DOI: 10.1126/science.286.5446.1960. A Pair of Related Genes with Antagonistic Roles in Mediating Flowering Signals

10.1126/science.286.5446.1960

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Flowering in Arabidopsis is promoted via several interacting pathways. A photoperiod-dependent pathway relays signals from photoreceptors to a transcription factor gene, CONSTANS ( CO ), which activates downstream meristem identity genes such as LEAFY ( LFY ). FT , together with LFY , promotes flowering and is positively regulated by CO . Loss of FT causes delay in flowering, whereas overexpression of FT results in precocious flowering independent of CO or photoperiod. FT acts in part downstream of CO and mediates signals for flowering in an antagonistic manner with its homologous gene, TERMINAL FLOWER1 ( TFL1 ).

Bibliography

Kobayashi, Y., Kaya, H., Goto, K., Iwabuchi, M., & Araki, T. (1999). A Pair of Related Genes with Antagonistic Roles in Mediating Flowering Signals. Science, 286(5446), 1960â1962.

Authors 5

Yasushi Kobayashi (first)
Hidetaka Kaya (additional)
Koji Goto (additional)
Masaki Iwabuchi (additional)
Takashi Araki (additional)

References 31 Referenced 1,067

10.1105/tpc.10.12.1973
10.1146/annurev.arplant.49.1.345
Ruiz-Garcı́a L., et al., Plant Cell 9, 1921 (1997). / Plant Cell by Ruiz-Garcı́a L. (1997)
A T-DNA insertion line (vTAAT26C51) with a 75.8-kb deletion in the FT-FAS1 region was identified. The bacterial artificial chromosome (BAC) clone F5I14 (GenBank accession number ) was found to cover the deleted region. One candidate gene ( F5I14.3 ) with similarity to TFL1 (4) was examined in six ft alleles including three new ones ( ft-4 ft-5 and ft-6 from ecotype Nossen) [this study and (16)] and a nucleotide substitution was found in all cases. The entire F5I14.3 was deleted in vTAAT26C51. On the basis of these results we concluded that F5I14.3 is the FT gene. Sequences of cDNA were deposited in GenBank (accession numbers and ).
10.1126/science.275.5296.80
10.1007/s004380050407
Other members are: TWIN SISTER OF FT ( TSF GenBank accession number ) ARABIDOPSIS THALIANA CENTRORADIALIS ( ATC GenBank accession number ) BROTHER OF FT AND TFL1 ( BFT = MTG10.5 GenBank accession number ) and MOTHER OF FT AND TFL1 ( MFT GenBank accession number ). The nomenclature was decided through agreement between D. Weigel's group and ours.
The putative orthologs are a Citrus unshiu expressed sequence tag clone for CiFT (GenBank accession number ) and a rice BAC clone nbxb0035E07r (GenBank accession number ) containing a part of OsFT.
The period of floral commitment was determined by expression of pAP1::GUS and pAP3::GUS reporter genes.
10.1016/0092-8674(95)90288-0
10.1038/384059a0
10.1126/science.279.5355.1360
10.1016/S0092-8674(00)80256-1
10.1038/377495a0
10.1038/377522a0
For additional data see Science Online (www.sciencemag.org/feature/data/1044707.shl).
M. I. Igeño and G. Coupland personal communication.
10.1007/BF00264213
10.2307/3869152
10.1111/j.1365-313X.1992.00103.x
10.1242/dev.125.9.1609
Y. Kobayashi and T. Araki unpublished data.
10.1105/tpc.11.6.1007
10.1016/0014-5793(95)00376-K
10.1016/S0969-2126(98)00125-7
10.1016/S0969-2126(98)00126-9
10.1016/0169-328X(95)00029-R
Single-letter abbreviations for amino acid residues are as follows: A Ala; C Cys; D Asp; E Glu; F Phe; G Gly; H His; I Ile; K Lys; L Leu; M Met; N Asn; P Pro; Q Gln; R Arg; S Ser; T Thr; V Val; W Trp; and Y Tyr.
J. Kyozuka personal communication.
35S::CO:GR; co-2 plants were grown on MS medium (3% sucrose 0.8% agar) under LD (16 hours light/8 hours dark) conditions. On day 14 5 ml of 10 μM dex solution was applied to the medium (25 ml) to give a final concentration of 1.7 μM.
We particularly thank D. Weigel for exchange of unpublished results and materials. We thank G. Coupland and Plant Bioscience Limited M. Koornneef M. Omura M. Yanofsky and the NSF-supported Arabidopsis Biological Resource Center for materials and G. Coupland M. Koornneef and J. Kyozuka for communicating unpublished results. Supported by grants from the Ministry of Education Science Culture and Sports of Japan and an RFTF grant from the Japan Society for the Promotion of Science (JSPS). Y.K. is a JSPS Research Fellow.

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:42 a.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 4:30 a.m.)
Indexed	5 days, 5 hours ago (Aug. 23, 2025, 12:59 a.m.)
Issued	25 years, 8 months ago (Dec. 3, 1999)
Published	25 years, 8 months ago (Dec. 3, 1999)
Published Print	25 years, 8 months ago (Dec. 3, 1999)

Funders 0

None

BibTeX

@article{Kobayashi_1999, title={A Pair of Related Genes with Antagonistic Roles in Mediating Flowering Signals}, volume={286}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.286.5446.1960}, DOI={10.1126/science.286.5446.1960}, number={5446}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kobayashi, Yasushi and Kaya, Hidetaka and Goto, Koji and Iwabuchi, Masaki and Araki, Takashi}, year={1999}, month=dec, pages={1960–1962} }

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