Interaction of RAFT1 with Gephyrin Required for Rapamycin-Sensitive Signaling
10.1126/science.284.5417.1161
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

RAFT1 (rapamycin and FKBP12 target 1; also called FRAP or mTOR) is a member of the ATM (ataxia telangiectasia mutated)–related family of proteins and functions as the in vivo mediator of the effects of the immunosuppressant rapamycin and as an important regulator of messenger RNA translation. In mammalian cells RAFT1 interacted with gephyrin, a widely expressed protein necessary for the clustering of glycine receptors at the cell membrane of neurons. RAFT1 mutants that could not associate with gephyrin failed to signal to downstream molecules, including the p70 ribosomal S6 kinase and the eIF-4E binding protein, 4E-BP1. The interaction with gephyrin ascribes a function to the large amino-terminal region of an ATM-related protein and reveals a role in signal transduction for the clustering protein gephyrin.

Bibliography

Sabatini, D. M., Barrow, R. K., Blackshaw, S., Burnett, P. E., Lai, M. M., Field, M. E., Bahr, B. A., Kirsch, J., Betz, H., & Snyder, S. H. (1999). Interaction of RAFT1 with Gephyrin Required for Rapamycin-Sensitive Signaling. Science, 284(5417), 1161–1164.

Authors 10
  1. David M. Sabatini (first)
  2. Roxanne K. Barrow (additional)
  3. Seth Blackshaw (additional)
  4. Patrick E. Burnett (additional)
  5. Michael M. Lai (additional)
  6. Michael E. Field (additional)
  7. Ben A. Bahr (additional)
  8. Joachim Kirsch (additional)
  9. Heinrich Betz (additional)
  10. Solomon H. Snyder (additional)
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  54. We thank T. Morimoto for help with HeLa cell fractionation A. Lanahan and P. Worley for the rat hippocampal library and L. Walensky N. Cohen and J. Lawler for fruitful discussions. Supported by U.S. Public Health Service grant DA-00266 and Research Scientist Award DA-00074 to S.H.S. and training grant GM-07309 to D.M.S.
Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:37 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 4:09 a.m.)
Indexed 3 weeks ago (Aug. 7, 2025, 4:31 p.m.)
Issued 26 years, 3 months ago (May 14, 1999)
Published 26 years, 3 months ago (May 14, 1999)
Published Print 26 years, 3 months ago (May 14, 1999)
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@article{Sabatini_1999, title={Interaction of RAFT1 with Gephyrin Required for Rapamycin-Sensitive Signaling}, volume={284}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.284.5417.1161}, DOI={10.1126/science.284.5417.1161}, number={5417}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sabatini, David M. and Barrow, Roxanne K. and Blackshaw, Seth and Burnett, Patrick E. and Lai, Michael M. and Field, Michael E. and Bahr, Ben A. and Kirsch, Joachim and Betz, Heinrich and Snyder, Solomon H.}, year={1999}, month=may, pages={1161–1164} }