DOI: 10.1126/science.291.5503.495. Loss of Insulator Activity by Paired Su(Hw) Chromatin Insulators

Loss of Insulator Activity by Paired Su(Hw) Chromatin Insulators

10.1126/science.291.5503.495

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Chromatin insulators are regulatory elements that block the action of transcriptional enhancers when interposed between enhancer and promoter. The Drosophila Suppressor of Hairy wing [Su(Hw)] protein binds the Su(Hw) insulator and prevents enhancer-promoter interaction by a mechanism that is not understood. We show that when two copies of the Su(Hw) insulator element, instead of a single one, are inserted between enhancer and promoter, insulator activity is neutralized and the enhancer-promoter interaction may instead be facilitated. This paradoxical phenomenon could be explained by interactions between protein complexes bound at the insulators.

Bibliography

Muravyova, E., Golovnin, A., Gracheva, E., Parshikov, A., Belenkaya, T., Pirrotta, V., & Georgiev, P. (2001). Loss of Insulator Activity by Paired Su(Hw) Chromatin Insulators. Science, 291(5503), 495â498.

Authors 7

Ekaterina Muravyova (first)
Anton Golovnin (additional)
Elena Gracheva (additional)
Aleksander Parshikov (additional)
Tatiana Belenkaya (additional)
Vincenzo Pirrotta (additional)
Pavel Georgiev (additional)

References 22 Referenced 169

H. Cai M. Levine. Nature 376 533 (1995). (10.1038/376533a0)
Geyer P. K., Corces V. G., Genes Dev. 6, 1865 (1992). (10.1101/gad.6.10.1865) / Genes Dev. by Geyer P. K. (1992)
Holdridge C., Dorsett D., Mol. Cell. Biol. 11, 1894 (1991). / Mol. Cell. Biol. by Holdridge C. (1991)
10.1242/dev.113.3.735
Roseman R. R., Pirrotta V., Geyer P. K., EMBO J. 12, 435 (1993). (10.1002/j.1460-2075.1993.tb05675.x) / EMBO J. by Roseman R. R. (1993)
Scott K. S., Geyer P. K., EMBO J. 14, 6258 (1995). (10.1002/j.1460-2075.1995.tb00316.x) / EMBO J. by Scott K. S. (1995)
Gause M., et al., Genetics 149, 1393 (1998). (10.1093/genetics/149.3.1393) / Genetics by Gause M. (1998)
Geyer P. K., Corces V. G., Genes Dev. 1, 996 (1987). (10.1101/gad.1.9.996) / Genes Dev. by Geyer P. K. (1987)
Tabulations of the different lines and genetic crosses with this and other constructs in this report are available at www.sciencemag.org/cgi/content/full/291/5503/495/DC1.
Flp recombinase catalyzes reciprocal recombination between two FRT sequences [
Golic K. G., Lindquist S., Cell 59, 499 (1989)]. (10.1016/0092-8674(89)90033-0) / Cell by Golic K. G. (1989)
Cai H., et al., Science 291, 493 (2000). (10.1126/science.291.5503.493) / Science by Cai H. (2000)
Gerasimova T. I., Gdula D. A., Gerasimov D. V., Simonova O., Corces V. G., Cell 82, 587 (1995). (10.1016/0092-8674(95)90031-4) / Cell by Gerasimova T. I. (1995)
Gerasimova T. I., Corces V. G., Cell 92, 511 (1998). (10.1016/S0092-8674(00)80944-7) / Cell by Gerasimova T. I. (1998)
Mihaly J., et al., Cell. Mol. Life Sci. 54, 60 (1998). (10.1007/s000180050125) / Cell. Mol. Life Sci. by Mihaly J. (1998)
Hagstrom K., Müller M., Schedl P., Genes Dev. 10, 3202 (1996). (10.1101/gad.10.24.3202) / Genes Dev. by Hagstrom K. (1996)
Zhou J., Barolo S., Szymanski P., Levine M., Genes Dev. 10, 3195 (1996). (10.1101/gad.10.24.3195) / Genes Dev. by Zhou J. (1996)
Zhou J., Levine M., Cell 99, 567 (1999). (10.1016/S0092-8674(00)81546-9) / Cell by Zhou J. (1999)
Qian S., Varjavand B., Pirrotta V., Genetics 131, 79 (1992). (10.1093/genetics/131.1.79) / Genetics by Qian S. (1992)
Georgiev P., Kozycina M., Genetics 142, 425 (1996). (10.1093/genetics/142.2.425) / Genetics by Georgiev P. (1996)
The transposon constructs were based on the CaSpeR series and derivatives. The entire yellow gene was contained in an 8-kb fragment with the partially overlapping body and wing enhancers located respectively at positions −1266 to −1963 and −1808 to −2873 from the transcription start site. The white Eye enhancer fragment contained eye and testis enhancers (18). The Su(Hw) insulator was a 430-bp fragment containing 12 Su(Hw) binding sites amplified by polymerase chain reaction from the gypsy retrotransposon. Details of the constructions are available upon request. The constructs were injected in y − ac − w 1118 embryos and the transgenic flies were identified by their eye color. The transformed lines were tested by Southern blot hybridization for transposon integrity copy number and presence of the enhancers and Su(Hw) insulators. Only lines with single-copy inserts were used. Lines in a su(Hw) − mutant background were obtained by consecutively crossing transgenic males with C(1)RM yf ; D / T(2;3)Xa C(1)RM yf ; su(Hw) v /T(2;3)Xa C(1)RM yf ; su(Hw) 2 sbd/T(2;3)Xa females as described (19).
Supported by grants from the Human Frontiers Science Program and from INTAS to P.G. and V.P. P.G. was an International Research Scholar of the Howard Hughes Medical Institute and received an award from the Volkswagen Stiftung Foundation and A.G. was supported by a stipend from the Center for Medical Studies University of Oslo.

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:53 a.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:31 a.m.)
Indexed	1 month ago (July 30, 2025, 10:19 a.m.)
Issued	24 years, 7 months ago (Jan. 19, 2001)
Published	24 years, 7 months ago (Jan. 19, 2001)
Published Print	24 years, 7 months ago (Jan. 19, 2001)

Funders 0

None

BibTeX

@article{Muravyova_2001, title={Loss of Insulator Activity by Paired Su(Hw) Chromatin Insulators}, volume={291}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.291.5503.495}, DOI={10.1126/science.291.5503.495}, number={5503}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Muravyova, Ekaterina and Golovnin, Anton and Gracheva, Elena and Parshikov, Aleksander and Belenkaya, Tatiana and Pirrotta, Vincenzo and Georgiev, Pavel}, year={2001}, month=jan, pages={495–498} }

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