DOI: 10.1126/science.2928784. Prevention of Rapid Intracellular Degradation of ODC by a Carboxyl-Terminal Truncation

Prevention of Rapid Intracellular Degradation of ODC by a Carboxyl-Terminal Truncation

10.1126/science.2928784

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Ornithine decarboxylase (ODC) was converted from a protein with a short intracellular half-life in mammalian cells to a stable protein by truncating 37 residues at its carboxyl terminus. Cells expressing wild-type protein lost ODC activity with a half-life of approximately 1 hour. Cells expressing the truncated protein, however, retained full activity for at least 4 hours. Pulse-chase experiments in which immunoprecipitation and gel electrophoresis were used confirmed the stabilizing effect of the truncation. Thus, a carboxyl-terminal domain is responsible for the rapid intracellular degradation of murine ODC.

Bibliography

Ghoda, L., van Daalen Wetters, T., Macrae, M., Ascherman, D., & Coffino, P. (1989). Prevention of Rapid Intracellular Degradation of ODC by a Carboxyl-Terminal Truncation. Science, 243(4897), 1493â1495.

Authors 5

L. Ghoda (first)
T. van Daalen Wetters (additional)
M. Macrae (additional)
D. Ascherman (additional)
P. Coffino (additional)

References 33 Referenced 196

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:14 a.m.)
Indexed	1 month, 2 weeks ago (July 14, 2025, 11:34 p.m.)
Issued	36 years, 5 months ago (March 17, 1989)
Published	36 years, 5 months ago (March 17, 1989)
Published Print	36 years, 5 months ago (March 17, 1989)

Funders 0

None

BibTeX

@article{Ghoda_1989, title={Prevention of Rapid Intracellular Degradation of ODC by a Carboxyl-Terminal Truncation}, volume={243}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2928784}, DOI={10.1126/science.2928784}, number={4897}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Ghoda, L. and van Daalen Wetters, T. and Macrae, M. and Ascherman, D. and Coffino, P.}, year={1989}, month=mar, pages={1493–1495} }

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