Ribosome-Catalyzed Peptide-Bond Formation with an A-Site Substrate Covalently Linked to 23 S Ribosomal RNA
10.1126/science.280.5361.286
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

In the ribosome, the aminoacyl–transfer RNA (tRNA) analog 4-thio-dT-p-C-p-puromycin crosslinks photochemically with G2553 of 23 S ribosomal RNA (rRNA). This covalently linked substrate reacts with a peptidyl-tRNA analog to form a peptide bond in a peptidyl transferase–catalyzed reaction. This result places the conserved 2555 loop of 23 S rRNA at the peptidyl transferase A site and suggests that peptide bond formation can occur uncoupled from movement of the A-site tRNA. Crosslink formation depends on occupancy of the P site by a tRNA carrying an intact CCA acceptor end, indicating that peptidyl-tRNA, directly or indirectly, helps to create the peptidyl transferase A site.

Bibliography

Green, R., Switzer, C., & Noller, H. F. (1998). Ribosome-Catalyzed Peptide-Bond Formation with an A-Site Substrate Covalently Linked to 23 S Ribosomal RNA. Science, 280(5361), 286–289.

Authors 3
  1. Rachel Green (first)
  2. Christopher Switzer (additional)
  3. Harry F. Noller (additional)
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  31. We thank B. Cormack G. Culver L. Holmberg K. Fredrick K. Lieberman and E. Strauss for helpful comments on the manuscript and S. Joseph and C. Merryman for helpful discussions. Supported by grants from the National Institutes of Health the National Science Foundation the Lucille P. Markey Charitable Trust to the Center for Molecular Biology of RNA and a Burroughs-Wellcome Career Award to R.G.
Dates
Type When
Created 23 years ago (July 27, 2002, 5:42 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 12:13 a.m.)
Indexed 1 year, 2 months ago (June 3, 2024, 1:55 a.m.)
Issued 27 years, 4 months ago (April 10, 1998)
Published 27 years, 4 months ago (April 10, 1998)
Published Print 27 years, 4 months ago (April 10, 1998)
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@article{Green_1998, title={Ribosome-Catalyzed Peptide-Bond Formation with an A-Site Substrate Covalently Linked to 23 S Ribosomal RNA}, volume={280}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.280.5361.286}, DOI={10.1126/science.280.5361.286}, number={5361}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Green, Rachel and Switzer, Christopher and Noller, Harry F.}, year={1998}, month=apr, pages={286–289} }