DOI: 10.1126/science.3059494. Human Insulin-Degrading Enzyme Shares Structural and Functional Homologies with <i>E. coli</i> Protease III

Human Insulin-Degrading Enzyme Shares Structural and Functional Homologies with E. coli Protease III

10.1126/science.3059494

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A proteinase with high affinity for insulin has been proposed to play a role in the cellular processing of this hormone. A complementary DNA (cDNA) coding for this enzyme has been isolated and sequenced. The deduced amino acid sequence of the enzyme contained the sequences of 13 peptides derived from the isolated protein. The cDNA could be transcribed in vitro to yield a synthetic RNA that in cell-free translations produced a protein that coelectrophoresed with the native proteinase and could be immunoprecipitated with monoclonal antibodies to this enzyme. The deduced sequence of this proteinase did not contain the consensus sequences for any of the known classes of proteinases (that is, metallo, cysteine, aspartic, or serine), but it did show homology to an Escherichia coli proteinase (called protease III), which also cleaves insulin and is present in the periplasmic space. Thus, these two proteins may be members of a family of proteases that are involved in intercellular peptide signaling.

Bibliography

Affholter, J. A., Fried, V. A., & Roth, R. A. (1988). Human Insulin-Degrading Enzyme Shares Structural and Functional Homologies with E. coli Protease III. Science, 242(4884), 1415â1418.

Authors 3

Joseph A. Affholter (first)
Victor A. Fried (additional)
Richard A. Roth (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 10:15 a.m.)
Indexed	1 day, 22 hours ago (Aug. 27, 2025, 11:39 a.m.)
Issued	36 years, 8 months ago (Dec. 9, 1988)
Published	36 years, 8 months ago (Dec. 9, 1988)
Published Print	36 years, 8 months ago (Dec. 9, 1988)

Funders 0

None

BibTeX

@article{Affholter_1988, title={Human Insulin-Degrading Enzyme Shares Structural and Functional Homologies with E. coli Protease III}, volume={242}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3059494}, DOI={10.1126/science.3059494}, number={4884}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Affholter, Joseph A. and Fried, Victor A. and Roth, Richard A.}, year={1988}, month=dec, pages={1415–1418} }

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