DOI: 10.1126/science.2999983. Promoter Region of the Human Harvey <i>ras</i> Proto-Oncogene: Similarity to the EGF Receptor Proto-Oncogene Promoter

Promoter Region of the Human Harvey ras Proto-Oncogene: Similarity to the EGF Receptor Proto-Oncogene Promoter

10.1126/science.2999983

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Regulation of transcription of members of the ras gene family undoubtably plays an important role in controlling cellular growth. Examination of this level of regulation requires identification of the promoter regions of the ras proto-oncogenes. Four major transcriptional start sites were detected in the human Harvey ras 1 proto-oncogene. The promoter region contains neither a TATA box nor a CAAT box in their characteristic upstream positions, has an extremely high G+C content (80 percent), and contains multiple GC boxes including seven CCGCCC repeats and three repeats of the inverted complement, GGGCGG. This region has strong promoter activity when placed upstream from the chloramphenicol acetyl transferase gene and transfected into monkey CV1 cells. In these ways the Harvey ras 1 proto-oncogene promoter resembles the promoter of the gene encoding the epidermal growth factor (EGF) receptor. The similarity between the two proto-oncogene promoters may be relevant to the mechanism by which the expression of such "growth control" genes is regulated.

Bibliography

Ishii, S., Merlino, G. T., & Pastan, I. (1985). Promoter Region of the Human Harvey ras Proto-Oncogene: Similarity to the EGF Receptor Proto-Oncogene Promoter. Science, 230(4732), 1378â1381.

Authors 3

Shunsuke Ishii (first)
Glenn T. Merlino (additional)
Ira Pastan (additional)

References 38 Referenced 151

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 10:28 a.m.)
Indexed	1 year, 7 months ago (Jan. 13, 2024, 7:07 p.m.)
Issued	39 years, 8 months ago (Dec. 20, 1985)
Published	39 years, 8 months ago (Dec. 20, 1985)
Published Print	39 years, 8 months ago (Dec. 20, 1985)

Funders 0

None

BibTeX

@article{Ishii_1985, title={Promoter Region of the Human Harvey ras Proto-Oncogene: Similarity to the EGF Receptor Proto-Oncogene Promoter}, volume={230}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2999983}, DOI={10.1126/science.2999983}, number={4732}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Ishii, Shunsuke and Merlino, Glenn T. and Pastan, Ira}, year={1985}, month=dec, pages={1378–1381} }

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