DOI: 10.1126/science.2876520. Isolation of a New Virus, HBLV, in Patients with Lymphoproliferative Disorders

Isolation of a New Virus, HBLV, in Patients with Lymphoproliferative Disorders

10.1126/science.2876520

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

A novel human B-lymphotropic virus (HBLV) was isolated from the peripheral blood leukocytes of six individuals: two HTLV-III seropositive patients from the United States (one with AIDS-related lymphoma and one with dermatopathic lymphadenopathy), three HTLV-III seronegative patients from the United States (one with angioimmunoblastic lymphadenopathy, one with cutaneous T-cell lymphoma, and one with immunoblastic lymphoma), and one HTLV-III seronegative patient with acute lymphocytic leukemia from Jamaica. All six isolates were closely related by antigenic analysis, and sera from all six virus-positive patients reacted immunologically with each virus isolate. In contrast, only four sera from 220 randomly selected healthy donors and none from 12 AIDS patients without associated lymphoma were seropositive. The virus selectively infected freshly isolated human B cells and converted them into large, refractile mono- or binucleated cells with nuclear and cytoplasmic inclusion bodies. HBLV is morphologically similar to viruses of the herpesvirus family but is readily distinguishable from the known human and nonhuman primate herpesviruses by host range, in vitro biological effects, and antigenic features.

Bibliography

Salahuddin, S. Z., Ablashi, D. V., Markham, P. D., Josephs, S. F., Sturzenegger, S., Kaplan, M., Halligan, G., Biberfeld, P., Wong-Staal, F., Kramarsky, B., & Gallo, R. C. (1986). Isolation of a New Virus, HBLV, in Patients with Lymphoproliferative Disorders. Science, 234(4776), 596â601.

Authors 11

S. Zaki Salahuddin (first)
Dharam V. Ablashi (additional)
Phillip D. Markham (additional)
Steven F. Josephs (additional)
Susi Sturzenegger (additional)
Mark Kaplan (additional)
Gregory Halligan (additional)
Peter Biberfeld (additional)
Flossie Wong-Staal (additional)
Bernhard Kramarsky (additional)
Robert C. Gallo (additional)

References 30 Referenced 1,135

ABLASHI, D.V., ONCOGENIC HERPESVIRUSES OF NONHUMAN-PRIMATES, COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES 2: 229 (1979). (10.1016/0147-9571(79)90011-0) / COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES (1979)
ABLASHI, D.V., EPSTEIN BARR VIRUS A: 392 (1985). / EPSTEIN BARR VIRUS A (1985)
ALFORD, C.A., VIROLOGY: 629 (1985). / VIROLOGY (1985)
BIBERFELD P unpublished data.
10.1126/science.2425430
Dahlberg, J. E., Journal of Virology 53: 279 (1985). (10.1128/jvi.53.1.279-286.1985) / Journal of Virology (1985)
GALLO, R. C., CANCER SURVEYS 3: 113 (1984). / CANCER SURVEYS (1984)
10.1126/science.6200936
GELB, L.D., VIRPLOGY: 591 (1985). / VIRPLOGY (1985)
GLEAVES, C.A., COMPARISON OF STANDARD TUBE AND SHELL VIAL CELL-CULTURE TECHNIQUES FOR THE DETECTION OF CYTOMEGALO-VIRUS IN CLINICAL SPECIMENS, JOURNAL OF CLINICAL MICROBIOLOGY 21: 217 (1985). (10.1128/jcm.21.2.217-221.1985) / JOURNAL OF CLINICAL MICROBIOLOGY (1985)
HARPER, M.E., DETECTION OF LYMPHOCYTES EXPRESSING HUMAN LYMPHOTROPIC-T VIRUS TYPE-III IN LYMPH-NODES AND PERIPHERAL-BLOOD FROM INFECTED INDIVIDUALS BY INSITU HYBRIDIZATION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 772 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
HENLE, G, IMMUNOFLUORESCENCE IN CELLS DERIVED FROM BURKITTS LYMPHOMA, JOURNAL OF BACTERIOLOGY 91: 1248 (1966). (10.1128/jb.91.3.1248-1256.1966) / JOURNAL OF BACTERIOLOGY (1966)
JOSEPHS, S.F., GENOMIC ANALYSIS OF THE HUMAN B-LYMPHOTROPIC VIRUS (HBLV), SCIENCE 234: 601 (1986). (10.1126/science.3020691) / SCIENCE (1986)
10.1126/science.6981847
10.1126/science.3006256
KIEFF, E, EPSTEINBARR VIRUS AS: 222 (1985). / EPSTEINBARR VIRUS AS (1985)
MAGRATH, I.T., VIROLOGY 97: 447 (1979). / VIROLOGY (1979)
MILLER, G, VIROLOGY: 497 (1985). / VIROLOGY (1985)
10.1126/science.181845
PEARSON, G.R., EPSTEIN BARR VIRUS A: 411 (1985). / EPSTEIN BARR VIRUS A (1985)
10.1126/science.6200935
RAPP, F, THE CHALLENGE OF HERPESVIRUSES, CANCER RESEARCH 44: 1309 (1984). / CANCER RESEARCH (1984)
REEDMAN, B.M., CELLULAR LOCALIZATION OF AN EPSTEIN-BARR VIRUS (EBV)-ASSOCIATED COMPLEMENT-FIXING ANTIGEN IN PRODUCER AND NON-PRODUCER LYMPHOBLASTOID CELL LINES, INTERNATIONAL JOURNAL OF CANCER 11: 499 (1973). (10.1002/ijc.2910110302) / INTERNATIONAL JOURNAL OF CANCER (1973)
ROIZMAN, B, VIROLOGY: 497 (1985). / VIROLOGY (1985)
RUSCETTI, F.W., J IMMUNOL 119: 113 (1977). / J IMMUNOL (1977)
SALAHUDDIN S.Z. unpublished data.
SHUSTER, E.A., MONOCLONAL-ANTIBODY FOR RAPID LABORATORY DETECTION OF CYTOMEGALO-VIRUS INFECTIONS - CHARACTERIZATION AND DIAGNOSTIC APPLICATION, MAYO CLINIC PROCEEDINGS 60: 577 (1985). (10.1016/S0025-6196(12)60979-3) / MAYO CLINIC PROCEEDINGS (1985)
10.1126/science.6189183
SMITH, J.D., HERPES-SIMPLEX VIRUS AND HUMAN CYTOMEGALOVIRUS REPLICATION IN WI-38 CELLS .1. SEQUENCE OF VIRAL REPLICATION, JOURNAL OF VIROLOGY 12: 919 (1973). (10.1128/jvi.12.4.919-930.1973) / JOURNAL OF VIROLOGY (1973)
10.1038/317395a0

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:31 a.m.)
Indexed	5 days, 8 hours ago (Aug. 28, 2025, 8:50 a.m.)
Issued	38 years, 10 months ago (Oct. 31, 1986)
Published	38 years, 10 months ago (Oct. 31, 1986)
Published Print	38 years, 10 months ago (Oct. 31, 1986)

Funders 0

None

BibTeX

@article{Salahuddin_1986, title={Isolation of a New Virus, HBLV, in Patients with Lymphoproliferative Disorders}, volume={234}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2876520}, DOI={10.1126/science.2876520}, number={4776}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Salahuddin, S. Zaki and Ablashi, Dharam V. and Markham, Phillip D. and Josephs, Steven F. and Sturzenegger, Susi and Kaplan, Mark and Halligan, Gregory and Biberfeld, Peter and Wong-Staal, Flossie and Kramarsky, Bernhard and Gallo, Robert C.}, year={1986}, month=oct, pages={596–601} }

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