DOI: 10.1126/science.3037693. A Promoter with an Internal Regulatory Domain Is Part of the Origin of Replication in BPV-1

A Promoter with an Internal Regulatory Domain Is Part of the Origin of Replication in BPV-1

10.1126/science.3037693

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Extrachromosomal elements that are stably maintained at a constant copy number through cell doublings are a good model system for the study of the regulation of DNA replication in higher eukaryotes. Previous studies have defined both cis and trans functions required for the regulated plasmid replication of the bovine papilloma virus in stably transformed cells. Here, a sequence known to be a cis-dominant element of the replication origin of the plasmid is shown to contain a promoter for transcription. Both in vitro and in vivo assays have been used to define this promoter and show that a sequence located just 3′ to the transcriptional start site is required for activity. This DNA sequence element, which has been defined through deletions, coincides with a binding site for a cellular factor and is also required for a functional origin of replication. Possible models for how a transcription factor may play a role in the regulation of DNA replication are discussed.

Bibliography

Stenlund, A., Bream, G. L., & Botchan, M. R. (1987). A Promoter with an Internal Regulatory Domain Is Part of the Origin of Replication in BPV-1. Science, 236(4809), 1666â1671.

Authors 3

Arne Stenlund (first)
Gary L. Bream (additional)
Michael R. Botchan (additional)

References 50 Referenced 57

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 9:47 a.m.)
Indexed	1 year, 7 months ago (Jan. 13, 2024, 7:07 p.m.)
Issued	38 years, 2 months ago (June 26, 1987)
Published	38 years, 2 months ago (June 26, 1987)
Published Print	38 years, 2 months ago (June 26, 1987)

Funders 0

None

BibTeX

@article{Stenlund_1987, title={A Promoter with an Internal Regulatory Domain Is Part of the Origin of Replication in BPV-1}, volume={236}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3037693}, DOI={10.1126/science.3037693}, number={4809}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Stenlund, Arne and Bream, Gary L. and Botchan, Michael R.}, year={1987}, month=jun, pages={1666–1671} }

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