DOI: 10.1126/science.2898810. Purification and Characterization of Mouse Hematopoietic Stem Cells

Purification and Characterization of Mouse Hematopoietic Stem Cells

10.1126/science.2898810

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Mouse bone marrow hematopoietic stem cells were isolated with the use of a variety of phenotypic markers. These cells can proliferate and differentiate with approximately unit efficiency into myelomonocytic cells, B cells, or T cells. Thirty of these cells are sufficient to save 50 percent of lethally irradiated mice, and to reconstitute all blood cell types in the survivors.

Bibliography

Spangrude, G. J., Heimfeld, S., & Weissman, I. L. (1988). Purification and Characterization of Mouse Hematopoietic Stem Cells. Science, 241(4861), 58â62.

Authors 3

Gerald J. Spangrude (first)
Shelly Heimfeld (additional)
Irving L. Weissman (additional)

References 30 Referenced 2,077

ABRAMSON, S, IDENTIFICATION IN ADULT BONE-MARROW OF PLURIPOTENT AND RESTRICTED STEM-CELLS OF MYELOID AND LYMPHOID SYSTEMS, JOURNAL OF EXPERIMENTAL MEDICINE 145: 1567 (1977). (10.1084/jem.145.6.1567) / JOURNAL OF EXPERIMENTAL MEDICINE (1977)
AIHARA, Y, AN ATTEMPT TO PRODUCE PRE-T CELL HYBRIDOMAS AND TO IDENTIFY THEIR ANTIGENS, EUROPEAN JOURNAL OF IMMUNOLOGY 16: 1391 (1986). (10.1002/eji.1830161113) / EUROPEAN JOURNAL OF IMMUNOLOGY (1986)
BERMAN, J.W., THY-1 ANTIGEN EXPRESSION BY MURINE HEMATOPOIETIC PRECURSOR CELLS, EXPERIMENTAL HEMATOLOGY 13: 1152 (1985). / EXPERIMENTAL HEMATOLOGY (1985)
COFFMAN, R.L., B220 - A B-CELL-SPECIFIC MEMBER OF THE T200 GLYCOPROTEIN FAMILY, NATURE 289: 681 (1981). (10.1038/289681a0) / NATURE (1981)
10.1038/177452a0
GOLDSCHNEIDER, I, DEMONSTRATION OF THY-1 ANTIGEN ON PLURIPOTENT HEMATOPOIETIC STEM-CELLS IN RAT, JOURNAL OF EXPERIMENTAL MEDICINE 148: 1351 (1978). (10.1084/jem.148.5.1351) / JOURNAL OF EXPERIMENTAL MEDICINE (1978)
HEIMFELD S unpublished data.
HENDRY, J.H., F NUMBER OF PRIMARY TRANSPLANTED SPLENIC COLONY-FORMING CELLS, CELL AND TISSUE KINETICS 4: 217 (1971). / CELL AND TISSUE KINETICS (1971)
HODGSON, G.S., PROPERTIES OF HEMATOPOIETIC STEM-CELLS SURVIVING 5-FLUOROURACIL TREATMENT - EVIDENCE FOR A PRE-CFU-S CELL, NATURE 281: 381 (1979). (10.1038/281381a0) / NATURE (1979)
KATSURA, Y, LIMITING DILUTION ANALYSIS OF THE STEM-CELLS FOR T-CELL LINEAGE, JOURNAL OF IMMUNOLOGY 137: 2434 (1986). (10.4049/jimmunol.137.8.2434) / JOURNAL OF IMMUNOLOGY (1986)
LEMISCHKA, I.R., DEVELOPMENTAL POTENTIAL AND DYNAMIC BEHAVIOR OF HEMATOPOIETIC STEM-CELLS, CELL 45: 917 (1986). (10.1016/0092-8674(86)90566-0) / CELL (1986)
LORD, B.I., RELATIONSHIP BETWEEN SPLEEN COLONY PRODUCTION AND SPLEEN CELLULARITY, CELL AND TISSUE KINETICS 4: 211 (1971). / CELL AND TISSUE KINETICS (1971)
MCCUNE M unpublished data.
Micklem H. S. Tissue Grafiing and Radiation (1966).
MOLINEUX, G, DEVELOPMENT OF SPLEEN CFU-S COLONIES FROM DAY-8 TO DAY-11 - RELATIONSHIP TO SELF-RENEWAL CAPACITY, EXPERIMENTAL HEMATOLOGY 14: 710 (1986). / EXPERIMENTAL HEMATOLOGY (1986)
MOORE, MAS, STEM-CELL MIGRATION IN DEVELOPING MYELOID AND LYMPHOID SYSTEMS, LANCET 2: 658 (1967). / LANCET (1967)
MULLERSIEBURG, C.E., ISOLATION OF 2 EARLY LYMPHOCYTE-B PROGENITORS FROM MOUSE MARROW - A COMMITTED PRE-PRE-B-CELL AND A CLONOGENIC THY-1LO HEMATOPOIETIC STEM-CELL, CELL 44: 653 (1986). (10.1016/0092-8674(86)90274-6) / CELL (1986)
Müller-Sieburg, C. E., Journal of Experimental Medicine 167: 1825 (1988). (10.1084/jem.167.6.1825) / Journal of Experimental Medicine (1988)
Muller-Sieburg C. E. Thy-1: Immunology Neurology and Therapeutic Applications (1989).
PARKS, D.R., FLUORESCENCE-ACTIVATED CELL SORTING - THEORY, EXPERIMENTAL OPTIMIZATION, AND APPLICATIONS IN LYMPHOID-CELL BIOLOGY, METHODS IN ENZYMOLOGY 108: 197 (1984). (10.1016/S0076-6879(84)08086-1) / METHODS IN ENZYMOLOGY (1984)
SCHEID, M.P., FURTHER DESCRIPTION OF THE LY-5 SYSTEM, IMMUNOGENETICS 9: 423 (1979). (10.1007/BF01570435) / IMMUNOGENETICS (1979)
SIMINOVITCH, L, JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY 62: 327 (1963). (10.1002/jcp.1030620313) / JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY (1963)
Spangrude, G. J., Journal of Experimental Medicine 167: 1671 (1988). (10.1084/jem.167.5.1671) / Journal of Experimental Medicine (1988)
SPANGRUDE G.J. unpublished data.
TILL, J.E., DIRECT MEASUREMENT OF RADIATION SENSITIVITY OF NORMAL MOUSE BONE MARROW CELLS, RADIATION RESEARCH 14: 213 (1961). (10.2307/3570892) / RADIATION RESEARCH (1961)
TILL, J.E., SERIES HAEMATOLOGICA 5: 15 (1972). / SERIES HAEMATOLOGICA (1972)
VISSER, J. W. M., JOURNAL OF EXPERIMENTAL MEDICINE 159: 1576 (1984). (10.1084/jem.159.6.1576) / JOURNAL OF EXPERIMENTAL MEDICINE (1984)
WHITLOCK, C.A., BONE-MARROW STROMAL CELL-LINES WITH LYMPHOPOIETIC ACTIVITY EXPRESS HIGH-LEVELS OF A PRE-B NEOPLASIA-ASSOCIATED MOLECULE, CELL 48: 1009 (1987). (10.1016/0092-8674(87)90709-4) / CELL (1987)
Whitlock, C. A., Proceedings of the National Academy of Sciences of the United States of America 79: 3608 (1982). / Proceedings of the National Academy of Sciences of the United States of America (1982)
WU, A.M., CYTOLOGICAL STUDY OF CAPACITY FOR DIFFERENTIATION OF NORMAL HEMOPOIETIC COLONY-FORMING CELLS, JOURNAL OF CELLULAR PHYSIOLOGY 69: 177 (1967). (10.1002/jcp.1040690208) / JOURNAL OF CELLULAR PHYSIOLOGY (1967)

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:26 a.m.)
Indexed	3 days, 21 hours ago (Aug. 22, 2025, 12:47 a.m.)
Issued	37 years, 1 month ago (July 1, 1988)
Published	37 years, 1 month ago (July 1, 1988)
Published Print	37 years, 1 month ago (July 1, 1988)

Funders 0

None

BibTeX

@article{Spangrude_1988, title={Purification and Characterization of Mouse Hematopoietic Stem Cells}, volume={241}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2898810}, DOI={10.1126/science.2898810}, number={4861}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Spangrude, Gerald J. and Heimfeld, Shelly and Weissman, Irving L.}, year={1988}, month=jul, pages={58–62} }

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