Abstract
The α and β subunits of the γ-aminobutyric acidA (GABA A ) receptor were expressed individually in Xenopus oocytes by injection of RNA synthesized from their cloned DNAs. GABA-sensitive chloride channels were detected several days after injection with any one of three different α RNAs (α 1 , α 2 , and α 3 ) or with β RNA. The channels induced by each of the α-subunit RNAs were indistinguishable, they had multiple conductance levels (10, 19, 28, and 42 picosiemens), and their activity was potentiated by pentobarbital and inhibited by picrotoxin. The β channels usually expressed poorly but showed similar single channel conductance levels (10, 18, 27, and 40 picosiemens), potentiation by pentobarbital and inhibition by picrotoxin. The finding that both α and β subunits, examined separately, form GABA-sensitive ion channels with permeation properties and regulatory sites characteristic of the native receptor suggests that the amino acid sequences that confer these properties are within the homologous domains shared by the subunits.
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Dates
| Type | When |
|---|---|
| Created | 18 years, 10 months ago (Oct. 5, 2006, 5:21 p.m.) |
| Deposited | 1 year, 7 months ago (Jan. 13, 2024, 5:18 a.m.) |
| Indexed | 1 month, 3 weeks ago (July 2, 2025, 3:31 a.m.) |
| Issued | 36 years, 10 months ago (Oct. 28, 1988) |
| Published | 36 years, 10 months ago (Oct. 28, 1988) |
| Published Print | 36 years, 10 months ago (Oct. 28, 1988) |
@article{Blair_1988, title={Single Subunits of the GABA A Receptor Form Ion Channels with Properties of the Native Receptor}, volume={242}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2845583}, DOI={10.1126/science.2845583}, number={4878}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Blair, Leslie A. C. and Levitan, Edwin S. and Marshall, John and Dionne, Vincent E. and Barnard, Eric A.}, year={1988}, month=oct, pages={577–579} }