DOI: 10.1126/science.2983423. Measuring Gene Expression with Light

10.1126/science.2983423

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Light is produced by recombinant Escherichia coli that contain lux genes cloned from the marine bacterium Vibrio fischeri . The bioluminescence phenotype requires genes for regulatory and biochemical functions, the latter encoded by five lux genes contained in a single operon. These lux genes were disconnected from their native promoter and inserted into the transposon mini-Mu. The resulting transposon, mini-Mu lux , could induce mutations by insertional inactivation of a target gene, and the lux DNA was oriented to align target gene transcription with that of the lux genes. Genes in Escherichia coli and Vibrio parahaemolyticus were mutagenized, and mutants containing transposon-generated lux gene fusions produced light as a function of target gene transcription. Light production offers a simple, sensitive, in vivo indicator of gene expression.

Bibliography

Engebrecht, J., Simon, M., & Silverman, M. (1985). Measuring Gene Expression with Light. Science, 227(4692), 1345â1347.

Authors 3

JoAnne Engebrecht (first)
Melvin Simon (additional)
Michael Silverman (additional)

References 10 Referenced 123

Bassford, P., The Operon: 245 (1978). / The Operon (1978)
BELAS, R, TRANSPOSON MUTAGENESIS OF MARINE VIBRIO SPP, JOURNAL OF BACTERIOLOGY 158: 890 (1984). (10.1128/jb.158.3.890-896.1984) / JOURNAL OF BACTERIOLOGY (1984)
10.1126/science.10636771
10.1073/pnas.76.9.4530
DECASTILHO, B.A., J BACTERIOL 158: 488 (1984). (10.1128/jb.158.2.488-495.1984) / J BACTERIOL (1984)
10.1016/0092-8674(83)90063-6
ENGEBRECHT, J, IDENTIFICATION OF GENES AND GENE-PRODUCTS NECESSARY FOR BACTERIAL BIOLUMINESCENCE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 4154 (1984). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1984)
ENGEBRECHT J unpublished data.
KLECKNER, N, GENETIC ENGINEERING INVIVO USING TRANSLOCATABLE DRUG-RESISTANCE ELEMENTS - NEW METHODS IN BACTERIAL GENETICS, JOURNAL OF MOLECULAR BIOLOGY 116: 125 (1977). (10.1016/0022-2836(77)90123-1) / JOURNAL OF MOLECULAR BIOLOGY (1977)
ZIEGLER, M.M., BIOCHEMISTRY OF BACTERIAL BIOLUMINESCENCE, CURRENT TOPICS IN BIOENERGETICS 12: 65 (1981). (10.1016/B978-0-12-152512-5.50008-7) / CURRENT TOPICS IN BIOENERGETICS (1981)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 9:50 a.m.)
Indexed	4 months ago (April 30, 2025, 4:25 a.m.)
Issued	40 years, 5 months ago (March 15, 1985)
Published	40 years, 5 months ago (March 15, 1985)
Published Print	40 years, 5 months ago (March 15, 1985)

Funders 0

None

BibTeX

@article{Engebrecht_1985, title={Measuring Gene Expression with Light}, volume={227}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2983423}, DOI={10.1126/science.2983423}, number={4692}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Engebrecht, JoAnne and Simon, Melvin and Silverman, Michael}, year={1985}, month=mar, pages={1345–1347} }

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