DOI: 10.1126/science.3158076. In Vivo Function and Membrane Binding Properties Are Correlated for <i>Escherichia coli</i> lamB Signal Peptides

In Vivo Function and Membrane Binding Properties Are Correlated for Escherichia coli lamB Signal Peptides

10.1126/science.3158076

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Wild-type and pseudorevertant signal peptides of the lamB gene product of Escherichia coli interact with lipid systems whereas a nonfunctional deletion mutant signal peptide does not. This conclusion is based on (i) interaction of synthetic signal peptides with a lipid monolayer-water surface, (ii) conformational changes induced by presence of lipid vesicles in an aqueous solution of signal peptide, and (iii) capacities of the peptides to promote vesicle aggregation. Analysis of the signal sequences and previous conformational studies suggest that these lipid interaction properties may be attributable to the tendency of the functional signal peptides to adopt α-helical conformations. Although the possibility of direct interaction between the signal peptide and membrane lipids during protein secretion is controversial, the results suggest that conformationally related amphiphilicity and consequent membrane affinity of signal sequences are important for function in vivo.

Bibliography

Briggs, M. S., Gierasch, L. M., Zlotnick, A., Lear, J. D., & DeGrado, W. F. (1985). In Vivo Function and Membrane Binding Properties Are Correlated for Escherichia coli lamB Signal Peptides. Science, 228(4703), 1096â1099.

Authors 5

Martha S. Briggs (first)
Lila M. Gierasch (additional)
Adam Zlotnick (additional)
James D. Lear (additional)
William F. DeGrado (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:07 p.m.)
Indexed	3 weeks ago (Aug. 5, 2025, 8:51 a.m.)
Issued	40 years, 2 months ago (May 31, 1985)
Published	40 years, 2 months ago (May 31, 1985)
Published Print	40 years, 2 months ago (May 31, 1985)

Funders 0

None

BibTeX

@article{Briggs_1985, title={In Vivo Function and Membrane Binding Properties Are Correlated for Escherichia coli lamB Signal Peptides}, volume={228}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3158076}, DOI={10.1126/science.3158076}, number={4703}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Briggs, Martha S. and Gierasch, Lila M. and Zlotnick, Adam and Lear, James D. and DeGrado, William F.}, year={1985}, month=may, pages={1096–1099} }

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