DOI: 10.1126/science.286.5447.2153. Three-Dimensional Structure of the Human TFIID-IIA-IIB Complex

Three-Dimensional Structure of the Human TFIID-IIA-IIB Complex

10.1126/science.286.5447.2153

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The multisubunit transcription factor IID (TFIID) is an essential component of the eukaryotic RNA polymerase II machinery that works in concert with TFIIA (IIA) and TFIIB (IIB) to assemble initiation complexes at core eukaryotic promoters. Here the structures of human TFIID and the TFIID-IIA-IIB complex that were obtained by electron microscopy and image analysis to 35 angstrom resolution are presented. TFIID is a trilobed, horseshoe-shaped structure, with TFIIA and TFIIB bound on opposite lobes and flanking a central cavity. Antibody studies locate the TATA-binding protein (TBP) between TFIIA and TFIIB at the top of the cavity that most likely encompasses the TATA DNA binding region of the supramolecular complex.

Bibliography

Andel, F., Ladurner, A. G., Inouye, C., Tjian, R., & Nogales, E. (1999). Three-Dimensional Structure of the Human TFIID-IIA-IIB Complex. Science, 286(5447), 2153â2156.

Authors 5

Frank Andel (first)
Andreas G. Ladurner (additional)
Carla Inouye (additional)
Robert Tjian (additional)
Eva Nogales (additional)

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F. Andel III A. G. Ladurner C. Inouye R. Tjian E. Nogales data not shown.
We thank R. Glaeser W. Nicholson and K. Downing for helpful discussions and O. Arum for help in scanning micrographs. Supported by grants from the NIH General Medical Sciences (E.N.) the NIH Cancer Institute (R.T.) and the U.S. Department of Energy in contract with the University of California (E.N.).

Dates

Type	When
Created	23 years ago (July 27, 2002, 5:42 a.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 5:32 a.m.)
Indexed	3 weeks, 1 day ago (July 30, 2025, 10:06 a.m.)
Issued	25 years, 8 months ago (Dec. 10, 1999)
Published	25 years, 8 months ago (Dec. 10, 1999)
Published Print	25 years, 8 months ago (Dec. 10, 1999)

Funders 0

None

BibTeX

@article{Andel_1999, title={Three-Dimensional Structure of the Human TFIID-IIA-IIB Complex}, volume={286}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.286.5447.2153}, DOI={10.1126/science.286.5447.2153}, number={5447}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Andel, Frank and Ladurner, Andreas G. and Inouye, Carla and Tjian, Robert and Nogales, Eva}, year={1999}, month=dec, pages={2153–2156} }

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