DOI: 10.1126/science.3006254. Receptor-Coupled Activation of Phosphoinositide-Specific Phospholipase C by an N Protein

Receptor-Coupled Activation of Phosphoinositide-Specific Phospholipase C by an N Protein

10.1126/science.3006254

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Cleavage of phosphatidylinositol 4,5-bisphosphate by phospholipase C results in the production of two important second messengers: inositol-1,4,5-trisphosphate and 1,2-diacylglycerol. Although several receptors promote this cleavage, the molecular details of phospholipase C activation have remained unresolved. In this study, occupancy of a Ca 2+ -mobilizing receptor, the oligopeptide chemoattractant receptor on human polymorphonuclear leukocyte plasma membranes, was found to lead to the activation of a guanine nucleotide regulatory (N) protein by guanosine 5′-triphosphate. The activated N protein then stimulated a polyphosphoinositide-specific phospholipase C by reducing the Ca 2+ requirement for expression of this activity from superphysiological to normal intracellular concentrations. Therefore, the N protein-mediated activation of phospholipase C may be a key step in the pathway of cellular activation by chemoattractants and certain other hormones.

Authors 3

Charles D. Smith (first)
C. Christine Cox (additional)
Ralph Snyderman (additional)

References 34 Referenced 245

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 10:25 a.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:38 p.m.)
Issued	39 years, 4 months ago (April 4, 1986)
Published	39 years, 4 months ago (April 4, 1986)
Published Print	39 years, 4 months ago (April 4, 1986)

Funders 0

None

BibTeX

@article{Smith_1986, title={Receptor-Coupled Activation of Phosphoinositide-Specific Phospholipase C by an N Protein}, volume={232}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3006254}, DOI={10.1126/science.3006254}, number={4746}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Smith, Charles D. and Cox, C. Christine and Snyderman, Ralph}, year={1986}, month=apr, pages={97–100} }

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