Role of DNA 5-Methylcytosine Transferase in Cell Transformation by fos
10.1126/science.283.5400.387
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The Fos and Jun oncoproteins form dimeric complexes that stimulate transcription of genes containing activator protein–1 regulatory elements. We found, by representational difference analysis, that expression of DNA 5-methylcytosine transferase ( dnmt1 ) in fos -transformed cells is three times the expression in normal fibroblasts and that fos -transformed cells contain about 20 percent more 5-methylcytosine than normal fibroblasts. Transfection of the gene encoding Dnmt1 induced morphological transformation, whereas inhibition of dnmt1 expression or activity resulted in reversion of fos transformation. Inhibition of histone deacetylase, which associates with methylated DNA, also caused reversion. These results suggest that fos may transform cells through alterations in DNA methylation and in histone deacetylation.

Bibliography

Bakin, A. V., & Curran, T. (1999). Role of DNA 5-Methylcytosine Transferase in Cell Transformation by fos. Science, 283(5400), 387–390.

Authors 2
  1. A. V. Bakin (first)
  2. T. Curran (additional)
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  56. We thank S. Baylin for the CMV dnmt1 expression vector; M. Szyf for antibodies to Dnmt1 and for plasmids containing the 5′-end region of the dnmt1 gene; T. Bestor for antibodies to mouse Dnmt1; and C. Sherr S. Baker and colleagues in the Curran lab for helpful discussions and critical comments on this manuscript. Supported in part by NIH Cancer Center Support CORE grant P30 CA21765 and by the American Lebanese Syrian Associated Charities (ALSAC).
Dates
Type When
Created 23 years ago (July 27, 2002, 5:40 a.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 10:34 p.m.)
Indexed 1 month, 3 weeks ago (July 2, 2025, 3:07 p.m.)
Issued 26 years, 7 months ago (Jan. 15, 1999)
Published 26 years, 7 months ago (Jan. 15, 1999)
Published Print 26 years, 7 months ago (Jan. 15, 1999)
Funders 0

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@article{Bakin_1999, title={Role of DNA 5-Methylcytosine Transferase in Cell Transformation by fos}, volume={283}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.283.5400.387}, DOI={10.1126/science.283.5400.387}, number={5400}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Bakin, A. V. and Curran, T.}, year={1999}, month=jan, pages={387–390} }