Localization of Bacterial DNA Polymerase: Evidence for a Factory Model of Replication
10.1126/science.282.5393.1516
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Two general models have been proposed for DNA replication. In one model, DNA polymerase moves along the DNA (like a train on a track); in the other model, the polymerase is stationary (like a factory), and DNA is pulled through. To distinguish between these models, we visualized DNA polymerase of the bacterium Bacillus subtilis in living cells by the creation of a fusion protein containing the catalytic subunit (PolC) and green fluorescent protein (GFP). PolC-GFP was localized at discrete intracellular positions, predominantly at or near midcell, rather than being distributed randomly. These results suggest that the polymerase is anchored in place and thus support the model in which the DNA template moves through the polymerase.

Bibliography

Lemon, K. P., & Grossman, A. D. (1998). Localization of Bacterial DNA Polymerase: Evidence for a Factory Model of Replication. Science, 282(5393), 1516–1519.

Authors 2
  1. Katherine P. Lemon (first)
  2. Alan D. Grossman (additional)
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Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:44 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 12:33 a.m.)
Indexed 1 week, 2 days ago (Aug. 20, 2025, 8:56 a.m.)
Issued 26 years, 9 months ago (Nov. 20, 1998)
Published 26 years, 9 months ago (Nov. 20, 1998)
Published Print 26 years, 9 months ago (Nov. 20, 1998)
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@article{Lemon_1998, title={Localization of Bacterial DNA Polymerase: Evidence for a Factory Model of Replication}, volume={282}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.282.5393.1516}, DOI={10.1126/science.282.5393.1516}, number={5393}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Lemon, Katherine P. and Grossman, Alan D.}, year={1998}, month=nov, pages={1516–1519} }