DOI: 10.1126/science.3022376. Two Avirulent Herpes Simplex Viruses Generate Lethal Recombinants in Vivo

Two Avirulent Herpes Simplex Viruses Generate Lethal Recombinants in Vivo

10.1126/science.3022376

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

While it is widely appreciated that infection with a virulent virus can produce disease in an animal, the ability of a mixture of avirulent viruses to produce disease by means of complementation or recombination in vivo has not been established. In this study, two weakly neuroinvasive herpes simplex virus type 1 (HSV-1) strains were simultaneously inoculated onto the footpads of mice. Many (62%) of the animals that received a 1:1 mixture of the viruses died, whereas the animals that received a similar or 100-fold higher dose of each agent alone survived. Of fourteen viruses isolated from the brains of ten mice that died after receiving the mixture of the two weakly neuroinvasive viruses, eleven were recombinants; three of these recombinants were lethal when reapplied to the footpads of mice. These results show that two avirulent HSV-1 variants may interact in vivo to produce virulent recombinants and a lethal infection. They also suggest that different genetic lesions account for the weakly neuroinvasive character of the HSV-1 strains ANG and KOS after footpad inoculation.

Bibliography

Javier, R. T., Sedarati, F., & Stevens, J. G. (1986). Two Avirulent Herpes Simplex Viruses Generate Lethal Recombinants in Vivo. Science, 234(4777), 746â748.

Authors 3

R. T. Javier (first)
F. Sedarati (additional)
J. G. Stevens (additional)

References 21 Referenced 103

DIX, R.D., COMPARATIVE NEUROVIRULENCE OF HERPES-SIMPLEX VIRUS TYPE-1 STRAINS AFTER PERIPHERAL OR INTRACEREBRAL INOCULATION OF BALB/C MICE, INFECTION AND IMMUNITY 40: 103 (1983). (10.1128/iai.40.1.103-112.1983) / INFECTION AND IMMUNITY (1983)
HILL, T.J., HERPES VIRUSES: 175 (1982). / HERPES VIRUSES (1982)
HINSHAW, V.S., GENETIC REASSORTMENT OF INFLUENZA A-VIRUSES IN THE INTESTINAL-TRACT OF DUCKS, VIROLOGY 102: 412 (1980). (10.1016/0042-6822(80)90108-7) / VIROLOGY (1980)
IZUMI K unpublished data.
KAERNER, H.C., GENETIC-VARIABILITY OF HERPES-SIMPLEX VIRUS - DEVELOPMENT OF A PATHOGENIC VARIANT DURING PASSAGING OF A NON-PATHOGENIC HERPES-SIMPLEX VIRUS TYPE-1 VIRUS-STRAIN IN MOUSE-BRAIN, JOURNAL OF VIROLOGY 46: 83 (1983). (10.1128/jvi.46.1.83-93.1983) / JOURNAL OF VIROLOGY (1983)
KIRCHNER, H, MONOGRAPHS VIROLOGY: 1 (1982). / MONOGRAPHS VIROLOGY (1982)
KUMEL, G, EXPERIMENTAL-INFECTION OF INBRED MICE WITH HERPES-SIMPLEX VIRUS .5. INVESTIGATIONS WITH A VIRUS-STRAIN NON-LETHAL AFTER PERIPHERAL INFECTION, JOURNAL OF GENERAL VIROLOGY 63: 315 (1982). (10.1099/0022-1317-63-2-315) / JOURNAL OF GENERAL VIROLOGY (1982)
Maniatis T. Molecular Cloning: A Laboratory Manual (1982).
10.1126/science.93.2424.574
RAWLS, W.E., VIROLOGY: 527 (1985). / VIROLOGY (1985)
REED, L.J., AMERICAN JOURNAL OF HYGIENE 27: 493 (1938). / AMERICAN JOURNAL OF HYGIENE (1938)
RIGBY, PWJ, LABELING DEOXYRIBONUCLEIC-ACID TO HIGH SPECIFIC ACTIVITY INVITRO BY NICK TRANSLATION WITH DNA-POLYMERASE I, JOURNAL OF MOLECULAR BIOLOGY 113: 237 (1977). (10.1016/0022-2836(77)90052-3) / JOURNAL OF MOLECULAR BIOLOGY (1977)
10.1016/S0022-2836(75)80083-0
SUGIYAMA, K, ANALYSES OF THE GENOMES OF BLUETONGUE VIRUSES RECOVERED IN THE UNITED-STATES .1. OLIGONUCLEOTIDE FINGERPRINT STUDIES THAT INDICATE THE EXISTENCE OF NATURALLY-OCCURRING REASSORTANT BTV ISOLATES, VIROLOGY 114: 210 (1981). (10.1016/0042-6822(81)90266-X) / VIROLOGY (1981)
THOMPSON, R.L., RESCUE OF A HERPES-SIMPLEX VIRUS TYPE-1 NEUROVIRULENCE FUNCTION WITH A CLONED DNA FRAGMENT, JOURNAL OF VIROLOGY 55: 504 (1985). (10.1128/jvi.55.2.504-508.1985) / JOURNAL OF VIROLOGY (1985)
THOMPSON, R.L., FUNCTIONAL AND MOLECULAR ANALYSES OF THE AVIRULENT WILD-TYPE HERPES-SIMPLEX VIRUS TYPE-1 STRAIN KOS, JOURNAL OF VIROLOGY 58: 203 (1986). (10.1128/jvi.58.1.203-211.1986) / JOURNAL OF VIROLOGY (1986)
THOMPSON, R.L., BIOLOGICAL CHARACTERIZATION OF A HERPES-SIMPLEX VIRUS INTERTYPIC RECOMBINANT WHICH IS COMPLETELY AND SPECIFICALLY NON-NEUROVIRULENT, VIROLOGY 131: 171 (1983). (10.1016/0042-6822(83)90543-3) / VIROLOGY (1983)
THOMPSON, R.L., PHYSICAL LOCATION OF A HERPES-SIMPLEX VIRUS TYPE-1 GENE FUNCTION(S) SPECIFICALLY ASSOCIATED WITH A 10 MILLION-FOLD INCREASE IN HSV NEUROVIRULENCE, VIROLOGY 131: 180 (1983). (10.1016/0042-6822(83)90544-5) / VIROLOGY (1983)
WAHL, G.M., EFFICIENT TRANSFER OF LARGE DNA FRAGMENTS FROM AGAROSE GELS TO DIAZOBENZYLOXYMETHYL-PAPER AND RAPID HYBRIDIZATION BY USING DEXTRAN SULFATE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 3683 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
WALBOOMERS, JMM, NEW METHOD FOR ISOLATION OF HERPES-SIMPLEX VIRUS TYPE-2 DNA, VIROLOGY 74: 256 (1976). (10.1016/0042-6822(76)90151-3) / VIROLOGY (1976)
WENSKE, E.A., GENETIC REASSORTMENT OF MAMMALIAN REOVIRUSES IN MICE, JOURNAL OF VIROLOGY 56: 613 (1985). (10.1128/jvi.56.2.613-616.1985) / JOURNAL OF VIROLOGY (1985)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 10:36 a.m.)
Indexed	4 months, 1 week ago (April 8, 2025, 10:19 p.m.)
Issued	38 years, 9 months ago (Nov. 7, 1986)
Published	38 years, 9 months ago (Nov. 7, 1986)
Published Print	38 years, 9 months ago (Nov. 7, 1986)

Funders 0

None

BibTeX

@article{Javier_1986, title={Two Avirulent Herpes Simplex Viruses Generate Lethal Recombinants in Vivo}, volume={234}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3022376}, DOI={10.1126/science.3022376}, number={4777}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Javier, R. T. and Sedarati, F. and Stevens, J. G.}, year={1986}, month=nov, pages={746–748} }

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