Promoter-Selective Properties of the TBP-Related Factor TRF1
10.1126/science.288.5467.867
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The TATA-binding protein (TBP)–related factor 1 (TRF1) is expressed in a tissue-restricted fashion during Drosophila embryogenesis and may serve as a promoter-specific recognition factor that can replace TBP in regulating transcription. However, bona fide target promoters that would preferentially respond to TRF1 have remained elusive. Polytene chromosome staining, chromatin immunoprecipitation, direct messenger RNA analysis, and transient cotransfection assays identified the Drosophila gene tudor as containing a TRF1-responsive promoter. Reconstituted in vitro transcription reactions and deoxyribonuclease I footprinting assays confirmed the ability of TRF1 to bind preferentially and direct transcription of the tudor gene from an alternate promoter. Thus, metazoans appear to have evolved gene-selective and tissue-specific components of the core transcription machinery to regulate gene expression.

Bibliography

Holmes, M. C., & Tjian, R. (2000). Promoter-Selective Properties of the TBP-Related Factor TRF1. Science, 288(5467), 867–870.

Authors 2
  1. Michael C. Holmes (first)
  2. Robert Tjian (additional)
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  32. We thank R. Freiman C. Inouye A. Ladurner A. Hochheimer J. Ziegelbauer and D. Rio for critical reading and comments on the manuscript; J. Zwicker for collaborating in setting up a reconstituted in vitro transcription system; S. Takada for stably transformed SL2 cell lines and affinity-purified antibodies to TRF1; R. Jacobson and S. Ryu for reagents; and T. O'Brien for helpful scientific discussions and advice. Supported in part by NIH grant CA25417 (R.T.).
Dates
Type When
Created 23 years ago (July 27, 2002, 5:35 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 5:05 a.m.)
Indexed 2 weeks, 4 days ago (Aug. 5, 2025, 8:52 a.m.)
Issued 25 years, 3 months ago (May 5, 2000)
Published 25 years, 3 months ago (May 5, 2000)
Published Print 25 years, 3 months ago (May 5, 2000)
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@article{Holmes_2000, title={Promoter-Selective Properties of the TBP-Related Factor TRF1}, volume={288}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.288.5467.867}, DOI={10.1126/science.288.5467.867}, number={5467}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Holmes, Michael C. and Tjian, Robert}, year={2000}, month=may, pages={867–870} }