Sterility of Drosophila with Mutations in the Bloom Syndrome Gene--Complementation by Ku70
10.1126/science.291.5513.2600
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The Drosophila Dmblm locus is a homolog of the human Bloom syndrome gene, which encodes a helicase of the RECQ family. We show that Dmblm is identical to mus309 , a locus originally identified in a mutagen-sensitivity screen. One mus309 allele, which carries a stop codon between two of the helicase motifs, causes partial male sterility and complete female sterility. Mutant males produce an excess of XY sperm and nullo sperm, consistent with a high frequency of nondisjunction and/or chromosome loss. These phenotypes of mus309 suggest that Dmblm functions in DNA double-strand break repair. The mutant Dmblm phenotypes were partially rescued by an extra copy of the DNA repair gene Ku70 , indicating that the two genes functionally interact in vivo.

Bibliography

Kusano, K., Johnson-Schlitz, D. M., & Engels, W. R. (2001). Sterility of Drosophila with Mutations in the Bloom Syndrome Gene--Complementation by Ku70. Science, 291(5513), 2600–2602.

Authors 3
  1. Kohji Kusano (first)
  2. Dena M. Johnson-Schlitz (additional)
  3. William R. Engels (additional)
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  29. Supplementary material is available on Science Online at www.sciencemag.org/cgi/content/full/291/5513/2600/DC1
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Dates
Type When
Created 23 years ago (July 27, 2002, 5:52 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 4:34 a.m.)
Indexed 2 weeks, 6 days ago (Aug. 6, 2025, 8:45 a.m.)
Issued 24 years, 4 months ago (March 30, 2001)
Published 24 years, 4 months ago (March 30, 2001)
Published Print 24 years, 4 months ago (March 30, 2001)
Funders 0

None

@article{Kusano_2001, title={Sterility of Drosophila with Mutations in the Bloom Syndrome Gene--Complementation by Ku70}, volume={291}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.291.5513.2600}, DOI={10.1126/science.291.5513.2600}, number={5513}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kusano, Kohji and Johnson-Schlitz, Dena M. and Engels, William R.}, year={2001}, month=mar, pages={2600–2602} }