Chaperonin Function: Folding by Forced Unfolding
10.1126/science.284.5415.822
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The ability of the GroEL chaperonin to unfold a protein trapped in a misfolded condition was detected and studied by hydrogen exchange. The GroEL-induced unfolding of its substrate protein is only partial, requires the complete chaperonin system, and is accomplished within the 13 seconds required for a single system turnover. The binding of nucleoside triphosphate provides the energy for a single unfolding event; multiple turnovers require adenosine triphosphate hydrolysis. The substrate protein is released on each turnover even if it has not yet refolded to the native state. These results suggest that GroEL helps partly folded but blocked proteins to fold by causing them first to partially unfold. The structure of GroEL seems well suited to generate the nonspecific mechanical stretching force required for forceful protein unfolding.

Bibliography

Shtilerman, M., Lorimer, G. H., & Walter Englander, S. (1999). Chaperonin Function: Folding by Forced Unfolding. Science, 284(5415), 822–825.

Authors 3 University of Pennsylvania
  1. Mark Shtilerman (first) University of Pennsylvania
  2. George H. Lorimer (additional)
  3. S. Walter Englander (additional) University of Pennsylvania
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Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:49 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 4:21 a.m.)
Indexed 1 month, 3 weeks ago (July 4, 2025, 7:47 a.m.)
Issued 26 years, 4 months ago (April 30, 1999)
Published 26 years, 4 months ago (April 30, 1999)
Published Print 26 years, 4 months ago (April 30, 1999)
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@article{Shtilerman_1999, title={Chaperonin Function: Folding by Forced Unfolding}, volume={284}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.284.5415.822}, DOI={10.1126/science.284.5415.822}, number={5415}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Shtilerman, Mark and Lorimer, George H. and Walter Englander, S.}, year={1999}, month=apr, pages={822–825} }