DOI: 10.1126/science.290.5495.1354. Regulation of Antigen-Specific CD8 <sup>+</sup> T Cell Homeostasis by Perforin and Interferon-γ

Regulation of Antigen-Specific CD8 + T Cell Homeostasis by Perforin and Interferon-γ

10.1126/science.290.5495.1354

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

T cell memory depends on factors that regulate expansion and death of these cells after antigenic stimulation. Mice deficient in perforin and interferon-γ (IFN-γ) exhibited increased expansion, altered immunodominance, and decreased death of antigen-specific CD8 + T cells after infection with an attenuated strain of Listeria monocytogenes , which was cleared from these mice. Expansion of CD8 + T cells was controlled by perforin, whereas IFN-γ regulated immunodominance and the death phase. Thus, perforin and IFN-γ regulate distinct elements of CD8 + T cell homeostasis independently of their role as antimicrobial effector molecules.

Bibliography

Badovinac, V. P., Tvinnereim, A. R., & Harty, J. T. (2000). Regulation of Antigen-Specific CD8 + T Cell Homeostasis by Perforin and Interferon-Î³. Science, 290(5495), 1354â1357.

Authors 3

Vladimir P. Badovinac (first)
Amy R. Tvinnereim (additional)
John T. Harty (additional)

References 42 Referenced 366

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Attenuated LM strain DP-L1942 (ActA-deficient) (3) was prepared for injection and colony forming units (CFU) per spleen or per grams of liver were determined as previously described (4).
The magnitude of the epitope-specific CD8 + T cell response was determined by ICS for TNF IFN-γ or MHC class I tetramer staining as previously described (5 11 15). For ICS the percent of TNF + or IFN-γ + CD8 + T cells in the unstimulated sample for each mouse was subtracted from the peptide stimulated value to determine the frequency of Ag-specific CD8 + T cells. Total number of epitope-specific CD8 + cells per spleen was calculated from this frequency the percent of CD8 + T cells in each sample and total number of cells per spleen.
10.1038/353852a0
E. G. Pamer J. Immunol. 152 686 (1994). (10.4049/jimmunol.152.2.686)
BALB/c bone marrow–derived dendritic cells prepared by incubation for 6 days in granulocyte-macrophage colony-stimulating factor (GM-CSF) + interleukin-4 (IL-4) (36) were matured by incubation with lipopolysaccharide (50 ng/ml) for 48 hours. LLO 91-99 and OVA 323-336 peptides (1 μM final concentration) were added for the last 24 hours of culture.
We thank L. Gorton and G. Corbin for excellent technical assistance J. Mahoney and D. Bonthius for assistance with LCMV titrations the National Institute of Allergy and Infectious Diseases MHC Tetramer Core Facility for MHC Class I tetramers and S. Perlman and T. Waldschmidt for critical review of the manuscript. Supported by NIH grants AI36864 AI42767 T32AI07511 and T32AI07260.

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:47 a.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 3:57 a.m.)
Indexed	2 months, 3 weeks ago (June 2, 2025, 3:28 p.m.)
Issued	24 years, 9 months ago (Nov. 17, 2000)
Published	24 years, 9 months ago (Nov. 17, 2000)
Published Print	24 years, 9 months ago (Nov. 17, 2000)

Funders 0

None

BibTeX

@article{Badovinac_2000, title={Regulation of Antigen-Specific CD8 + T Cell Homeostasis by Perforin and Interferon-γ}, volume={290}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.290.5495.1354}, DOI={10.1126/science.290.5495.1354}, number={5495}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Badovinac, Vladimir P. and Tvinnereim, Amy R. and Harty, John T.}, year={2000}, month=nov, pages={1354–1357} }

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