Crystal Structure of Thermotoga maritima Ribosome Recycling Factor: A tRNA Mimic
10.1126/science.286.5448.2349
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Ribosome recycling factor (RRF), together with elongation factor G (EF-G), catalyzes recycling of ribosomes after one round of protein synthesis. The crystal structure of RRF was determined at 2.55 angstrom resolution. The protein has an unusual fold where domain I is a long three-helix bundle and domain II is a three-layer β/α/β sandwich. The molecule superimposes almost perfectly with a transfer RNA (tRNA) except that the amino acid–binding 3′ end is missing. The mimicry suggests that RRF interacts with the posttermination ribosomal complex in a similar manner to a tRNA, leading to disassembly of the complex. The structural arrangement of this mimicry is entirely different from that of other cases of less pronounced mimicry of tRNA so far described.

Bibliography

Selmer, M., Al-Karadaghi, S., Hirokawa, G., Kaji, A., & Liljas, A. (1999). Crystal Structure of Thermotoga maritima Ribosome Recycling Factor: A tRNA Mimic. Science, 286(5448), 2349–2352.

Authors 5 University of Pennsylvania
  1. Maria Selmer (first)
  2. Salam Al-Karadaghi (additional)
  3. Go Hirokawa (additional) University of Pennsylvania
  4. Akira Kaji (additional) University of Pennsylvania
  5. Anders Liljas (additional)
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Dates
Type When
Created 23 years ago (July 27, 2002, 5:42 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 5:35 a.m.)
Indexed 1 month, 1 week ago (July 11, 2025, 6:51 a.m.)
Issued 25 years, 8 months ago (Dec. 17, 1999)
Published 25 years, 8 months ago (Dec. 17, 1999)
Published Print 25 years, 8 months ago (Dec. 17, 1999)
Funders 0

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@article{Selmer_1999, title={Crystal Structure of Thermotoga maritima Ribosome Recycling Factor: A tRNA Mimic}, volume={286}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.286.5448.2349}, DOI={10.1126/science.286.5448.2349}, number={5448}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Selmer, Maria and Al-Karadaghi, Salam and Hirokawa, Go and Kaji, Akira and Liljas, Anders}, year={1999}, month=dec, pages={2349–2352} }