Germline Organization of the Murine T Cell Receptor β-Chain Genes
10.1126/science.2821625
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The complete germline organization of the β-chain genes of the murine T cell receptor was elucidated in order to obtain the structural basis for understanding the mechanisms of somatic DNA rearrangements. Twenty of the 22 known variable (V β ) genes are clustered within 250 kilobases of DNA 5′ to the constant region (C β ) genes. These V β genes share the same transcriptional orientation as the diversity (D β ), joining (J β ), and C β genes, which implies that chromosomal deletion is the mechanism for most V β to D β -J β rearrangements. Within this V β cluster, the distance between the most proximal V β gene and the D β -J β -C β cluster is 320 kilobases, as determined by field-inversion gel electrophoresis. The large distance between V β and D β , relative to that between D β and J β , may have significant implications for the ordered rearrangement of the T cell receptor β-chain genes.

Bibliography

Chou, H. S., Nelson, C. A., Godambe, S. A., Chaplin, D. D., & Loh, D. Y. (1987). Germline Organization of the Murine T Cell Receptor β-Chain Genes. Science, 238(4826), 545–548.

Authors 5
  1. Hubert S. Chou (first)
  2. Christopher A. Nelson (additional)
  3. Sandip A. Godambe (additional)
  4. David D. Chaplin (additional)
  5. Dennis Y. Loh (additional)
References 40 Referenced 38
  1. ALESSANDRINI, A, CONTINUING REARRANGEMENT OF IMMUNOGLOBULIN AND T-CELL RECEPTOR GENES IN A HA-RAS-TRANSFORMED LYMPHOID PROGENITOR-CELL LINE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 84: 1799 (1987). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1987)
  2. ALLISON, J.P., TUMOR-SPECIFIC ANTIGEN OF MURINE T-LYMPHOMA DEFINED WITH MONOCLONAL-ANTIBODY, JOURNAL OF IMMUNOLOGY 129: 2293 (1982). (10.4049/jimmunol.129.5.2293) / JOURNAL OF IMMUNOLOGY (1982)
  3. BARTH, R.K., THE MURINE T-CELL RECEPTOR USES A LIMITED REPERTOIRE OF EXPRESSED V-BETA GENE SEGMENTS, NATURE 316: 517 (1985). (10.1038/316517a0) / NATURE (1985)
  4. BEHLKE, M.A., EXPRESSION OF A MURINE POLYCLONAL T-CELL RECEPTOR MARKER CORRELATES WITH THE USE OF SPECIFIC MEMBERS OF THE V-BETA-8 GENE SEGMENT SUBFAMILY, JOURNAL OF EXPERIMENTAL MEDICINE 165: 257 (1987). (10.1084/jem.165.1.257) / JOURNAL OF EXPERIMENTAL MEDICINE (1987)
  5. BEHLKE, M.A., MURINE T-CELL RECEPTOR MUTANTS WITH DELETIONS OF BETA-CHAIN VARIABLE REGION GENES, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 767 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
  6. 10.1126/science.3875151
  7. BORN, W, REARRANGEMENT OF T-CELL RECEPTOR BETA-CHAIN GENES DURING T-CELL DEVELOPMENT, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 82: 2925 (1985). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1985)
  8. 10.1126/science.3952500
  9. CHAPLIN, D.D., MOLECULAR MAP OF THE MURINE S-REGION, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES 80: 6947 (1983). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES (1983)
  10. 10.1038/309322a0
  11. CHOU, H.S., T-CELL RECEPTOR GENES IN AN ALLOREACTIVE CTL CLONE - IMPLICATIONS FOR REARRANGEMENT AND GERMLINE DIVERSITY OF VARIABLE GENE SEGMENTS, EMBO JOURNAL 5: 2149 (1986). (10.1002/j.1460-2075.1986.tb04478.x) / EMBO JOURNAL (1986)
  12. 10.1073/pnas.84.7.1992
  13. 10.1016/0003-2697(83)90418-9
  14. FUJIMOTO, S, ISOLATION OF AN EXCISION PRODUCT OF T-CELL RECEPTOR ALPHA-CHAIN GENE REARRANGEMENTS, NATURE 327: 242 (1987). (10.1038/327242a0) / NATURE (1987)
  15. GARMAN, R.D., T-CELL RECEPTOR VARIABLE REGION GENE USAGE IN T-CELL POPULATIONS, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 3987 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
  16. 10.1038/310387a0
  17. 10.1016/0092-8674(85)90345-9
  18. HASKINS, K, THE MAJOR HISTOCOMPATIBILITY COMPLEX-RESTRICTED ANTIGEN RECEPTOR ON T-CELLS .1. ISOLATION WITH A MONOCLONAL-ANTIBODY, JOURNAL OF EXPERIMENTAL MEDICINE 157: 1149 (1983). (10.1084/jem.157.4.1149) / JOURNAL OF EXPERIMENTAL MEDICINE (1983)
  19. IWAMOTO, A, T-CELL-SPECIFIC GAMMA-GENES IN C57BL 10 MICE - SEQUENCE AND EXPRESSION OF NEW CONSTANT AND VARIABLE REGION GENES, JOURNAL OF EXPERIMENTAL MEDICINE 163: 1203 (1986). (10.1084/jem.163.5.1203) / JOURNAL OF EXPERIMENTAL MEDICINE (1986)
  20. KAPPLER, J.W., A T-CELL RECEPTOR VBETA-SEGMENT THAT IMPARTS REACTIVITY TO A CLASS-II MAJOR HISTOCOMPATIBILITY COMPLEX PRODUCT, CELL 49: 263 (1987). (10.1016/0092-8674(87)90567-8) / CELL (1987)
  21. 10.1038/310421a0
  22. 10.1146/annurev.iy.04.040186.002525
  23. KUROSAWA, Y, IDENTIFICATION OF D-SEGMENTS OF IMMUNOGLOBULIN HEAVY-CHAIN GENES AND THEIR REARRANGEMENT IN LYMPHOCYTES-T, NATURE 290: 565 (1981). (10.1038/290565a0) / NATURE (1981)
  24. 10.1016/0092-8674(84)90444-6
  25. 10.1038/319028a0
  26. MEUER, S. C., JOURNAL OF EXPERIMENTAL MEDICINE 157: 705 (1983). (10.1084/jem.157.2.705) / JOURNAL OF EXPERIMENTAL MEDICINE (1983)
  27. OKAZAKI, K, T-CELL RECEPTOR BETA-GENE SEQUENCES IN THE CIRCULAR DNA OF THYMOCYTE NUCLEI - DIRECT EVIDENCE FOR INTRAMOLECULAR DNA DELETION IN V-D-J JOINING, CELL 49: 477 (1987). (10.1016/0092-8674(87)90450-8) / CELL (1987)
  28. PATTEN, P, STRUCTURE, EXPRESSION AND DIVERGENCE OF T-CELL RECEPTOR BETA-CHAIN VARIABLE REGIONS, NATURE 312: 40 (1984). (10.1038/312040a0) / NATURE (1984)
  29. 10.1126/science.3975629
  30. RETH, M.G., NOVEL IMMUNOGLOBULIN HEAVY-CHAINS ARE PRODUCED FROM DJH GENE SEGMENT REARRANGEMENTS IN LYMPHOID-CELLS, NATURE 312: 418 (1984). (10.1038/312418a0) / NATURE (1984)
  31. SIM, G.K., V-BETA GENE POLYMORPHISM AND A MAJOR POLYCLONAL T-CELL RECEPTOR IDIOTYPE, CELL 42: 89 (1985). (10.1016/S0092-8674(85)80104-5) / CELL (1985)
  32. SINGER, P.A., CLONAL DIVERSITY AND T-CELL RECEPTOR BETA-CHAIN VARIABLE GENE-EXPRESSION IN ENLARGED LYMPH-NODES OF MRL-LPR-LPR LUPUS MICE, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 83: 7018 (1986). / PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (1986)
  33. 10.1016/0092-8674(84)90369-6
  34. SIU, G, NATURE 311: 387 (1984). (10.1038/311387a0) / NATURE (1984)
  35. Smith, C. L., Methods in Enzymology 151: 461 (1987). (10.1016/S0076-6879(87)51038-2) / Methods in Enzymology (1987)
  36. 10.1038/302575a0
  37. WU, G.E., VH GENE FAMILY UTILIZATION IN COLONIES DERIVED FROM B AND PRE-B CELLS DETECTED BY THE RNA COLONY BLOT ASSAY, EMBO JOURNAL 5: 3475 (1986). (10.1002/j.1460-2075.1986.tb04672.x) / EMBO JOURNAL (1986)
  38. YANCOPOULOS, G.D., REGULATION OF THE ASSEMBLY AND EXPRESSION OF VARIABLE-REGION GENES, ANNUAL REVIEW OF IMMUNOLOGY 4: 339 (1986). (10.1146/annurev.iy.04.040186.002011) / ANNUAL REVIEW OF IMMUNOLOGY (1986)
  39. YANCOPOULOS, G.D., INTRODUCED T-CELL RECEPTOR VARIABLE REGION GENE SEGMENTS RECOMBINE IN PRE-B-CELLS - EVIDENCE THAT B-CELL AND T-CELL USE A COMMON RECOMBINASE, CELL 44: 251 (1986). (10.1016/0092-8674(86)90759-2) / CELL (1986)
  40. YANCOPOULOS, G.D., PREFERENTIAL UTILIZATION OF THE MOST JH-PROXIMAL VH GENE SEGMENTS IN PRE-B-CELL LINES, NATURE 311: 727 (1984). (10.1038/311727a0) / NATURE (1984)
Dates
Type When
Created 18 years, 10 months ago (Oct. 5, 2006, 4:28 p.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 10:59 p.m.)
Indexed 1 year, 7 months ago (Jan. 13, 2024, 11:02 a.m.)
Issued 37 years, 10 months ago (Oct. 23, 1987)
Published 37 years, 10 months ago (Oct. 23, 1987)
Published Print 37 years, 10 months ago (Oct. 23, 1987)
Funders 0

None

@article{Chou_1987, title={Germline Organization of the Murine T Cell Receptor β-Chain Genes}, volume={238}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.2821625}, DOI={10.1126/science.2821625}, number={4826}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Chou, Hubert S. and Nelson, Christopher A. and Godambe, Sandip A. and Chaplin, David D. and Loh, Dennis Y.}, year={1987}, month=oct, pages={545–548} }