DOI: 10.1126/science.282.5391.1136. Transformation of Tooth Type Induced by Inhibition of BMP Signaling

Transformation of Tooth Type Induced by Inhibition of BMP Signaling

10.1126/science.282.5391.1136

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Mammalian dentitions are highly patterned, with different types of teeth positioned in different regions of the jaws. BMP4 is an early oral epithelial protein signal that directs odontogenic gene expression in mesenchyme cells of the developing mandibular arch. BMP4 was shown to inhibit expression of the homeobox gene Barx-1 and to restrict expression to the proximal, presumptive molar mesenchyme of mouse embryos at embryonic day 10. The inhibition of BMP signaling early in mandible development by the action of exogenous Noggin protein resulted in ectopic Barx-1 expression in the distal, presumptive incisor mesenchyme and a transformation of tooth identity from incisor to molar.

Authors 3

Abigail S. Tucker (first)
Karen L. Matthews (additional)
Paul T. Sharpe (additional)

References 20 Referenced 353

Vainio S., Karavanova I., Jowett A., Thesleff I., Cell 75, 45 (1993). (10.1016/S0092-8674(05)80083-2) / Cell by Vainio S. (1993)
Tissier-Seta J. P., et al., Mech. Dev. 51, 3 (1995). (10.1016/0925-4773(94)00343-L) / Mech. Dev. by Tissier-Seta J. P. (1995)
Tucker A. S., Al Khamis A., Sharpe P. T., Dev. Dyn. 212, 533 (1998). (10.1002/(SICI)1097-0177(199808)212:4<533::AID-AJA6>3.0.CO;2-I) / Dev. Dyn. by Tucker A. S. (1998)
Grigoriou M., Tucker A. S., Sharpe P. T., Pachnis V., Development 125, 2063 (1998). (10.1242/dev.125.11.2063) / Development by Grigoriou M. (1998)
Mandibular arch explants were dissected and cultured as described (3). Beads were also prepared as described (3 4) with Fgf8 and BSA (white) and BMP4 and Noggin (blue) beads at a concentration of 100 μg/mg. Whole-mount digoxigenin (DIG) and sectioned 35 S in situ hybridization were carried out as described (3 4).
A. S. Tucker K. L. Matthews P. T. Sharpe data not shown.
Houzelstein D., Cohen A., Buckingham M. E., Robert B., Mech. Dev. 65, 123 (1997). (10.1016/S0925-4773(97)00065-8) / Mech. Dev. by Houzelstein D. (1997)
10.1016/S0092-8674(00)80133-6
Raju K., et al., Mech. Dev. 44, 51 (1993); (10.1016/0925-4773(93)90016-Q) / Mech. Dev. by Raju K. (1993)
. Tlx-1 +/– embryos were produced by the insertion of a nlacZ reporter gene and were stained with β-galactosidase as described [
Dear T. N., et al., Development 121, 2909 (1995)]. (10.1242/dev.121.9.2909) / Development by Dear T. N. (1995)
E10 and E11 mandibular arch explants were cultured with beads that were placed in the distal (presumptive incisor) mesenchyme for 48 hours in vitro. These were then dissected into incisor and molar regions; the incisor regions were dissected as pairs. Single incisor and molar regions from each mandibular arch were transferred into opposite ends of a kidney under the kidney capsule in adult male mice. These regions were then left to develop for 14 days in vivo. The resulting teeth were photographed placed in Bouin's fixative for 3 days dehydrated and embedded. Sections were stained with alcian blue and chlorontine fast red.
Ferguson C. A., et al., Genes Dev. 12, 2636 (1998). (10.1101/gad.12.16.2636) / Genes Dev. by Ferguson C. A. (1998)
Kantaputra P. N., Gorlin R. J., Clin. Dysmorphol. 1, 128 (1992). (10.1097/00019605-199207000-00002) / Clin. Dysmorphol. by Kantaputra P. N. (1992)
Thomas B. L., Sharpe P. T., Euro. J. Oral Sci. 106, 48 (1998). (10.1111/j.1600-0722.1998.tb02153.x) / Euro. J. Oral Sci. by Thomas B. L. (1998)
Thomas B. L., et al., Development 124, 4811 (1997). (10.1242/dev.124.23.4811) / Development by Thomas B. L. (1997)
10.1016/0012-1606(83)90318-4
10.1242/dev.122.10.3229
Neubüser A., Peters H., Balling R., Martin G. R., Cell 90, 247 (1997). (10.1016/S0092-8674(00)80333-5) / Cell by Neubüser A. (1997)
We thank R. Harland and J. de Jesús for the recombinant Noggin protein; the Genetics Institute for BMP4; T. Rabbitts for mice carrying Tlx-1 ; B. Robert for Msx-1 mutants; C. Ferguson B. Thomas N. Young and C. Healy for comments on the manuscript; and the UK Medical Research Council and the Human Frontier Science Program for grant support.

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:50 a.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 12:13 a.m.)
Indexed	3 weeks, 5 days ago (Aug. 7, 2025, 5:18 p.m.)
Issued	26 years, 9 months ago (Nov. 6, 1998)
Published	26 years, 9 months ago (Nov. 6, 1998)
Published Print	26 years, 9 months ago (Nov. 6, 1998)

Funders 0

None

BibTeX

@article{Tucker_1998, title={Transformation of Tooth Type Induced by Inhibition of BMP Signaling}, volume={282}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.282.5391.1136}, DOI={10.1126/science.282.5391.1136}, number={5391}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Tucker, Abigail S. and Matthews, Karen L. and Sharpe, Paul T.}, year={1998}, month=nov, pages={1136–1138} }

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