Thymocyte Development in the Absence of Pre-T Cell Receptor Extracellular Immunoglobulin Domains
10.1126/science.280.5365.905
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Immature thymocytes express a pre–T cell receptor (pre-TCR) composed of the TCRβ chain paired with pre-Tα. Signals from this receptor are essential for passage of thymocytes through a key developmental checkpoint in the thymus. These signals were efficiently delivered in vivo by a truncated form of the murine pre-TCR that lacked all of its extracellular immunoglobulin domains. De novo expression of the truncated pre-TCR or an intact αβTCR was sufficient to activate characteristic TCR signaling pathways in a T cell line. These findings support the view that recognition of an extracellular ligand is not required for pre-TCR function.

Bibliography

Irving, B. A., Alt, F. W., & Killeen, N. (1998). Thymocyte Development in the Absence of Pre-T Cell Receptor Extracellular Immunoglobulin Domains. Science, 280(5365), 905–908.

Authors 3
  1. Bryan A. Irving (first)
  2. Frederick W. Alt (additional)
  3. Nigel Killeen (additional)
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  48. We thank D. H. Raulet J. G. Cyster A. Weiss and members of the Killeen laboratory for critical review of the manuscript; A. Weiss for provision of plasmids the TCRβ-deficient Jurkat cell line and discussions; P. Mombaerts for θ8c thymoma; W. Swat for the Rag-1 −/− ES cells; and J. D. Scarborough for help with the production of transgenic mice. Supported by the Irvington Institute (B.A.I.) the Lucille P. Markey Foundation through the University of California San Francisco Program in Biological Sciences (N.K.) and the Howard Hughes Medical Institute (F.W.A.).
Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:42 a.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 10:35 p.m.)
Indexed 2 months, 2 weeks ago (June 11, 2025, 8:25 a.m.)
Issued 27 years, 3 months ago (May 8, 1998)
Published 27 years, 3 months ago (May 8, 1998)
Published Print 27 years, 3 months ago (May 8, 1998)
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@article{Irving_1998, title={Thymocyte Development in the Absence of Pre-T Cell Receptor Extracellular Immunoglobulin Domains}, volume={280}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.280.5365.905}, DOI={10.1126/science.280.5365.905}, number={5365}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Irving, Bryan A. and Alt, Frederick W. and Killeen, Nigel}, year={1998}, month=may, pages={905–908} }