DOI: 10.1126/science.290.5496.1555. Powering an Inorganic Nanodevice with a Biomolecular Motor

Powering an Inorganic Nanodevice with a Biomolecular Motor

10.1126/science.290.5496.1555

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Biomolecular motors such as F 1 –adenosine triphosphate synthase (F 1 -ATPase) and myosin are similar in size, and they generate forces compatible with currently producible nanoengineered structures. We have engineered individual biomolecular motors and nanoscale inorganic systems, and we describe their integration in a hybrid nanomechanical device powered by a biomolecular motor. The device consisted of three components: an engineered substrate, an F 1 -ATPase biomolecular motor, and fabricated nanopropellers. Rotation of the nanopropeller was initiated with 2 mM adenosine triphosphate and inhibited by sodium azide.

Bibliography

Soong, R. K., Bachand, G. D., Neves, H. P., Olkhovets, A. G., Craighead, H. G., & Montemagno, C. D. (2000). Powering an Inorganic Nanodevice with a Biomolecular Motor. Science, 290(5496), 1555â1558.

Authors 6

Ricky K. Soong (first)
George D. Bachand (additional)
Hercules P. Neves (additional)
Anatoli G. Olkhovets (additional)
Harold G. Craighead (additional)
Carlo D. Montemagno (additional)

References 25 Referenced 553

Marcus R. B., Ravi T. S., Gmitter T., Appl. Phys. Lett. 56, 236 (1990). (10.1063/1.102841) / Appl. Phys. Lett. by Marcus R. B. (1990)
Sone J., et al., Nanotechnology 10, 135 (1999). (10.1088/0957-4484/10/2/306) / Nanotechnology by Sone J. (1999)
Carr D. W., Sekaric L., Craighead H. G., J. Vac. Sci. Technol. B 16, 3821 (1998). (10.1116/1.590416) / J. Vac. Sci. Technol. B by Carr D. W. (1998)
Carr D. W., Evoy S., Sekaric L., Parpia J. M., Craighead H. G., Appl. Phys. Lett. 75, 920 (1999). (10.1063/1.124554) / Appl. Phys. Lett. by Carr D. W. (1999)
Montemagno C. D., Bachand G. D., Nanotechnology 10, 225 (1999). (10.1088/0957-4484/10/3/301) / Nanotechnology by Montemagno C. D. (1999)
Bachand G. D., Montemagno C. D., Biomed. Microdevices 2, 179 (2000). (10.1023/A:1009924327649) / Biomed. Microdevices by Bachand G. D. (2000)
10.1016/S0092-8674(00)81138-1
10.1038/41111
10.1126/science.282.5390.902
10.1038/16403
10.1016/S0092-8674(00)81456-7
10.1038/386299a0
R. K. Soong S. J. Stelick G. D. Bachand C. D. Montemagno Technical Proceedings of the Second International Conference on Modeling and Simulation of Microsystems San Juan Puerto Rico 19–21 April 1999 (Computational Publications Boston 1999) pp. 95–98.
Site-directed mutagenesis was used to (i) change the α initiation codon from ATG to CTG (ii) change the αCys 193 to Ser (iii) change the γSer 107 to Cys (iv) change the γ initiation codon from GTG to ATG and (v) change the γ termination codon from TAG to TAA in the cloned F 1 -ATPase construct (pGEM-DEMH). The construct also contained a 10-histidine tag that was inserted immediately downstream of the β initiation codon. The recombinant protein was expressed in E. coli JM103 Δ( uncB-uncD ) and purified using affinity (i.e. Ni–nitrilotriacetic acid) and size exclusion chromatography.
Matsui T., Yoshida M., Biochim. Biophys. Acta 1231, 139 (1995). (10.1016/0005-2728(95)00070-Y) / Biochim. Biophys. Acta by Matsui T. (1995)
The exposure pattern written on the cover slip consisted of dots with a 5-μm horizontal and vertical pitch and border marks to identify the post region. The dots averaged 50 to 120 nm in diameter depending on the e-beam exposure dose which varied from 10 to 30 fC/μm 2 . The exposed area measured 300 μm by 300 μm exclusive of the border marks. The border marks (lines 50 μm wide) were spaced 0.5 μm from the edge of the post region.
The patterning was performed on silicon wafers that were spin-coated with 4% PMMA in methoxybenzene at 3000 rpm resulting in a thickness of ∼150 nm. The width of the nanopropellers was 150 nm; their length varied from 750 nm to 1.4 μm depending on the exposure dose which varied from 3.75 to 4.5 nC/cm. The optimum dose was determined to be 4.3 nC/cm.
The synthetic His-rich peptide was specifically biotinylated to the NH 2 -terminal Cys residue through disulfide linkage in the presence of N N -dimethylformamide. A 100-μl aliquot of the biotinylated peptide was incubated with 400 μl of the nanopropellers for 15 min then dialyzed against 1× PBS to remove any excess peptide and biotin maleimide.
Jeffrey D. J., Onishi Y., J. Mech. Appl. Math. 34, 129 (1981). (10.1093/qjmam/34.2.129) / J. Mech. Appl. Math. by Jeffrey D. J. (1981)
10.1016/S0006-3495(94)80537-5
L. Stryer Biochemistry (Freeman New York ed. 4 1999) pp. 445–447.
10.1073/pnas.96.14.7780
Noji H., et al., Biochem. Biophys. Res. Commun. 260, 597 (1999). (10.1006/bbrc.1999.0885) / Biochem. Biophys. Res. Commun. by Noji H. (1999)
Hisabori T., Kondoh A., Yoshida M., FEBS Lett. 463, 35 (1999). (10.1016/S0014-5793(99)01602-6) / FEBS Lett. by Hisabori T. (1999)
We thank F. Peters for figure preparation and assistance in digitization of the data. Supported by grants from the Keck Fellowship Program Office of Naval Research and Defense Advanced Research Projects Agency (N00014-99-1-0436CS) NSF (ECS-9007033 and ECS-7876771) U.S. Department of Energy (DE-FG07-96ER14703) and NASA (NAG5-8775).

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:52 a.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 5:39 a.m.)
Indexed	1 month, 2 weeks ago (July 16, 2025, 9:47 a.m.)
Issued	24 years, 9 months ago (Nov. 24, 2000)
Published	24 years, 9 months ago (Nov. 24, 2000)
Published Print	24 years, 9 months ago (Nov. 24, 2000)

Funders 0

None

BibTeX

@article{Soong_2000, title={Powering an Inorganic Nanodevice with a Biomolecular Motor}, volume={290}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.290.5496.1555}, DOI={10.1126/science.290.5496.1555}, number={5496}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Soong, Ricky K. and Bachand, George D. and Neves, Hercules P. and Olkhovets, Anatoli G. and Craighead, Harold G. and Montemagno, Carlo D.}, year={2000}, month=nov, pages={1555–1558} }

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