Supramolecular Structure of the Salmonella typhimurium Type III Protein Secretion System
10.1126/science.280.5363.602
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

The type III secretion system of Salmonella typhimurium directs the translocation of proteins into host cells. Evolutionarily related to the flagellar assembly machinery, this system is also present in other pathogenic bacteria, but its organization is unknown. Electron microscopy revealed supramolecular structures spanning the inner and outer membranes of flagellated and nonflagellated strains; such structures were not detected in strains carrying null mutations in components of the type III apparatus. Isolated structures were found to contain at least three proteins of this secretion system. Thus, the type III apparatus of S. typhimurium , and presumably other bacteria, exists as a supramolecular structure in the bacterial envelope.

Bibliography

Kubori, T., Matsushima, Y., Nakamura, D., Uralil, J., Lara-Tejero, M., Sukhan, A., Galán, J. E., & Aizawa, S.-I. (1998). Supramolecular Structure of the Salmonella typhimurium Type III Protein Secretion System. Science, 280(5363), 602–605.

Authors 8
  1. Tomoko Kubori (first)
  2. Yukiyasu Matsushima (additional)
  3. Dai Nakamura (additional)
  4. Jaimol Uralil (additional)
  5. Maria Lara-Tejero (additional)
  6. Anand Sukhan (additional)
  7. Jorge E. Galán (additional)
  8. Shin-Ichi Aizawa (additional)
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  21. We thank S. Yamaguchi for Salmonella flagellar mutant strains; M. Iwakura for amino acid sequence analysis; S. Makishima T. Kubo and N. Kobayashi for assistance in the needle preparation; and J. Bliska R. Donis and members of the Galán laboratory for critical reading of this manuscript. Supported by a Grant-in-Aid for Scientific Research from the Ministry of Education Science Sports and Culture (S.-I.A.) the American Heart Association and Public Health Service Grants from the NIH (J.E.G.).
Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 1:42 a.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 7:21 p.m.)
Indexed 4 days, 12 hours ago (Aug. 29, 2025, 6:37 a.m.)
Issued 27 years, 4 months ago (April 24, 1998)
Published 27 years, 4 months ago (April 24, 1998)
Published Print 27 years, 4 months ago (April 24, 1998)
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@article{Kubori_1998, title={Supramolecular Structure of the Salmonella typhimurium Type III Protein Secretion System}, volume={280}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.280.5363.602}, DOI={10.1126/science.280.5363.602}, number={5363}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Kubori, Tomoko and Matsushima, Yukiyasu and Nakamura, Dai and Uralil, Jaimol and Lara-Tejero, Maria and Sukhan, Anand and Galán, Jorge E. and Aizawa, Shin-Ichi}, year={1998}, month=apr, pages={602–605} }