DOI: 10.1126/science.3033824. Rapid Identification of Nonessential Genes of Herpes Simplex Virus Type 1 by Tn5 Mutagenesis

Rapid Identification of Nonessential Genes of Herpes Simplex Virus Type 1 by Tn5 Mutagenesis

10.1126/science.3033824

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The large genome of herpes simplex virus type 1 (HSV-1) encodes at least 80 polypeptides, the majority of which have no recognized function. A subgroup of these gene products appears to be nonessential for virus replication in cell culture, but contributes to the complex life cycle of the virus in the host. To identify such functions, a simple insertional mutagenesis method has been used for selective inactivation of individual HSV-1 genes. The bacterial transposon Tn5 was allowed to insert randomly into cloned restriction fragments representing the entire short unique (U S ) region of the HSV-1 genome. Of the 12 open reading frames that were mutagenized with Tn5, mutant derivatives of US2, US4, and US5 were recombined into the virus. These three genes proved to be nonessential for HSV-1 replication in Vero (African Green monkey kidney) cells and the US4 gene appeared to be involved in viral pathogenesis in the central nervous system of mice. This rapid mutagenesis procedure should prove useful in exploring the entire HSV-1 genome as well as the genomes of other complex animal viruses.

Bibliography

Weber, P. C., Levine, M., & Glorioso, J. C. (1987). Rapid Identification of Nonessential Genes of Herpes Simplex Virus Type 1 by Tn5 Mutagenesis. Science, 236(4801), 576â579.

Authors 3

Peter C. Weber (first)
Myron Levine (additional)
Joseph C. Glorioso (additional)

References 19 Referenced 102

BERG, D.E., THE PROKARYOTIC TRANSPOSABLE ELEMENT TN5, BIO-TECHNOLOGY 1: 417 (1983). (10.1038/nbt0783-417) / BIO-TECHNOLOGY (1983)
DEBRUIJN, F.J., THE USE OF TRANSPOSON TN5 MUTAGENESIS IN THE RAPID GENERATION OF CORRELATED PHYSICAL AND GENETIC MAPS OF DNA SEGMENTS CLONED INTO MULTICOPY PLASMIDS - A REVIEW, GENE 27: 131 (1984). (10.1016/0378-1119(84)90135-5) / GENE (1984)
FIELD, H.J., PATHOGENICITY OF THYMIDINE KINASE-DEFICIENT MUTANTS OF HERPES-SIMPLEX VIRUS IN MICE, JOURNAL OF HYGIENE 81: 267 (1978). (10.1017/S0022172400025109) / JOURNAL OF HYGIENE (1978)
FRAME, M.C., NOVEL HERPES-SIMPLEX VIRUS TYPE-1 GLYCOPROTEINS IDENTIFIED BY ANTISERUM AGAINST A SYNTHETIC OLIGOPEPTIDE FROM THE PREDICTED PRODUCT OF GENE US4, JOURNAL OF GENERAL VIROLOGY 67: 745 (1986). (10.1099/0022-1317-67-4-745) / JOURNAL OF GENERAL VIROLOGY (1986)
FRAME, M.C., THE 10K VIRION PHOSPHOPROTEIN ENCODED BY GENE US9 FROM HERPES-SIMPLEX VIRUS TYPE-1, VIROLOGY 150: 321 (1986). (10.1016/0042-6822(86)90297-7) / VIROLOGY (1986)
HOMA, F.L., TRANSCRIPTIONAL CONTROL SIGNALS OF A HERPES-SIMPLEX VIRUS TYPE-1 LATE (GAMMA-2) GENE LIE WITHIN BASES -34 TO +124 RELATIVE TO THE 5' TERMINUS OF THE MESSENGER-RNA, MOLECULAR AND CELLULAR BIOLOGY 6: 3652 (1986). / MOLECULAR AND CELLULAR BIOLOGY (1986)
JENKINS, F.J., APPLICATION OF THE MINI-MU-PHAGE FOR TARGET-SEQUENCE-SPECIFIC INSERTIONAL MUTAGENESIS OF THE HERPES-SIMPLEX VIRUS GENOME, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 4773 (1985). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1985)
JOHNSON, P.A., THE PRODUCT OF GENE US11 OF HERPES-SIMPLEX VIRUS TYPE-1 IS EXPRESSED AS A TRUE LATE GENE, JOURNAL OF GENERAL VIROLOGY 67: 871 (1986). (10.1099/0022-1317-67-5-871) / JOURNAL OF GENERAL VIROLOGY (1986)
KUMEL, G, PASSIVE IMMUNE PROTECTION BY HERPES-SIMPLEX VIRUS-SPECIFIC MONOCLONAL-ANTIBODIES AND MONOCLONAL ANTIBODY-RESISTANT MUTANTS ALTERED IN PATHOGENICITY, JOURNAL OF VIROLOGY 56: 930 (1985). (10.1128/jvi.56.3.930-937.1985) / JOURNAL OF VIROLOGY (1985)
LEE, GTY, LOCATION OF THE STRUCTURAL GENES FOR GLYCOPROTEINS GD AND GE AND FOR OTHER POLYPEPTIDES IN THE S COMPONENT OF HERPES-SIMPLEX VIRUS TYPE-1 DNA, JOURNAL OF VIROLOGY 43: 41 (1982). (10.1128/jvi.43.1.41-49.1982) / JOURNAL OF VIROLOGY (1982)
LONGNECKER, R, GENERATION OF AN INVERTING HERPES-SIMPLEX VIRUS-1 MUTANT LACKING THE L-S JUNCTION A-SEQUENCES, AN ORIGIN OF DNA-SYNTHESIS, AND SEVERAL GENES INCLUDING THOSE SPECIFYING GLYCOPROTEIN-E AND THE ALPHA-47 GENE, JOURNAL OF VIROLOGY 58: 583 (1986). (10.1128/jvi.58.2.583-591.1986) / JOURNAL OF VIROLOGY (1986)
MCGEOCH, D.J., SEQUENCE DETERMINATION AND GENETIC CONTENT OF THE SHORT UNIQUE REGION IN THE GENOME OF HERPES-SIMPLEX VIRUS TYPE-1, JOURNAL OF MOLECULAR BIOLOGY 181: 1 (1985). (10.1016/0022-2836(85)90320-1) / JOURNAL OF MOLECULAR BIOLOGY (1985)
MCGEOCH, D.J., ALPHAHERPESVIRUSES POSSESS A GENE HOMOLOGOUS TO THE PROTEIN-KINASE GENE FAMILY OF EUKARYOTES AND RETROVIRUSES, NUCLEIC ACIDS RESEARCH 14: 1765 (1986). (10.1093/nar/14.4.1765) / NUCLEIC ACIDS RESEARCH (1986)
MURCHIE, M.J., DNA-SEQUENCE ANALYSIS OF AN IMMEDIATE-EARLY GENE REGION OF THE HERPES-SIMPLEX VIRUS TYPE-1 GENOME (MAP COORDINATES 0.950 TO 0.978), JOURNAL OF GENERAL VIROLOGY 62: 1 (1982). (10.1099/0022-1317-62-1-1) / JOURNAL OF GENERAL VIROLOGY (1982)
POST, L.E., GENERALIZED TECHNIQUE FOR DELETION OF SPECIFIC GENES IN LARGE GENOMES - ALPHA-GENE-22 OF HERPES-SIMPLEX VIRUS-1 IS NOT ESSENTIAL FOR GROWTH, CELL 25: 227 (1981). (10.1016/0092-8674(81)90247-6) / CELL (1981)
RIXON, F.J., DETAILED ANALYSIS OF THE MESSENGER-RNAS MAPPING IN THE SHORT UNIQUE REGION OF HERPES-SIMPLEX VIRUS TYPE-1, NUCLEIC ACIDS RESEARCH 13: 953 (1985). (10.1093/nar/13.3.953) / NUCLEIC ACIDS RESEARCH (1985)
SEARS, A.E., HERPES-SIMPLEX VIRUS-1 MUTANT DELETED IN THE ALPHA-22 GENE - GROWTH AND GENE-EXPRESSION IN PERMISSIVE AND RESTRICTIVE CELLS AND ESTABLISHMENT OF LATENCY IN MICE, JOURNAL OF VIROLOGY 55: 338 (1985). (10.1128/jvi.55.2.338-346.1985) / JOURNAL OF VIROLOGY (1985)
10.1126/science.6289440
WHITLEY, R.J., HERPESVIRUSES 3: 1 (1985). / HERPESVIRUSES (1985)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 9:47 a.m.)
Indexed	1 year ago (Aug. 11, 2024, 3:12 p.m.)
Issued	38 years, 3 months ago (May 1, 1987)
Published	38 years, 3 months ago (May 1, 1987)
Published Print	38 years, 3 months ago (May 1, 1987)

Funders 0

None

BibTeX

@article{Weber_1987, title={Rapid Identification of Nonessential Genes of Herpes Simplex Virus Type 1 by Tn5 Mutagenesis}, volume={236}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3033824}, DOI={10.1126/science.3033824}, number={4801}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Weber, Peter C. and Levine, Myron and Glorioso, Joseph C.}, year={1987}, month=may, pages={576–579} }

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