DOI: 10.1126/science.2950591. Construction of a General Human Chromosome Jumping Library, with Application to Cystic Fibrosis

Construction of a General Human Chromosome Jumping Library, with Application to Cystic Fibrosis

10.1126/science.2950591

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

In many genetic disorders, the responsible gene and its protein product are unknown. The technique known as "reverse genetics," in which chromosomal map positions and genetically linked DNA markers are used to identify and clone such genes, is complicated by the fact that the molecular distances from the closest DNA markers to the gene itself are often too large to traverse by standard cloning techniques. To address this situation, a general human chromosome jumping library was constructed that allows the cloning of DNA sequences approximately 100 kilobases away from any starting point in genomic DNA. As an illustration of its usefulness, this library was searched for a jumping clone, starting at the met oncogene, which is a marker tightly linked to the cystic fibrosis gene that is located on human chromosome 7. Mapping of the new genomic fragment by pulsed field gel electrophoresis confirmed that it resides on chromosome 7 within 240 kilobases downstream of the met gene. The use of chromosome jumping should now be applicable to any genetic locus for which a closely linked DNA marker is available.

Bibliography

Collins, F. S., Drumm, M. L., Cole, J. L., Lockwood, W. K., Vande Woude, G. F., & Iannuzzi, M. C. (1987). Construction of a General Human Chromosome Jumping Library, with Application to Cystic Fibrosis. Science, 235(4792), 1046â1049.

Authors 6

Francis S. Collins (first)
Mitchell L. Drumm (additional)
Jeffery L. Cole (additional)
Wendy K. Lockwood (additional)
George F. Vande Woude (additional)
Michael C. Iannuzzi (additional)

References 39 Referenced 144

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 10:03 a.m.)
Indexed	1 month ago (July 22, 2025, 7:15 a.m.)
Issued	38 years, 6 months ago (Feb. 27, 1987)
Published	38 years, 6 months ago (Feb. 27, 1987)
Published Print	38 years, 6 months ago (Feb. 27, 1987)

Funders 0

None

BibTeX

@article{Collins_1987, title={Construction of a General Human Chromosome Jumping Library, with Application to Cystic Fibrosis}, volume={235}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2950591}, DOI={10.1126/science.2950591}, number={4792}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Collins, Francis S. and Drumm, Mitchell L. and Cole, Jeffery L. and Lockwood, Wendy K. and Vande Woude, George F. and Iannuzzi, Michael C.}, year={1987}, month=feb, pages={1046–1049} }

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