DOI: 10.1126/science.288.5470.1422. Structure and Function of a Human TAF <sub>II</sub> 250 Double Bromodomain Module

Structure and Function of a Human TAF II 250 Double Bromodomain Module

10.1126/science.288.5470.1422

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

TFIID is a large multiprotein complex that initiates assembly of the transcription machinery. It is unclear how TFIID recognizes promoters in vivo when templates are nucleosome-bound. Here, it is shown that TAF II 250, the largest subunit of TFIID, contains two tandem bromodomain modules that bind selectively to multiply acetylated histone H4 peptides. The 2.1 angstrom crystal structure of the double bromodomain reveals two side-by-side, four-helix bundles with a highly polarized surface charge distribution. Each bundle contains an N ɛ -acetyllysine binding pocket at its center, which results in a structure ideally suited for recognition of diacetylated histone H4 tails. Thus, TFIID may be targeted to specific chromatin-bound promoters and may play a role in chromatin recognition.

Bibliography

Jacobson, R. H., Ladurner, A. G., King, D. S., & Tjian, R. (2000). Structure and Function of a Human TAF II 250 Double Bromodomain Module. Science, 288(5470), 1422â1425.

Authors 4

Raymond H. Jacobson (first)
Andreas G. Ladurner (additional)
David S. King (additional)
Robert Tjian (additional)

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Supported in part by grants from the National Institutes of Health The Wellcome Trust and the Burroughs Wellcome Fund. We thank J. Endrizzi for assistance maintaining our diffraction facilities and offering helpful advice. We thank J. Holton for the use of ELVES T. Alber and J. Berger for their insights and observations and T. Earnest and the staff at the Advanced Light Source for their help. We thank T. Handel and her lab for their support D. Koshland Jr. and S. Marqusee for use of the MCS-ITC instrument and M. Haggart for DNA sequencing and oligonucleotide synthesis. We thank D. Rio M. Botchan and B. W. Matthews for their comments on the manuscript.

Dates

Type	When
Created	23 years ago (July 27, 2002, 5:48 a.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 5:49 a.m.)
Indexed	2 weeks, 1 day ago (Aug. 5, 2025, 8:48 a.m.)
Issued	25 years, 2 months ago (May 26, 2000)
Published	25 years, 2 months ago (May 26, 2000)
Published Print	25 years, 2 months ago (May 26, 2000)

Funders 0

None

BibTeX

@article{Jacobson_2000, title={Structure and Function of a Human TAF II 250 Double Bromodomain Module}, volume={288}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.288.5470.1422}, DOI={10.1126/science.288.5470.1422}, number={5470}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Jacobson, Raymond H. and Ladurner, Andreas G. and King, David S. and Tjian, Robert}, year={2000}, month=may, pages={1422–1425} }

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      "unstructured": "Supported in part by grants from the National Institutes of Health The Wellcome Trust and the Burroughs Wellcome Fund. We thank J. Endrizzi for assistance maintaining our diffraction facilities and offering helpful advice. We thank J. Holton for the use of ELVES T. Alber and J. Berger for their insights and observations and T. Earnest and the staff at the Advanced Light Source for their help. We thank T. Handel and her lab for their support D. Koshland Jr. and S. Marqusee for use of the MCS-ITC instrument and M. Haggart for DNA sequencing and oligonucleotide synthesis. We thank D. Rio M. Botchan and B. W. Matthews for their comments on the manuscript."
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