Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans
10.1126/science.285.5433.1558
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

A whole-genome restriction map of Deinococcus radiodurans , a radiation-resistant bacterium able to survive up to 15,000 grays of ionizing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electrophoresis. Very large, randomly sheared, genomic DNA fragments were used to construct maps from individual DNA molecules that were assembled into two circular overlapping maps (2.6 and 0.415 megabases), without gaps. A third smaller chromosome (176 kilobases) was identified and characterized. Aberrant nonlinear DNA structures that may define chromosome structure and organization, as well as intermediates in DNA repair, were directly visualized by optical mapping techniques after γ irradiation.

Bibliography

Lin, J., Qi, R., Aston, C., Jing, J., Anantharaman, T. S., Mishra, B., White, O., Daly, M. J., Minton, K. W., Venter, J. C., & Schwartz, D. C. (1999). Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans. Science, 285(5433), 1558–1562.

Authors 11
  1. Jieyi Lin (first)
  2. Rong Qi (additional)
  3. Christopher Aston (additional)
  4. Junping Jing (additional)
  5. Thomas S. Anantharaman (additional)
  6. Bud Mishra (additional)
  7. Owen White (additional)
  8. Michael J. Daly (additional)
  9. Kenneth W. Minton (additional)
  10. J. Craig Venter (additional)
  11. David C. Schwartz (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:37 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 5:06 a.m.)
Indexed 2 months ago (July 2, 2025, 12:35 p.m.)
Issued 26 years ago (Sept. 3, 1999)
Published 26 years ago (Sept. 3, 1999)
Published Print 26 years ago (Sept. 3, 1999)
Funders 0

None

@article{Lin_1999, title={Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans}, volume={285}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.285.5433.1558}, DOI={10.1126/science.285.5433.1558}, number={5433}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Lin, Jieyi and Qi, Rong and Aston, Christopher and Jing, Junping and Anantharaman, Thomas S. and Mishra, Bud and White, Owen and Daly, Michael J. and Minton, Kenneth W. and Venter, J. Craig and Schwartz, David C.}, year={1999}, month=sep, pages={1558–1562} }