DOI: 10.1126/science.284.5423.2174. Regulation of Transcription by a Protein Methyltransferase

Regulation of Transcription by a Protein Methyltransferase

10.1126/science.284.5423.2174

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

The p160 family of coactivators, SRC-1, GRIP1/TIF2, and p/CIP, mediate transcriptional activation by nuclear hormone receptors. Coactivator-associated arginine methyltransferase 1 (CARM1), a previously unidentified protein that binds to the carboxyl-terminal region of p160 coactivators, enhanced transcriptional activation by nuclear receptors, but only when GRIP1 or SRC-1a was coexpressed. Thus, CARM1 functions as a secondary coactivator through its association with p160 coactivators. CARM1 can methylate histone H3 in vitro, and a mutation in the putativeS-adenosylmethionine binding domain of CARM1 substantially reduced both methyltransferase and coactivator activities. Thus, coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.

Bibliography

Chen, D., Ma, H., Hong, H., Koh, S. S., Huang, S.-M., Schurter, B. T., Aswad, D. W., & Stallcup, M. R. (1999). Regulation of Transcription by a Protein Methyltransferase. Science, 284(5423), 2174â2177.

Authors 8

Dagang Chen (first)
Han Ma (additional)
Heng Hong (additional)
Stephen S. Koh (additional)
Shih-Ming Huang (additional)
Brandon T. Schurter (additional)
Dana W. Aswad (additional)
Michael R. Stallcup (additional)

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We thank the following investigators for providing cDNA clones: H. R. Herschman (University of California Los Angeles) for PRMT1 R. M. Evans (Salk institute La Jolla CA) for ACTR M.-J. Tsai and B. W. O'Malley (Baylor College of Medicine Houston TX) for SRC-1a and A. O. Brinkmann (Erasmus University Rotterdam Netherlands) for androgen receptor. We thank S. Subramanian L. Yang and R. Widelitz for technical assistance and D. Johnson and R. Maxson (University of Southern California) for critical comments on the manuscript. Supported by USPHS grants DK43093 (M.R.S.) and NS17269 (D.W.A.) from the NIH and by a predoctoral traineeship from the University of California Breast Cancer Research Program (H.M.) and a predoctoral traineeship from grant AG00093 (S.S.K.) from the National Institutes of Health.

Dates

Type	When
Created	23 years ago (July 27, 2002, 5:42 a.m.)
Deposited	8 months, 2 weeks ago (Dec. 8, 2024, 1:10 p.m.)
Indexed	2 weeks, 3 days ago (Aug. 5, 2025, 9 a.m.)
Issued	26 years, 1 month ago (June 25, 1999)
Published	26 years, 1 month ago (June 25, 1999)
Published Print	26 years, 1 month ago (June 25, 1999)

Funders 0

None

BibTeX

@article{Chen_1999, title={Regulation of Transcription by a Protein Methyltransferase}, volume={284}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.284.5423.2174}, DOI={10.1126/science.284.5423.2174}, number={5423}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Chen, Dagang and Ma, Han and Hong, Heng and Koh, Stephen S. and Huang, Shih-Ming and Schurter, Brandon T. and Aswad, Dana W. and Stallcup, Michael R.}, year={1999}, month=jun, pages={2174–2177} }

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  "abstract": "<jats:p>The p160 family of coactivators, SRC-1, GRIP1/TIF2, and p/CIP, mediate transcriptional activation by nuclear hormone receptors. Coactivator-associated arginine methyltransferase 1 (CARM1), a previously unidentified protein that binds to the carboxyl-terminal region of p160 coactivators, enhanced transcriptional activation by nuclear receptors, but only when GRIP1 or SRC-1a was coexpressed. Thus, CARM1 functions as a secondary coactivator through its association with p160 coactivators. CARM1 can methylate histone H3 in vitro, and a mutation in the putative<jats:italic>S</jats:italic>-adenosylmethionine binding domain of CARM1 substantially reduced both methyltransferase and coactivator activities. Thus, coactivator-mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.</jats:p>",
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      "unstructured": "Plasmid construction. Mammalian cell expression vectors: pSG5.HA was constructed by inserting a synthetic sequence coding for a translation start signal hemagglutinin A (HA) tag Eco RI site and Xho I site into the Eco RI\u2013Bam HI site of pSG5 (Stratagene) which has SV40 and T7 promoters. The original Eco RI site was destroyed by this insertion but the Bam HI site was preserved leaving a multiple cloning site after the HA tag containing Eco RI Xho I Bam HI and Bgl II sites. The following protein-coding regions were cloned into pSG5.HA in frame with the HA tag with the indicated insertion sites: GRIP1 5\u20131462 (full length) and CARM1 3\u2013608 (full length) at the Eco RI site; GRIP1 5\u2013765 at the Eco RI\u2013Xho I site; GRIP1 730\u20131121 and GRIP1 1121\u20131462 were Eco RI\u2013Sal I fragments inserted at the Eco RI\u2013Xho I site; SRC-1a 1\u20131441 (full length) was a Sma I\u2013Sal I fragment inserted at the Eco RI site which was blunted by filling with Klenow polymerase and the Xho I site. Expression vectors for Gal4DBD fused to various GRIP1 fragments were constructed by inserting the appropriate fragments into pM (Clontech) as follows: GRIP1 1122\u20131462 Eco RI\u2013Bgl II fragment inserted into Eco RI\u2013Bam HI site; GRIP1 563\u20131121 and GRIP1 5\u2013765 Eco RI\u2013Sal I fragments inserted into homologous site. Vectors for GST fusion proteins were constructed in pGEX-4T1 (Pharmacia): for GST-CARM1 the original 3.2-kb Eco RI fragment from pGAD10.CARM1 was inserted; for GST-GRIP1 C (amino acids 1122 to 1462) an Eco RI\u2013Sal I fragment was inserted. The CARM1 VLD-to-AAA mutation was engineered with the Promega Gene Editor Kit."
    },
    {
      "key": "e_1_3_1_25_2",
      "doi-asserted-by": "crossref",
      "first-page": "132",
      "DOI": "10.1016/0003-2697(79)90716-4",
      "volume": "98",
      "author": "Chamberlin J. P.",
      "year": "1978",
      "unstructured": "Chamberlin J. P., Anal. Biochem. 98, 132 (1978).",
      "journal-title": "Anal. Biochem."
    },
    {
      "key": "e_1_3_1_26_2",
      "doi-asserted-by": "crossref",
      "unstructured": "A. O. Brinkmann et al. J. Steroid Biochem. Mol. Biol. 34 307 (1989).",
      "DOI": "10.1016/0022-4731(89)90098-8"
    },
    {
      "key": "e_1_3_1_27_2",
      "doi-asserted-by": "crossref",
      "first-page": "369",
      "DOI": "10.1093/nar/16.1.369",
      "volume": "16",
      "author": "Green S.",
      "year": "1988",
      "unstructured": "Green S., Issemann I., Sheer E., Nucleic Acids Res. 16, 369 (1988).",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "e_1_3_1_28_2",
      "doi-asserted-by": "crossref",
      "unstructured": "W. Feng et al. Science 280 1747 (1998).",
      "DOI": "10.1126/science.280.5370.1747"
    },
    {
      "key": "e_1_3_1_29_2",
      "doi-asserted-by": "crossref",
      "first-page": "1139",
      "DOI": "10.1016/0092-8674(89)90051-2",
      "volume": "57",
      "author": "Umesono K.",
      "year": "1989",
      "unstructured": "Umesono K., Evans R. M., Cell 57, 1139 (1989).",
      "journal-title": "Cell"
    },
    {
      "key": "e_1_3_1_30_2",
      "unstructured": "We thank the following investigators for providing cDNA clones: H. R. Herschman (University of California Los Angeles) for PRMT1 R. M. Evans (Salk institute La Jolla CA) for ACTR M.-J. Tsai and B. W. O'Malley (Baylor College of Medicine Houston TX) for SRC-1a and A. O. Brinkmann (Erasmus University Rotterdam Netherlands) for androgen receptor. We thank S. Subramanian L. Yang and R. Widelitz for technical assistance and D. Johnson and R. Maxson (University of Southern California) for critical comments on the manuscript. Supported by USPHS grants DK43093 (M.R.S.) and NS17269 (D.W.A.) from the NIH and by a predoctoral traineeship from the University of California Breast Cancer Research Program (H.M.) and a predoctoral traineeship from grant AG00093 (S.S.K.) from the National Institutes of Health."
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