DOI: 10.1126/science.3001934. Binding of HTLV-III/LAV to T4 <sup>+</sup> T Cells by a Complex of the 110K Viral Protein and the T4 Molecule

Binding of HTLV-III/LAV to T4 + T Cells by a Complex of the 110K Viral Protein and the T4 Molecule

10.1126/science.3001934

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Human T-lymphotropic virus type III (HTLV-III) or lymphadenopathy-associated virus (LAV) is tropic for human T cells with the helper-inducer phenotype, as defined by reactivity with monoclonal antibodies specific for the T4 molecule. Treatment of T4 + T cells with monoclonal antibodies to T4 antigen blocks HTLV-III/LAV binding, syncytia formation, and infectivity. Thus, it has been inferred that the T4 molecule itself is a virus receptor. In the present studies, the surfaces of T4 + T cells were labeled radioactively, and then the cells were exposed to virus. After the cells were lysed, HTLV-III/LAV antibodies were found to precipitate a surface protein with a molecular weight of 58,000 (58K). By blocking and absorption experiments, this 58K protein was identified as the T4 molecule. No cell-surface structures other than the T4 molecule were involved in the antibody-antigen complex formation. Two monoclonal antibodies, each reactive with a separate epitope of the T4 molecule, were tested for their binding capacities in the presence of HTLV-III/LAV. When HTLV-III/LAV was bound to T4 + T cells, the virus blocked the binding of one of the monoclonal antibodies, T4A (OKT4A), but not of the other, T4 (OKT4). When HTLV-III/LAV was internally radiolabeled and bound to T4 + T cells which were then lysed, a viral glycoprotein of 110K (gp110) coprecipitated with the T4 molecule. The binding of gp110 to the T4 molecule may thus be a major factor in HTLV-III/LAV tropism and may prove useful in developing therapeutic or preventive measures for the acquired immune deficiency syndrome.

Bibliography

McDougal, J. S., Kennedy, M. S., Sligh, J. M., Cort, S. P., Mawle, A., & Nicholson, J. K. A. (1986). Binding of HTLV-III/LAV to T4 + T Cells by a Complex of the 110K Viral Protein and the T4 Molecule. Science, 231(4736), 382â385.

Authors 6

J. S. McDougal (first)
M. S. Kennedy (additional)
J. M. Sligh (additional)
S. P. Cort (additional)
A. Mawle (additional)
J. K. A. Nicholson (additional)

References 34 Referenced 799

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 10:17 a.m.)
Indexed	4 months ago (April 18, 2025, 11:24 a.m.)
Issued	39 years, 6 months ago (Jan. 24, 1986)
Published	39 years, 6 months ago (Jan. 24, 1986)
Published Print	39 years, 6 months ago (Jan. 24, 1986)

Funders 0

None

BibTeX

@article{McDougal_1986, title={Binding of HTLV-III/LAV to T4 + T Cells by a Complex of the 110K Viral Protein and the T4 Molecule}, volume={231}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.3001934}, DOI={10.1126/science.3001934}, number={4736}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={McDougal, J. S. and Kennedy, M. S. and Sligh, J. M. and Cort, S. P. and Mawle, A. and Nicholson, J. K. A.}, year={1986}, month=jan, pages={382–385} }

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