DOI: 10.1126/science.2897716. Chloroplast Transformation in <i>Chlamydomonas</i> with High Velocity Microprojectiles

Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles

10.1126/science.2897716

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Bombardment of three mutants of the chloroplast atpB gene of Chlamydomonas reinhardtii with high-velocity tungsten microprojectiles that were coated with cloned chloroplast DNA carrying the wild-type gene permanently restored the photosynthetic capacity of the algae. In most transformants of one of the mutants, a fragment with a 2.5-kilobase deletion was restored to normal size by a homologous replacement event; in about 25 percent of the transformants the restored restriction fragment was 50 to 100 base pairs smaller or larger than that of wild type. About one-fourth of the transformants of this mutant contained unintegrated donor plasmid when first examined. This plasmid persisted in four different transformants after 65 cell generations of continuous liquid culture but was lost from all transformants maintained on plates of selective medium. The restored wild-type atpB gene remains in all transformants as an integral part of the chloroplast genome and is expressed and inherited normally.

Bibliography

Boynton, J. E., Gillham, N. W., Harris, E. H., Hosler, J. P., Johnson, A. M., Jones, A. R., Randolph-Anderson, B. L., Robertson, D., Klein, T. M., Shark, K. B., & Sanford, J. C. (1988). Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles. Science, 240(4858), 1534â1538.

Authors 11

John E. Boynton (first)
Nicholas W. Gillham (additional)
Elizabeth H. Harris (additional)
Jonathan P. Hosler (additional)
Anita M. Johnson (additional)
Allan R. Jones (additional)
Barbara L. Randolph-Anderson (additional)
Dominique Robertson (additional)
Ted M. Klein (additional)
Katherine B. Shark (additional)
John C. Sanford (additional)

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:52 a.m.)
Indexed	2 weeks, 6 days ago (Aug. 7, 2025, 4:21 p.m.)
Issued	37 years, 2 months ago (June 10, 1988)
Published	37 years, 2 months ago (June 10, 1988)
Published Print	37 years, 2 months ago (June 10, 1988)

Funders 0

None

BibTeX

@article{Boynton_1988, title={Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles}, volume={240}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2897716}, DOI={10.1126/science.2897716}, number={4858}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Boynton, John E. and Gillham, Nicholas W. and Harris, Elizabeth H. and Hosler, Jonathan P. and Johnson, Anita M. and Jones, Allan R. and Randolph-Anderson, Barbara L. and Robertson, Dominique and Klein, Ted M. and Shark, Katherine B. and Sanford, John C.}, year={1988}, month=jun, pages={1534–1538} }

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