DOI: 10.1126/science.2865810. A Glycophospholipid Tail at the Carboxyl Terminus of the Thy-1 Glycoprotein of Neurons and Thymocytes

A Glycophospholipid Tail at the Carboxyl Terminus of the Thy-1 Glycoprotein of Neurons and Thymocytes

10.1126/science.2865810

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Cell surface molecules of eukaryotic cells have been considered to be integrated into the membrane bilayer by a transmembrane protein sequence. The Thy-1 antigen of rodent thymocytes and brain was the first eukaryotic membrane molecule for which biochemical data clearly suggested membrane integration via a nonprotein tail. Direct evidence is now presented showing that a glycophospholipid structure is attached to the carboxyl-terminal cysteine residue and that 31 carboxyl-terminal amino acids predicted from the Thy-1 complementary DNA sequence are not present in the mature glycoprotein. These experimental results raise questions concerning signaling across a cell membrane since antibodies to Thy-1 can stimulate T lymphocytes to release lymphokines and undergo cell division.

Bibliography

Tse, A. G. D., Barclay, A. N., Watts, A., & Williams, A. F. (1985). A Glycophospholipid Tail at the Carboxyl Terminus of the Thy-1 Glycoprotein of Neurons and Thymocytes. Science, 230(4729), 1003â1008.

Authors 4

Albert G. D. Tse (first)
A. Neil Barclay (additional)
Anthony Watts (additional)
Alan F. Williams (additional)

References 46 Referenced 218

ALEXANDER, H, MIMICKING THE ALLOANTIGENICITY OF PROTEINS WITH CHEMICALLY SYNTHESIZED PEPTIDES DIFFERING IN SINGLE AMINO-ACIDS, NATURE 306: 697 (1983). (10.1038/306697a0) / NATURE (1983)
ALLEN, A.K., QUANTITATION OF GLUCOSAMINE AND GALACTOSAMINE IN GLYCOPROTEINS AFTER HYDROLYSIS IN PARA-TOLUENESULFONIC ACID, FEBS LETTERS 60: 76 (1975). (10.1016/0014-5793(75)80422-4) / FEBS LETTERS (1975)
ARAKAWA, Y, DETERMINATION OF NEUTRAL AND AMINO-SUGARS IN GLYCOPROTEINS BY GAS-CHROMATOGRAPHY, CHEMICAL & PHARMACEUTICAL BULLETIN 24: 2032 (1976). (10.1248/cpb.24.2032) / CHEMICAL & PHARMACEUTICAL BULLETIN (1976)
10.1073/pnas.82.10.3207
BARCLAY, A.N., CHEMICAL CHARACTERIZATION OF THY-1 GLYCOPROTEINS FROM MEMBRANES OF RAT THYMOCYTES AND BRAIN, NATURE 263: 563 (1976). (10.1038/263563a0) / NATURE (1976)
BEECH, J.N., DENSITY OF THY-1 ON AXONAL MEMBRANE OF DIFFERENT RAT NERVES, JOURNAL OF NEUROCHEMISTRY 41: 411 (1983). (10.1111/j.1471-4159.1983.tb04757.x) / JOURNAL OF NEUROCHEMISTRY (1983)
BOOTHROYD, J.C., VARIANT SURFACE GLYCOPROTEINS OF TRYPANOSOMA-BRUCEI ARE SYNTHESIZED WITH CLEAVABLE HYDROPHOBIC SEQUENCES AT THE CARBOXY AND AMINO TERMINI, NUCLEIC ACIDS RESEARCH 9: 4735 (1981). (10.1093/nar/9.18.4735) / NUCLEIC ACIDS RESEARCH (1981)
BROWN, WRA, LYMPHOCYTE CELL-SURFACE GLYCOPROTEINS WHICH BIND TO SOYBEAN AND PEANUT LECTINS, IMMUNOLOGY 46: 713 (1982). / IMMUNOLOGY (1982)
CAMPBELL, D.G., RAT-BRAIN THY-1 GLYCOPROTEIN - THE AMINO-ACID-SEQUENCE, DISULFIDE BONDS AND AN UNUSUAL HYDROPHOBIC REGION, BIOCHEMICAL JOURNAL 195: 15 (1981). (10.1042/bj1950015) / BIOCHEMICAL JOURNAL (1981)
CARLSSON, S.R., PURIFICATION AND CHARACTERIZATION OF THE MOUSE THYMOCYTE THY-1 GLYCOPROTEIN, BIOCHEMICAL JOURNAL 211: 641 (1983). (10.1042/bj2110641) / BIOCHEMICAL JOURNAL (1983)
CHAPLIN, M.F., A RAPID AND SENSITIVE METHOD FOR THE ANALYSIS OF CARBOHYDRATE COMPONENTS IN GLYCOPROTEINS USING GAS-LIQUID-CHROMATOGRAPHY, ANALYTICAL BIOCHEMISTRY 123: 336 (1982). (10.1016/0003-2697(82)90455-9) / ANALYTICAL BIOCHEMISTRY (1982)
COHEN, F.E., ANALYSIS OF STRUCTURAL SIMILARITIES BETWEEN BRAIN THY-1 ANTIGEN AND IMMUNOGLOBULIN DOMAINS - EVIDENCE FOR AN EVOLUTIONARY RELATIONSHIP AND A HYPOTHESIS FOR ITS FUNCTIONAL-SIGNIFICANCE, BIOCHEMICAL JOURNAL 195: 31 (1981). (10.1042/bj1950031) / BIOCHEMICAL JOURNAL (1981)
COTMORE, S.F., PURIFICATION OF THY-1-RELATED GLYCOPROTEINS FROM HUMAN-BRAIN AND FIBROBLASTS - COMPARISONS BETWEEN THESE MOLECULES AND MURINE GLYCOPROTEINS CARRYING THY-1.1 AND THY-1.2 ANTIGENS, EUROPEAN JOURNAL OF IMMUNOLOGY 11: 597 (1981). (10.1002/eji.1830110802) / EUROPEAN JOURNAL OF IMMUNOLOGY (1981)
EIBL, H, A NEW, SENSITIVE DETERMINATION OF PHOSPHATE, ANALYTICAL BIOCHEMISTRY 30: 51 (1969). (10.1016/0003-2697(69)90372-8) / ANALYTICAL BIOCHEMISTRY (1969)
Ferguson, M. A., Journal of Biological Chemistry 260: 14547 (1985). (10.1016/S0021-9258(17)38603-9) / Journal of Biological Chemistry (1985)
FERGUSON, MAJ, TRYPANOSOMA-BRUCEI VARIANT SURFACE GLYCOPROTEIN HAS A SN-1,2-DIMYRISTYL GLYCEROL MEMBRANE ANCHOR AT ITS COOH TERMINUS, JOURNAL OF BIOLOGICAL CHEMISTRY 260: 4963 (1985). (10.1016/S0021-9258(18)89166-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1985)
FUTERMAN, A.H., PHYSICOCHEMICAL BEHAVIOR AND STRUCTURAL CHARACTERISTICS OF MEMBRANE-BOUND ACETYLCHOLINESTERASE FROM TORPEDO ELECTRIC ORGAN - EFFECT OF PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C, BIOCHEMICAL JOURNAL 226: 369 (1985). (10.1042/bj2260369) / BIOCHEMICAL JOURNAL (1985)
GLASS, R.L., ALCOHOLYSIS, SAPONIFICATION AND PREPARATION OF FATTY-ACID METHYL-ESTERS, LIPIDS 6: 919 (1971). (10.1007/BF02531175) / LIPIDS (1971)
GUNTER, K.C., T-CELL-ACTIVATING PROPERTIES OF AN ANTI-THY-1 MONOCLONAL-ANTIBODY - POSSIBLE ANALOGY TO OKT3/LEU-4, JOURNAL OF EXPERIMENTAL MEDICINE 159: 716 (1984). (10.1084/jem.159.3.716) / JOURNAL OF EXPERIMENTAL MEDICINE (1984)
HANTKE, K, COVALENT BINDING OF LIPID TO PROTEIN - DIGLYCERIDE AND AMIDE-LINKED FATTY-ACID AT N-TERMINAL END OF MUREIN-LIPOPROTEIN OF ESCHERICHIA-COLI OUTER MEMBRANE, EUROPEAN JOURNAL OF BIOCHEMISTRY 34: 284 (1973). (10.1111/j.1432-1033.1973.tb02757.x) / EUROPEAN JOURNAL OF BIOCHEMISTRY (1973)
Henderson, L. E., Proceedings of the National Academy of Sciences of the United States of America 80: 339 (1983). / Proceedings of the National Academy of Sciences of the United States of America (1983)
HORNING, M.G., ANALYTICAL LETTERS 1: 311 (1968). (10.1080/00032716808051134) / ANALYTICAL LETTERS (1968)
KAMIYA, Y, STRUCTURE OF RHODOTORUCINE-A, A PEPTIDYL FACTOR, INDUCING MATING TUBE FORMATION IN RHODOSPORIDIUM-TORULOIDES, AGRICULTURAL AND BIOLOGICAL CHEMISTRY 43: 363 (1979). / AGRICULTURAL AND BIOLOGICAL CHEMISTRY (1979)
KAUFMAN, J.F., CYSTEINES IN THE TRANSMEMBRANE REGION OF MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGENS ARE FATTY ACYLATED VIA THIOESTER BONDS, JOURNAL OF BIOLOGICAL CHEMISTRY 259: 7230 (1984). (10.1016/S0021-9258(17)39862-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1984)
KUCHEL, P.W., MOLECULAR-WEIGHTS OF THY-1 GLYCOPROTEINS FROM RAT THYMUS AND BRAIN IN PRESENCE AND ABSENCE OF DEOXYCHOLATE, BIOCHEMICAL JOURNAL 169: 411 (1978). (10.1042/bj1690411) / BIOCHEMICAL JOURNAL (1978)
LAI, J.S., BACILLUS-LICHENIFORMIS PENICILLINASE SYNTHESIZED IN ESCHERICHIA-COLI CONTAINS COVALENTLY LINKED FATTY-ACID AND GLYCERIDE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 78: 3506 (1981). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1981)
LEBARON, F.W., MICROSPECTROPHOTOMETRIC DETERMINATION OF INOSITOL IN TISSUES AND LIPIDE HYDROLYSATES, FEDERATION PROCEEDINGS 16: 209 (1957). / FEDERATION PROCEEDINGS (1957)
10.1126/science.6143400
LETARTE, M, PURIFICATION AND CHARACTERIZATION OF MOUSE-BRAIN THY-1 GLYCOPROTEIN ANTIGENS, JOURNAL OF IMMUNOLOGY 121: 1718 (1978). (10.4049/jimmunol.121.5.1718) / JOURNAL OF IMMUNOLOGY (1978)
LOW, M.G., ROLE OF PHOSPHATIDYLINOSITOL IN ATTACHMENT OF ALKALINE-PHOSPHATASE TO MEMBRANES, BIOCHEMISTRY 19: 3913 (1980). (10.1021/bi00558a004) / BIOCHEMISTRY (1980)
Low, M. G., Nature 318:62 (1985). (10.1038/318062a0) / Nature (1985)
LUESCHER, B, BIOSYNTHESIS OF MOUSE THY-1 ANTIGEN, JOURNAL OF IMMUNOLOGY 134: 1084 (1985). (10.4049/jimmunol.134.2.1084) / JOURNAL OF IMMUNOLOGY (1985)
MACDONALD, H.R., PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTI-THY-1 ANTIBODIES THAT STIMULATE LYMPHOKINE PRODUCTION BY CYTOLYTIC T-CELL CLONES, EUROPEAN JOURNAL OF IMMUNOLOGY 15: 495 (1985). (10.1002/eji.1830150514) / EUROPEAN JOURNAL OF IMMUNOLOGY (1985)
MAINO, V.C., MECHANISM OF THY-1-MEDIATED T-CELL ACTIVATION - ROLES OF FC-RECEPTORS, T200, IA, AND H-2 GLYCOPROTEINS IN ACCESSORY CELL-FUNCTION, JOURNAL OF IMMUNOLOGY 126: 1829 (1981). (10.4049/jimmunol.126.5.1829) / JOURNAL OF IMMUNOLOGY (1981)
MCCLAIN, L.D., PURIFICATION AND CHARACTERIZATION OF MOUSE-BRAIN THY-1.2 DIFFERENTIATION ALLOANTIGEN, BRAIN RESEARCH 159: 161 (1978). (10.1016/0006-8993(78)90117-8) / BRAIN RESEARCH (1978)
METCALFE, L.D., RAPID PREPARATION OF FATTY ACID ESTERS FOR GAS CHROMATOGRAPHIC ANALYSIS, ANALYTICAL CHEMISTRY 33: 363 (1961). (10.1021/ac60171a016) / ANALYTICAL CHEMISTRY (1961)
OMARY, M.B., COVALENT BINDING OF FATTY-ACID TO THE TRANSFERRIN RECEPTOR IN CULTURED HUMAN-CELLS, JOURNAL OF BIOLOGICAL CHEMISTRY 256: 4715 (1981). (10.1016/S0021-9258(19)69309-9) / JOURNAL OF BIOLOGICAL CHEMISTRY (1981)
OWEN, M.J., LABELING OF LYMPHOCYTE SURFACE-ANTIGENS BY THE LIPOPHILIC, PHOTOACTIVATABLE REAGENT HEXANOYLDIIODO-N-(4-AZIDO-2-NITROPHENYL)TYRAMINE, BIOCHEMISTRY 19: 3092 (1980). (10.1021/bi00554a040) / BIOCHEMISTRY (1980)
PELLMAN, D, AN N-TERMINAL PEPTIDE FROM P60SRC CAN DIRECT MYRISTYLATION AND PLASMA-MEMBRANE LOCALIZATION WHEN FUSED TO HETEROLOGOUS PROTEINS, NATURE 314: 374 (1985). (10.1038/314374a0) / NATURE (1985)
PONT, S, CLONOSPECIFIC STRUCTURAL HETEROGENEITY IN THE THY-1 MOLECULE FROM MOUSE LYMPHOCYTES-T, IMMUNOGENETICS 21: 459 (1985). (10.1007/BF00430929) / IMMUNOGENETICS (1985)
10.1084/jem.120.3.413
ROUSER, G, QUANTITATIVE LIQUID COLUMN AND THIN-LAYER CHROMATOGRAPHY OF LIPIDS AND OTHER WATER INSOLUBLE SUBSTANCES, ELUTION SELECTIVITY PRINCIPLES, AND A GRAPHIC METHOD FOR PATTERN ANALYSIS OF CHROMATOGRAPHIC DATA, JOURNAL OF CHROMATOGRAPHIC SCIENCE 11: 60 (1973). (10.1093/chromsci/11.2.60) / JOURNAL OF CHROMATOGRAPHIC SCIENCE (1973)
SCHMIDT, MFG, FATTY-ACID BINDING TO VESICULAR STOMATITIS-VIRUS GLYCOPROTEIN - NEW TYPE OF POST-TRANSLATIONAL MODIFICATION OF THE VIRAL GLYCOPROTEIN, CELL 17: 813 (1979). (10.1016/0092-8674(79)90321-0) / CELL (1979)
SEKI, T, STRUCTURAL ORGANIZATION OF THE RAT THY-1 GENE, NATURE 313: 485 (1985). (10.1038/313485a0) / NATURE (1985)
10.1126/science.2857501
10.1126/science.6177036

Dates

Type	When
Created	18 years, 10 months ago (Oct. 5, 2006, 3:42 p.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:06 a.m.)
Indexed	1 year, 3 months ago (May 10, 2024, 4:53 a.m.)
Issued	39 years, 9 months ago (Nov. 29, 1985)
Published	39 years, 9 months ago (Nov. 29, 1985)
Published Print	39 years, 9 months ago (Nov. 29, 1985)

Funders 0

None

BibTeX

@article{Tse_1985, title={A Glycophospholipid Tail at the Carboxyl Terminus of the Thy-1 Glycoprotein of Neurons and Thymocytes}, volume={230}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2865810}, DOI={10.1126/science.2865810}, number={4729}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Tse, Albert G. D. and Barclay, A. Neil and Watts, Anthony and Williams, Alan F.}, year={1985}, month=nov, pages={1003–1008} }

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      "DOI": "10.1016/S0021-9258(17)39862-9",
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      "DOI": "10.1042/bj1690411",
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      "journal-title": "BIOCHEMICAL JOURNAL"
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      "DOI": "10.1002/eji.1830150514",
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      "DOI": "10.1016/S0021-9258(19)69309-9",
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      "first-page": "374",
      "DOI": "10.1038/314374a0",
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