DOI: 10.1126/science.288.5470.1436. Granuloma-Specific Expression of Mycobacterium Virulence Proteins from the Glycine-Rich PE-PGRS Family

Granuloma-Specific Expression of Mycobacterium Virulence Proteins from the Glycine-Rich PE-PGRS Family

10.1126/science.288.5470.1436

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Pathogenic mycobacteria, including the agent of tuberculosis, Mycobacterium tuberculosis , must replicate in macrophages for long-term persistence within their niche during chronic infection: organized collections of macrophages and lymphocytes called granulomas. We identified several genes preferentially expressed when Mycobacterium marinum , the cause of fish and amphibian tuberculosis, resides in host granulomas and/or macrophages. Two were homologs of M. tuberculosis PE/PE-PGRS genes, a family encoding numerous repetitive glycine-rich proteins of unknown function. Mutation of two PE-PGRS genes produced M. marinum strains incapable of replication in macrophages and with decreased persistence in granulomas. Our results establish a direct role in virulence for some PE-PGRS proteins.

Bibliography

Ramakrishnan, L., Federspiel, N. A., & Falkow, S. (2000). Granuloma-Specific Expression of Mycobacterium Virulence Proteins from the Glycine-Rich PE-PGRS Family. Science, 288(5470), 1436â1439.

Authors 3

Lalita Ramakrishnan (first)
Nancy A. Federspiel (additional)
Stanley Falkow (additional)

References 31 Referenced 349

10.1016/0966-842X(96)81528-9
10.1128/iai.65.4.1497-1504.1997
Shepard C. C., J. Exp. Med. 106, 722 (1957). / J. Exp. Med. by Shepard C. C. (1957)
___, J. Exp. Med. 105, 39 (1956). / J. Exp. Med. by ___ (1956)
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R. H. Valdivia and L. Ramakrishnan Methods Enzymol. in press.
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10.1128/jcm.30.4.942-946.1992
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GenBank accession number .
10.1128/jb.179.18.5862-5868.1997
L. Ramakrishnan N. A. Federspiel S. Falkow data not shown .
10.1128/iai.62.8.3222-3229.1994
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We thank D. Cunanan for technical assistance; T. Knaak and L. Rott for expertise with FACS sorting; R. Valdivia V. Ramakrishnan M. Troll and S. Karlin for technical help and/or discussions; R. Valdivia B. Chang and A. Kawale respectively for pFPV27 and the hsp 60:: gfp and map 24-2:: gfp fusion constructs; D. Monack and N. Ghori for teaching confocal microscopy; L. Raskin for preparing tissue cryosections; S. Kohler for help with interpretation of tissue histopathology; D. Brooks and P. Small for antisera to M. marinum ; and N. Salama M. Troll K. Otteman and T. McDaniel for critical reading of the manuscript. Supported by NIH award K08 AI 01400 (L.R.) and grant AI 32396 (S.F.).

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:40 a.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 5:50 a.m.)
Indexed	1 day, 22 hours ago (Sept. 3, 2025, 5:51 a.m.)
Issued	25 years, 3 months ago (May 26, 2000)
Published	25 years, 3 months ago (May 26, 2000)
Published Print	25 years, 3 months ago (May 26, 2000)

Funders 0

None

BibTeX

@article{Ramakrishnan_2000, title={Granuloma-Specific Expression of Mycobacterium Virulence Proteins from the Glycine-Rich PE-PGRS Family}, volume={288}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.288.5470.1436}, DOI={10.1126/science.288.5470.1436}, number={5470}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Ramakrishnan, Lalita and Federspiel, Nancy A. and Falkow, Stanley}, year={2000}, month=may, pages={1436–1439} }

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