DOI: 10.1126/science.288.5471.1643. Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression

Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression

10.1126/science.288.5471.1643

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Little is known about the DNA helicases required for the elongation phase of eukaryotic chromosome replication. Minichromosome maintenance (MCM) protein complexes have DNA helicase activity but have only been functionally implicated in initiating DNA replication. Using an improved method for constructing conditional degron mutants, we show that depletion of MCMs after initiation irreversibly blocks the progression of replication forks in Saccharomyces cerevisiae . Like the Escherichia coli dnaB and SV40 T antigen helicases, therefore, the MCM complex is loaded at origins before initiation and is essential for elongation. Restricting MCM loading to the G 1 phase ensures that initiation and elongation occur just once per cell cycle.

Bibliography

Labib, K., Tercero, J. A., & Diffley, J. F. X. (2000). Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression. Science, 288(5471), 1643â1647.

Authors 3

Karim Labib (first)
José Antonio Tercero (additional)
John F. X. Diffley (additional)

References 28 Referenced 486

10.1016/S0167-4781(98)00033-5
10.1146/annurev.biochem.68.1.649
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Web figure 1 and information on methods are available at Science Online at www.sciencemag.org/features/data/1048585/shl.
K. Labib J. A. Tercero J. F. X. Diffley data not shown.
10.1101/gad.12.4.480
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10.1126/science.272.5264.1030
10.1038/27001
We thank L. Johnston and A. Varshavsky for providing plasmids K. Bousset for advice concerning density transfer experiments and T. Lindahl for helpful comments on the manuscript. Supported by the Imperial Cancer Research Fund and a postdoctoral fellowship from the Human Frontier Science Programme (J.A.T.).

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:35 a.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 5:23 a.m.)
Indexed	3 weeks, 2 days ago (Aug. 7, 2025, 4:42 p.m.)
Issued	25 years, 2 months ago (June 2, 2000)
Published	25 years, 2 months ago (June 2, 2000)
Published Print	25 years, 2 months ago (June 2, 2000)

Funders 0

None

BibTeX

@article{Labib_2000, title={Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression}, volume={288}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.288.5471.1643}, DOI={10.1126/science.288.5471.1643}, number={5471}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Labib, Karim and Tercero, José Antonio and Diffley, John F. X.}, year={2000}, month=jun, pages={1643–1647} }

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