Three-Dimensional Structure of the Tn 5 Synaptic Complex Transposition Intermediate
10.1126/science.289.5476.77
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Genomic evolution has been profoundly influenced by DNA transposition, a process whereby defined DNA segments move freely about the genome. Transposition is mediated by transposases, and similar events are catalyzed by retroviral integrases such as human immunodeficiency virus–1 (HIV-1) integrase. Understanding how these proteins interact with DNA is central to understanding the molecular basis of transposition. We report the three-dimensional structure of prokaryotic Tn 5 transposase complexed with Tn 5 transposon end DNA determined to 2.3 angstrom resolution. The molecular assembly is dimeric, where each double-stranded DNA molecule is bound by both protein subunits, orienting the transposon ends into the active sites. This structure provides a molecular framework for understanding many aspects of transposition, including the binding of transposon end DNA by one subunit and cleavage by a second, cleavage of two strands of DNA by a single active site via a hairpin intermediate, and strand transfer into target DNA.

Bibliography

Davies, D. R., Goryshin, I. Y., Reznikoff, W. S., & Rayment, I. (2000). Three-Dimensional Structure of the Tn 5 Synaptic Complex Transposition Intermediate. Science, 289(5476), 77–85.

Authors 4
  1. Douglas R. Davies (first)
  2. Igor Y. Goryshin (additional)
  3. William S. Reznikoff (additional)
  4. Ivan Rayment (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:35 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 5:39 a.m.)
Indexed 6 days, 18 hours ago (Aug. 30, 2025, 1:02 p.m.)
Issued 25 years, 1 month ago (July 7, 2000)
Published 25 years, 1 month ago (July 7, 2000)
Published Print 25 years, 1 month ago (July 7, 2000)
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@article{Davies_2000, title={Three-Dimensional Structure of the Tn 5 Synaptic Complex Transposition Intermediate}, volume={289}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.289.5476.77}, DOI={10.1126/science.289.5476.77}, number={5476}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Davies, Douglas R. and Goryshin, Igor Y. and Reznikoff, William S. and Rayment, Ivan}, year={2000}, month=jul, pages={77–85} }