DOI: 10.1126/science.281.5385.2016. Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection

Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection

10.1126/science.281.5385.2016

Crossref journal-article

American Association for the Advancement of Science (AAAS)

Science (221)

Abstract

Highly luminescent semiconductor quantum dots (zinc sulfide–capped cadmium selenide) have been covalently coupled to biomolecules for use in ultrasensitive biological detection. In comparison with organic dyes such as rhodamine, this class of luminescent labels is 20 times as bright, 100 times as stable against photobleaching, and one-third as wide in spectral linewidth. These nanometer-sized conjugates are water-soluble and biocompatible. Quantum dots that were labeled with the protein transferrin underwent receptor-mediated endocytosis in cultured HeLa cells, and those dots that were labeled with immunomolecules recognized specific antibodies or antigens.

Bibliography

Chan, W. C. W., & Nie, S. (1998). Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection. Science, 281(5385), 2016â2018.

Authors 2

Warren C. W. Chan (first)
Shuming Nie (additional)

References 36 Referenced 6,201

For recent books see L. J. Kricka Ed. Nonisotopic Probing Blotting and Sequencing (Academic Press New York 1995); P. G. Issac Ed. Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Humana Totowa NJ 1994); E. P. Diamandis and T. K. Christopoulos Eds. Immunoassay (Academic Press New York 1996).
10.1126/science.271.5251.933
; J. Phys. Chem. 100 13226 (1996). (10.1021/jp9535506)
10.1007/BF00331535
10.1126/science.262.5137.1242
Henglein A., Chem. Rev. 89, 1861 (1989); (10.1021/cr00098a010) / Chem. Rev. by Henglein A. (1989)
Weller H., Angew. Chem. Int. Ed. Engl. 32, 41 (1993). (10.1002/anie.199300411) / Angew. Chem. Int. Ed. Engl. by Weller H. (1993)
10.1021/ja00072a025
Bowen Katari J. E., Colvin V. L., Alivisatos A. P., J. Phys. Chem. 98, 4109 (1994). (10.1021/j100066a034) / J. Phys. Chem. by Bowen Katari J. E. (1994)
Hines M. A., Guyot-Sionnest P., ibid. 100, 468 (1996). / ibid. by Hines M. A. (1996)
10.1126/science.270.5240.1335
Andres R. P., et al., ibid. 273, 1690 (1996). / ibid. by Andres R. P. (1996)
Heath J. R., et al., J. Phys. Chem. 100, 3144 (1996); (10.1021/jp951903v) / J. Phys. Chem. by Heath J. R. (1996)
Collier C. P., Saykally R. J., Shiang J. J., Henrichs S. E., Heath J. R., Science 277, 1978 (1997). (10.1126/science.277.5334.1978) / Science by Collier C. P. (1997)
10.1038/382607a0
A. P. Alivisatos et al. ibid. p. 609.
Colvin V. L., Schlamp M. C., Alivisatos A. P., ibid. 370, 354 (1994). / ibid. by Colvin V. L. (1994)
10.1063/1.113227
Dabbousi B. O., et al., J. Phys. Chem. B 101, 9463 (1997). (10.1021/jp971091y) / J. Phys. Chem. B by Dabbousi B. O. (1997)
G. T. Hermanson Bioconjugate Techniques (Academic Press New York 1996). (10.1016/B978-012342335-1/50012-9)
W. C. W. Chan and S. Nie unpublished data.
10.1038/41048
Lu H. P., Xie X. X., ibid. 385, 143 (1997). / ibid. by Lu H. P. (1997)
Vanden D. A., et al., Science 277, 1074 (1997). (10.1126/science.277.5329.1074) / Science by Vanden D. A. (1997)
Nirmal M., et al., Nature 383, 802 (1996); (10.1038/383802a0) / Nature by Nirmal M. (1996)
Empedocles S. A., Norris D. J., Bawendi M. G., Phys. Rev. Lett. 77, 3873 (1996). (10.1103/PhysRevLett.77.3873) / Phys. Rev. Lett. by Empedocles S. A. (1996)
Blanton S. A., Hines M. A., Guyot-Sionnest P., Appl. Phys. Lett. 69, 3905 (1996). (10.1063/1.117565) / Appl. Phys. Lett. by Blanton S. A. (1996)
The 40-nm fluorescent latex spheres (λ ex = 530 nm; λ em = 560 nm) (λ ex excitation wavelength maximum; λ em emission wavelength maximum) were purchased from Molecular Probes (Eugene OR). Each sphere is equivalent in intensity to ∼350 dye molecules [R. P. Haugland Handbook of Fluorescent Probes and Research Chemicals (Molecular Probes Eugene OR ed. 6 1995)]. The integrated emission intensities for single QDs and single latex spheres were measured and compared under identical experimental conditions. The single-dot photoluminescence was about one-seventeenth that of a single latex sphere and thus was estimated to be equal to the total emission intensity of 20 R6G molecules.
Peck K., Stryer L., Glazer A. N., Mathies R. A., Proc. Natl. Acad. Sci. U.S.A. 86, 4087 (1989). (10.1073/pnas.86.11.4087) / Proc. Natl. Acad. Sci. U.S.A. by Peck K. (1989)
Edwards D. A., Gooch K. J., Zhang I., McKinley G. H., Langer R., ibid. 93, 1786 (1996); / ibid. by Edwards D. A. (1996)
Smythe E., Warren G., Eur. J. Biochem. 202, 689 (1991). (10.1111/j.1432-1033.1991.tb16424.x) / Eur. J. Biochem. by Smythe E. (1991)
Quantitative measurements showed that the intensity variations among the discrete luminescent signals were within a factor of 3. Thus the observed signals arose primarily from single QDs and perhaps a small population of double- and triple-dot aggregates. Protein BSA should adsorb nonspecifically on the QDs but it did not cause particle aggregation.
Bangs L. B., Pure Appl. Chem. 68, 1873 (1996). (10.1351/pac199668101873) / Pure Appl. Chem. by Bangs L. B. (1996)
Speicher M. R., Ballard S. G., Ward D. C., Nature Genet. 12, 368 (1996). (10.1038/ng0496-368) / Nature Genet. by Speicher M. R. (1996)
10.1021/ja9805425
We are grateful to M. A. Hines and P. Guyot-Sionnest for their help in QD synthesis and for providing the original ZnS-capped CdSe sample. We also thank A. K. Reuter for bulk optical measurement R. Turner for TEM and S. R. Emory for valuable discussions. This work was supported in part by NSF grant CHE-9610254 and by the Petroleum Research Fund grant 32231-AC5. S.N. acknowledges the Whitaker Foundation for a Biomedical Engineering Award and the Beckman Foundation for a Beckman Young Investigator Award.

Dates

Type	When
Created	23 years ago (July 27, 2002, 5:50 a.m.)
Deposited	1 year, 7 months ago (Jan. 12, 2024, 9:45 p.m.)
Indexed	3 days, 14 hours ago (Aug. 19, 2025, 6:01 a.m.)
Issued	26 years, 10 months ago (Sept. 25, 1998)
Published	26 years, 10 months ago (Sept. 25, 1998)
Published Print	26 years, 10 months ago (Sept. 25, 1998)

Funders 0

None

BibTeX

@article{Chan_1998, title={Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection}, volume={281}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.281.5385.2016}, DOI={10.1126/science.281.5385.2016}, number={5385}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Chan, Warren C. W. and Nie, Shuming}, year={1998}, month=sep, pages={2016–2018} }

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