DOI: 10.1126/science.2948278. A Subset of Yeast snRNA's Contains Functional Binding Sites for the Highly Conserved Sm Antigen

A Subset of Yeast snRNA's Contains Functional Binding Sites for the Highly Conserved Sm Antigen

10.1126/science.2948278

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Autoimmune sera of the Sm specificity react with the major class of small nuclear RNA (snRNA)-containing ribonucleoprotein particles (snRNP's) from organisms as evolutionarily divergent as insects and dinoflagellates but have been reported not to recognize snRNP's from yeast. The Sm antigen is thought to bind to a conserved snRNA motif that includes the sequence A(U 3-6 )G. The hypothesis was tested that yeast also contains functional analogs of Sm snRNA's, but that the Sm binding site in the RNA is more strictly conserved than the Sm antigenic determinant. After microinjection of labeled yeast snRNA's into Xenopus eggs or oocytes, two snRNA's from Saccharomyces cerevisiae become strongly immunoprecipitable with human auto-antibodies known as anti-Sm. These each contain the sequence A(U 5-6 )G, are essential for viability, and are constituents of the spliceosome. At least six other yeast snRNA's do not become immunoprecipitable and lack this sequence; these non-Sm snRNA's are all dispensable.

Bibliography

Riedel, N., Wolin, S., & Guthrie, C. (1987). A Subset of Yeast snRNAâs Contains Functional Binding Sites for the Highly Conserved Sm Antigen. Science, 235(4786), 328â331.

Authors 3

Nora Riedel (first)
Sandra Wolin (additional)
Christine Guthrie (additional)

References 37 Referenced 50

ARES, M, U2 RNA FROM YEAST IS UNEXPECTEDLY LARGE AND CONTAINS HOMOLOGY TO VERTEBRATE U4, U5, AND U6 SMALL NUCLEAR RNAS, CELL 47: 49 (1986). (10.1016/0092-8674(86)90365-X) / CELL (1986)
BLACK, D.L., U2 AS WELL AS U1 SMALL NUCLEAR RIBONUCLEOPROTEINS ARE INVOLVED IN PRE-MESSENGER RNA SPLICING, CELL 42: 737 (1985). (10.1016/0092-8674(85)90270-3) / CELL (1985)
BLACK, D.L., PRE-MESSENGER-RNA SPLICING INVITRO REQUIRES INTACT U4/U6 SMALL NUCLEAR RIBONUCLEOPROTEIN, CELL 46: 697 (1986). (10.1016/0092-8674(86)90345-4) / CELL (1986)
BRANLANT, C, U2 RNA SHARES A STRUCTURAL DOMAIN WITH U1, U4, AND U5 RNAS, EMBO JOURNAL 1: 1259 (1982). (10.1002/j.1460-2075.1982.tb00022.x) / EMBO JOURNAL (1982)
BRINGMANN, P, EVIDENCE FOR THE EXISTENCE OF SNRNAS U4 AND U6 IN A SINGLE RIBONUCLEOPROTEIN COMPLEX AND FOR THEIR ASSOCIATION BY INTERMOLECULAR BASE-PAIRING, EMBO JOURNAL 3: 1357 (1984). (10.1002/j.1460-2075.1984.tb01977.x) / EMBO JOURNAL (1984)
BROWN, D.T., STRUCTURE OF THE SEA-URCHIN U1 RNA REPEAT, NUCLEIC ACIDS RESEARCH 13: 537 (1985). (10.1093/nar/13.2.537) / NUCLEIC ACIDS RESEARCH (1985)
10.1126/science.2933810
CHENG, S.C., FRACTIONATION AND CHARACTERIZATION OF A YEAST MESSENGER-RNA SPLICING EXTRACT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 2387 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
DEROBERTIS, E.M., INTRACELLULAR-TRANSPORT OF MICRO-INJECTED 5S AND SMALL NUCLEAR RNAS, NATURE 295: 572 (1982). (10.1038/295572a0) / NATURE (1982)
FORBES, D.J., SMALL NUCLEAR-RNA TRANSCRIPTION AND RIBONUCLEOPROTEIN ASSEMBLY IN EARLY XENOPUS DEVELOPMENT, JOURNAL OF CELL BIOLOGY 97: 62 (1983). (10.1083/jcb.97.1.62) / JOURNAL OF CELL BIOLOGY (1983)
GUTHRIE, C, FINDING FUNCTIONS FOR SMALL NUCLEAR RNAS IN YEAST, TRENDS IN BIOCHEMICAL SCIENCES 11: 430 (1986). (10.1016/0968-0004(86)90179-9) / TRENDS IN BIOCHEMICAL SCIENCES (1986)
GUTHRIE, C, YEAST CELL BIOL: 301 (1986). / YEAST CELL BIOL (1986)
HASHIMOTO, C, U4 AND U6 RNAS COEXIST IN A SINGLE SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE, NUCLEIC ACIDS RESEARCH 12: 3283 (1984). (10.1093/nar/12.7.3283) / NUCLEIC ACIDS RESEARCH (1984)
KRAINER, A.R., MULTIPLE FACTORS INCLUDING THE SMALL NUCLEAR RIBONUCLEOPROTEINS U1 AND U2 ARE NECESSARY FOR PRE-MESSENGER RNA SPLICING INVITRO, CELL 42: 725 (1985). (10.1016/0092-8674(85)90269-7) / CELL (1985)
KRAMER, A, THE 5' TERMINUS OF THE RNA MOIETY OF U1 SMALL NUCLEAR RIBONUCLEOPROTEIN-PARTICLES IS REQUIRED FOR THE SPLICING OF MESSENGER-RNA PRECURSORS, CELL 38: 299 (1984). (10.1016/0092-8674(84)90551-8) / CELL (1984)
LERNER, M.R., ARE SNRNPS INVOLVED IN SPLICING, NATURE 283: 220 (1980). (10.1038/283220a0) / NATURE (1980)
LERNER, M.R., ANTIBODIES TO SMALL NUCLEAR RNAS COMPLEXED WITH PROTEINS ARE PRODUCED BY PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 76: 5495 (1979). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1979)
LIAUTARD, J.P., STRUCTURAL ORGANIZATION OF RIBONUCLEOPROTEINS CONTAINING SMALL NUCLEAR RNAS FROM HELA-CELLS - PROTEINS INTERACT CLOSELY WITH A SIMILAR STRUCTURAL DOMAIN OF U1, U2, U4 AND U5 SMALL NUCLEAR RNAS, JOURNAL OF MOLECULAR BIOLOGY 162: 623 (1982). (10.1016/0022-2836(82)90392-8) / JOURNAL OF MOLECULAR BIOLOGY (1982)
MATTAJ, I.W., NUCLEAR SEGREGATION OF U2 SNRNA REQUIRES BINDING OF SPECIFIC SNRNP PROTEINS, CELL 40: 111 (1985). (10.1016/0092-8674(85)90314-9) / CELL (1985)
MOUNT, S.M., SEQUENCE OF U1 RNA FROM DROSOPHILA-MELANOGASTER - IMPLICATIONS FOR U1 SECONDARY STRUCTURE AND POSSIBLE INVOLVEMENT IN SPLICING, NUCLEIC ACIDS RESEARCH 9: 6351 (1981). (10.1093/nar/9.23.6351) / NUCLEIC ACIDS RESEARCH (1981)
PADGETT, R.A., SPLICING OF MESSENGER-RNA PRECURSORS IS INHIBITED BY ANTISERA TO SMALL NUCLEAR RIBONUCLEOPROTEIN, CELL 35: 101 (1983). (10.1016/0092-8674(83)90212-X) / CELL (1983)
PARKER R unpublished data.
PATTERSON B unpublished data.
PETTERSSON, I, THE STRUCTURE OF MAMMALIAN SMALL NUCLEAR RIBONUCLEOPROTEINS - IDENTIFICATION OF MULTIPLE PROTEIN-COMPONENTS REACTIVE WITH ANTI-(U1)RIBONUCLEOPROTEIN AND ANTI-SM AUTOANTIBODIES, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 5907 (1984). (10.1016/S0021-9258(18)91101-4) / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
PIKIELNY, C.W., SPECIFIC SMALL NUCLEAR RNAS ARE ASSOCIATED WITH YEAST SPLICEOSOMES, CELL 45: 869 (1986). (10.1016/0092-8674(86)90561-1) / CELL (1986)
REDDY, R, NUCLEOTIDE-SEQUENCE OF NUCLEOLAR U3B RNA, JOURNAL OF BIOLOGICAL CHEMISTRY 254: 11097 (1979). (10.1016/S0021-9258(19)86635-8) / JOURNAL OF BIOLOGICAL CHEMISTRY (1979)
REDDY, R, ISOLATION AND PARTIAL CHARACTERIZATION OF DINOFLAGELLATE U1-U6 SMALL RNAS HOMOLOGOUS TO RAT U-SMALL NUCLEAR RNAS, JOURNAL OF BIOLOGICAL CHEMISTRY 258: 13965 (1983). (10.1016/S0021-9258(17)44010-5) / JOURNAL OF BIOLOGICAL CHEMISTRY (1983)
RIEDEL, N, SMALL NUCLEAR RNAS FROM SACCHAROMYCES-CEREVISIAE - UNEXPECTED DIVERSITY IN ABUNDANCE, SIZE, AND MOLECULAR COMPLEXITY, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 8097 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
RIEDEL N unpublished data.
ROTHSTEIN, R.J., ONE-STEP GENE DISRUPTION IN YEAST, METHODS IN ENZYMOLOGY 101: 202 (1983). (10.1016/0076-6879(83)01015-0) / METHODS IN ENZYMOLOGY (1983)
SILICIANO P unpublished data.
STRUB, K, THE CDNA SEQUENCES OF THE SEA-URCHIN U7 SMALL NUCLEAR-RNA SUGGEST SPECIFIC CONTACTS BETWEEN HISTONE MESSENGER-RNA PRECURSOR AND U7 RNA DURING RNA PROCESSING, EMBO JOURNAL 3: 2801 (1984). (10.1002/j.1460-2075.1984.tb02212.x) / EMBO JOURNAL (1984)
TOLLERVEY, D, A U4-LIKE SMALL NUCLEAR-RNA IS DISPENSABLE IN YEAST, CELL 35: 753 (1983). (10.1016/0092-8674(83)90108-3) / CELL (1983)
TOLLERVEY, D, DELETION OF A YEAST SMALL NUCLEAR-RNA GENE IMPAIRS GROWTH, EMBO JOURNAL 4: 3873 (1985). (10.1002/j.1460-2075.1985.tb04160.x) / EMBO JOURNAL (1985)
WIEBEN, E.D., RIBONUCLEOPROTEIN ORGANIZATION OF EUKARYOTIC RNA .20. SMALL NUCLEAR RIBONUCLEOPROTEINS OF DROSOPHILA - IDENTIFICATION OF U1 RNA-ASSOCIATED PROTEINS AND THEIR BEHAVIOR DURING HEAT-SHOCK, MOLECULAR AND CELLULAR BIOLOGY 2: 914 (1982). / MOLECULAR AND CELLULAR BIOLOGY (1982)
WISE, J.A., YEAST CONTAINS SMALL NUCLEAR RNAS ENCODED BY SINGLE COPY GENES, CELL 35: 743 (1983). (10.1016/0092-8674(83)90107-1) / CELL (1983)
WOLIN, S.L., THE RO SMALL CYTOPLASMIC RIBONUCLEOPROTEINS - IDENTIFICATION OF THE ANTIGENIC PROTEIN AND ITS BINDING-SITE ON THE RO RNAS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 81: 1996 (1984). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1984)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:51 a.m.)
Indexed	1 year, 7 months ago (Jan. 13, 2024, 11:01 p.m.)
Issued	38 years, 7 months ago (Jan. 16, 1987)
Published	38 years, 7 months ago (Jan. 16, 1987)
Published Print	38 years, 7 months ago (Jan. 16, 1987)

Funders 0

None

BibTeX

@article{Riedel_1987, title={A Subset of Yeast snRNA’s Contains Functional Binding Sites for the Highly Conserved Sm Antigen}, volume={235}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2948278}, DOI={10.1126/science.2948278}, number={4786}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Riedel, Nora and Wolin, Sandra and Guthrie, Christine}, year={1987}, month=jan, pages={328–331} }

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