESIS OF PLASMA CELL REACTION IN RABBIT SPLEEN, LABORATORY INVESTIGATION 12: 106 (1963). / LABORATORY INVESTIGATION (1963)
LECHLER, R.I., RESTORATION OF IMMUNOGENICITY TO PASSENGER CELL-DEPLETED KIDNEY ALLOGRAFTS BY THE ADDITION OF DONOR STRAIN DENDRITIC CELLS, JOURNAL OF EXPERIMENTAL MEDICINE 155: 31 (1982). (10.1084/jem.155.1.31) / JOURNAL OF EXPERIMENTAL MEDICINE (1982)
MARRACK, P, ROLE OF H-2-LINKED GENES IN HELPER T-CELL FUNCTION .3. EXPRESSION OF IMMUNE-RESPONSE GENES FOR TRINITROPHENYL CONJUGATES OF POLY-L(TYR,GLU)-POLY-D,L-ALA--POLY-L-LYS IN B-CELLS AND MACROPHAGES, JOURNAL OF EXPERIMENTAL MEDICINE 147: 1596 (1978). (10.1084/jem.147.6.1596) / JOURNAL OF EXPERIMENTAL MEDICINE (1978)
MITCHISON, N.A., EUR J IMMUNOL 1: 18 (1971). (10.1002/eji.1830010104) / EUR J IMMUNOL (1971)
10.1126/science.158.3808.1573
NUSSENZWEIG, M.C., STUDIES OF THE CELL-SURFACE OF MOUSE DENDRITIC CELLS AND OTHER LEUKOCYTES, JOURNAL OF EXPERIMENTAL MEDICINE 154: 168 (1981). (10.1084/jem.154.1.168) / JOURNAL OF EXPERIMENTAL MEDICINE (1981)
PIERCE, C.W., IMMUNE RESPONSES IN VITRO .I. CELLULAR REQUIREMENTS FOR IMMUNE RESPONSE BY NONPRIMED AND PRIMED SPLEEN CELLS IN VITRO, JOURNAL OF EXPERIMENTAL MEDICINE 130: 345 (1969). (10.1084/jem.130.2.345) / JOURNAL OF EXPERIMENTAL MEDICINE (1969)
SPRENT, J, RESTRICTED HELPER FUNCTION OF F1 HYBRID T-CELLS POSITIVELY SELECTED TO HETEROLOGOUS ERYTHROCYTES IN IRRADIATED PARENTAL STRAIN MICE .2. EVIDENCE FOR RESTRICTIONS AFFECTING HELPER CELL INDUCTION AND T-B COLLABORATION, BOTH MAPPING TO K-END OF H-2 COMPLEX, JOURNAL OF EXPERIMENTAL MEDICINE 147: 1159 (1978). (10.1084/jem.147.4.1159) / JOURNAL OF EXPERIMENTAL MEDICINE (1978)
STEINMAN, R.M., DENDRITIC CELLS - FEATURES AND FUNCTIONS, IMMUNOLOGICAL REVIEWS 53: 127 (1980). (10.1111/j.1600-065X.1980.tb01042.x) / IMMUNOLOGICAL REVIEWS (1980)
STEINMAN, R.M., IDENTIFICATION OF A NOVEL CELL TYPE IN PERIPHERAL LYMPHOID ORGANS OF MICE, JOURNAL OF EXPERIMENTAL MEDICINE 137: 1142 (1973). (10.1084/jem.137.5.1142) / JOURNAL OF EXPERIMENTAL MEDICINE (1973)
STEINMAN, R.M., DENDRITIC CELLS ARE THE PRINCIPAL STIMULATORS OF THE PRIMARY MIXED LEUKOCYTE REACTION IN MICE, JOURNAL OF EXPERIMENTAL MEDICINE 157: 613 (1983). (10.1084/jem.157.2.613) / JOURNAL OF EXPERIMENTAL MEDICINE (1983)
STEINMAN, R.M., IDENTIFICATION OF A NOVEL CELL TYPE IN PERIPHERAL LYMPHOID ORGANS OF MICE .5. PURIFICATION OF SPLEEN DENDRITIC CELLS, NEW SURFACE MARKETS, AND MAINTENANCE INVITRO, JOURNAL OF EXPERIMENTAL MEDICINE 149: 1 (1979). (10.1084/jem.149.1.1) / JOURNAL OF EXPERIMENTAL MEDICINE (1979)
VANVOORHIS, W.C., THE PHENOTYPE OF DENDRITIC CELLS AND MACROPHAGES, FEDERATION PROCEEDINGS 42: 3114 (1983). / FEDERATION PROCEEDINGS (1983)
WITMER, M.D., THE ANATOMY OF PERIPHERAL LYMPHOID ORGANS WITH EMPHASIS ON ACCESSORY CELLS - LIGHT-MICROSCOPIC IMMUNOCYTOCHEMICAL STUDIES OF MOUSE SPLEEN, LYMPH-NODE, AND PEYERS PATCH, AMERICAN JOURNAL OF ANATOMY 170: 465 (1984). (10.1002/aja.1001700318) / AMERICAN JOURNAL OF ANATOMY (1984)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:33 p.m.)
Indexed	2 months, 2 weeks ago (June 11, 2025, 5:29 a.m.)
Issued	40 years ago (Aug. 2, 1985)
Published	40 years ago (Aug. 2, 1985)
Published Print	40 years ago (Aug. 2, 1985)

Funders 0

None

BibTeX

@article{Inaba_1985, title={Protein-Specific Helper T-Lymphocyte Formation Initiated by Dendritic Cells}, volume={229}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3160115}, DOI={10.1126/science.3160115}, number={4712}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Inaba, Kayo and Steinman, Ralph M.}, year={1985}, month=aug, pages={475–479} }

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