DOI: 10.1126/science.282.5388.430. RNAi in <i>C. elegans</i> : Soaking in the Genome Sequence

RNAi in C. elegans : Soaking in the Genome Sequence

10.1126/science.282.5388.430

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Bibliography

Tabara, H., Grishok, A., & Mello, C. C. (1998). RNAi in C. elegans â¯: Soaking in the Genome Sequence. Science, 282(5388), 430â431.

Authors 3

Hiroaki Tabara (first)
Alla Grishok (additional)
Craig C. Mello (additional)

References 17 Referenced 483

10.1016/0092-8674(95)90082-9
10.1038/35888
10.1016/S0092-8674(00)80531-0
Timmons L., Fire A., Naturein press. / Nature by Timmons L.
Grishok A. Mello C. C. unpublished results. We do not know whether this long-term transmission which is always somewhat less penetrant than the interference observed in the first two generations involves the same or a secondary interference mechanism.
10.1242/dev.125.13.2469
Bosher J. Labouesse M. personal communication.
Montgomery M. Fire A. personal communication.
Powell-Coffman J. A., Knight J., Wood W. B., Dev. Biol.178, 472 (1996). (10.1006/dbio.1996.0232) / Dev. Biol. by Powell-Coffman J. A. (1996)
Tabara H. Mello C. C. unpublished results.
Wagner R. W., Sun L., Nature391, 744 (1998). (10.1038/35750) / Nature by Wagner R. W. (1998)
Mello C. C. unpublished observations.
In one case we found that two genes that were 80% identical showed clear evidence of cross-interference. Specific interference was achieved by diluting the injected RNA from 1 mg/ml to 100 g/ml. (12).
L4-stage hermaphrodites were washed in 0.2 M sucrose and 0.1 phosphate-buffered saline and transferred into 10 l of the same buffer in a siliconized tube. In another siliconized tube 4 l of dsRNA (3.8 mg/ml) and 1 l liposome (Lipofectin Gibco-BRL) were vigorously mixed. Fifteen worms were added to the RNA-liposome mixture resulting in a total volume of 15 l and a final RNA concentration of 1 mg/ml. After 24 hours the worms were transferred to an agar plate with E. coli and cultured until mid-adulthood. Similar levels of interference were obtained by feeding worms E. coli expressing both strands of pos-1 RNA (10).
Tabara H. Mello C. C. unpublished observations.
Montgomery M. K., Fire A., Trends Genet14, 255 (1998). (10.1016/S0168-9525(98)01510-8) / Trends Genet by Montgomery M. K. (1998)
Bosher J. Labouesse M. Timmons L. Fire A. We thankfor communicating unpublished results. Work in this laboratory is supported by NIH grant R01 HD33769-01 a pilot and feasibility project grant DK32520-15 and a Pew scholarship to C.C.M.

Dates

Type	When
Created	23 years, 1 month ago (July 27, 2002, 5:35 a.m.)
Deposited	1 year, 7 months ago (Jan. 13, 2024, 12:11 a.m.)
Indexed	1 day, 23 hours ago (Aug. 31, 2025, 6:36 a.m.)
Issued	26 years, 10 months ago (Oct. 16, 1998)
Published	26 years, 10 months ago (Oct. 16, 1998)
Published Print	26 years, 10 months ago (Oct. 16, 1998)

Funders 0

None

BibTeX

@article{Tabara_1998, title={RNAi in C. elegans  : Soaking in the Genome Sequence}, volume={282}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.282.5388.430}, DOI={10.1126/science.282.5388.430}, number={5388}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Tabara, Hiroaki and Grishok, Alla and Mello, Craig C.}, year={1998}, month=oct, pages={430–431} }

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