DOI: 10.1126/science.3055301. A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield

A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield

10.1126/science.3055301

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A cell-free translation system has been constructed that uses a continuous flow of the feeding buffer [including amino acids, adenosine triphosphate (ATP), and guanosine triphosphate (GTP)] through the reaction mixture and a continuous removal of a polypeptide product. Both prokaryotic ( Escherichia coli ) and eukaryotic (wheat embryos, Triticum sp. ) versions of the system have been tested. In both cases the system has proven active for long times, synthesizing polypeptides at a high constant rate for tens of hours. With the use of MS2 phage RNA or brome mosaic virus RNA 4 as templates, 100 copies of viral coat proteins per RNA were synthesized for 20 hours in the prokaryotic or eukaryotic system, respectively. With synthetic calcitonin messenger RNA, 150 to 300 copies of calcitonin polypeptide were produced per messenger RNA in both types of continuous translation systems for 40 hours.

Bibliography

Spirin, A. S., Baranov, V. I., Ryabova, L. A., Ovodov, S., & Alakhov, Y. B. (1988). A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield. Science, 242(4882), 1162â1164.

Authors 5

Alexander S. Spirin (first)
Vladimir I. Baranov (additional)
Lubov' A. Ryabova (additional)
Sergey Ovodov (additional)
Yuly B. Alakhov (additional)

References 6 Referenced 514

CHROBOCZEK, J, INCOMPLETE POLYPEPTIDES ARE FORMED INVITRO BY PREMATURE CHAIN TERMINATION, EUROPEAN JOURNAL OF BIOCHEMISTRY 149: 565 (1985). (10.1111/j.1432-1033.1985.tb08962.x) / EUROPEAN JOURNAL OF BIOCHEMISTRY (1985)
10.1093/nar/12.18.7057
10.1093/nar/12.18.7035
ROBERTS, B.E., EFFICIENT TRANSLATION OF TOBACCO MOSAIC-VIRUS RNA AND RABBIT GLOBIN-9S RNA IN A CELL-FREE SYSTEM FROM COMMERCIAL WHEAT-GERM, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 70: 2330 (1973). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1973)
SCHAGGER, H, TRICINE SODIUM DODECYL-SULFATE POLYACRYLAMIDE-GEL ELECTROPHORESIS FOR THE SEPARATION OF PROTEINS IN THE RANGE FROM 1-KDA TO 100-KDA, ANALYTICAL BIOCHEMISTRY 166: 368 (1987). (10.1016/0003-2697(87)90587-2) / ANALYTICAL BIOCHEMISTRY (1987)
SURYANARAYANA, T, AN ESSENTIAL FUNCTION OF RIBOSOMAL PROTEIN-S1 IN MESSENGER RIBONUCLEIC-ACID TRANSLATION, BIOCHEMISTRY 22: 2715 (1983). (10.1021/bi00280a020) / BIOCHEMISTRY (1983)

Dates

Type	When
Created	18 years, 11 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 10:14 a.m.)
Indexed	1 day, 20 hours ago (Sept. 4, 2025, 9:33 a.m.)
Issued	36 years, 9 months ago (Nov. 25, 1988)
Published	36 years, 9 months ago (Nov. 25, 1988)
Published Print	36 years, 9 months ago (Nov. 25, 1988)

Funders 0

None

BibTeX

@article{Spirin_1988, title={A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield}, volume={242}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3055301}, DOI={10.1126/science.3055301}, number={4882}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Spirin, Alexander S. and Baranov, Vladimir I. and Ryabova, Lubov’ A. and Ovodov, Sergey and Alakhov, Yuly B.}, year={1988}, month=nov, pages={1162–1164} }

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