Inhibition of Myosin Light Chain Kinase by p21-Activated Kinase
10.1126/science.283.5410.2083
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

p21-activated kinases (PAKs) are implicated in the cytoskeletal changes induced by the Rho family of guanosine triphosphatases. Cytoskeletal dynamics are primarily modulated by interactions of actin and myosin II that are regulated by myosin light chain kinase (MLCK)–mediated phosphorylation of the regulatory myosin light chain (MLC). p21-activated kinase 1 (PAK1) phosphorylates MLCK, resulting in decreased MLCK activity. MLCK activity and MLC phosphorylation were decreased, and cell spreading was inhibited in baby hamster kidney–21 and HeLa cells expressing constitutively active PAK1. These data indicate that MLCK is a target for PAKs and that PAKs may regulate cytoskeletal dynamics by decreasing MLCK activity and MLC phosphorylation.

Bibliography

Sanders, L. C., Matsumura, F., Bokoch, G. M., & de Lanerolle, P. (1999). Inhibition of Myosin Light Chain Kinase by p21-Activated Kinase. Science, 283(5410), 2083–2085.

Authors 4
  1. Luraynne C. Sanders (first)
  2. Fumio Matsumura (additional)
  3. Gary M. Bokoch (additional)
  4. Primal de Lanerolle (additional)
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  41. Supported by a U.S. Army Research Breast Cancer Program award to L.C.S. and by grants from the U.S. Public Health Service to F.M. to G.M.B. and to P.d.L. This is publication 11936-IMM from The Scripps Research Institute.
Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:40 a.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 10:35 p.m.)
Indexed 1 month, 1 week ago (July 23, 2025, 9:03 a.m.)
Issued 26 years, 5 months ago (March 26, 1999)
Published 26 years, 5 months ago (March 26, 1999)
Published Print 26 years, 5 months ago (March 26, 1999)
Funders 0

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@article{Sanders_1999, title={Inhibition of Myosin Light Chain Kinase by p21-Activated Kinase}, volume={283}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.283.5410.2083}, DOI={10.1126/science.283.5410.2083}, number={5410}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Sanders, Luraynne C. and Matsumura, Fumio and Bokoch, Gary M. and de Lanerolle, Primal}, year={1999}, month=mar, pages={2083–2085} }