Crystal Structure of the Zα Domain of the Human Editing Enzyme ADAR1 Bound to Left-Handed Z-DNA
10.1126/science.284.5421.1841
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The editing enzyme double-stranded RNA adenosine deaminase includes a DNA binding domain, Zα, which is specific for left-handed Z-DNA. The 2.1 angstrom crystal structure of Zα complexed to DNA reveals that the substrate is in the left-handed Z conformation. The contacts between Zα and Z-DNA are made primarily with the “zigzag” sugar-phosphate backbone, which provides a basis for the specificity for the Z conformation. A single base contact is observed to guanine in the syn conformation, characteristic of Z-DNA. Intriguingly, the helix-turn-helix motif, frequently used to recognize B-DNA, is used by Zα to contact Z-DNA.

Bibliography

Schwartz, T., Rould, M. A., Lowenhaupt, K., Herbert, A., & Rich, A. (1999). Crystal Structure of the Zα Domain of the Human Editing Enzyme ADAR1 Bound to Left-Handed Z-DNA. Science, 284(5421), 1841–1845.

Authors 5
  1. Thomas Schwartz (first)
  2. Mark A. Rould (additional)
  3. Ky Lowenhaupt (additional)
  4. Alan Herbert (additional)
  5. Alexander Rich (additional)
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Dates
Type When
Created 23 years ago (July 27, 2002, 5:49 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 4:06 a.m.)
Indexed 3 weeks, 3 days ago (July 30, 2025, 11:21 a.m.)
Issued 26 years, 2 months ago (June 11, 1999)
Published 26 years, 2 months ago (June 11, 1999)
Published Print 26 years, 2 months ago (June 11, 1999)
Funders 0

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@article{Schwartz_1999, title={Crystal Structure of the Zα Domain of the Human Editing Enzyme ADAR1 Bound to Left-Handed Z-DNA}, volume={284}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.284.5421.1841}, DOI={10.1126/science.284.5421.1841}, number={5421}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Schwartz, Thomas and Rould, Mark A. and Lowenhaupt, Ky and Herbert, Alan and Rich, Alexander}, year={1999}, month=jun, pages={1841–1845} }