Expansion and Length-Dependent Fragility of CTG Repeats in Yeast
10.1126/science.279.5352.853
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Expansion of DNA trinucleotide repeats (TNRs) is the causative mutation in a growing number of human genetic diseases. Large expansions of a CTG tract were obtained and shown by genetic and physical assays to be length-dependent sites of chromosome breakage in Saccharomyces cerevisiae . Deletion of RAD27 , which encodes a nuclease involved in Okazaki fragment processing, caused length-dependent destabilization of CTG tracts and a substantial increase in expansion frequency. The genetic assay described here can be used to evaluate other factors that induce TNR expansion or chromosome fragility in humans.

Bibliography

Freudenreich, C. H., Kantrow, S. M., & Zakian, V. A. (1998). Expansion and Length-Dependent Fragility of CTG Repeats in Yeast. Science, 279(5352), 853–856.

Authors 3
  1. Catherine H. Freudenreich (first)
  2. Sara M. Kantrow (additional)
  3. Virginia A. Zakian (additional)
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Dates
Type When
Created 23 years ago (July 27, 2002, 5:37 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 1 a.m.)
Indexed 2 weeks, 5 days ago (Aug. 5, 2025, 8:41 a.m.)
Issued 27 years, 6 months ago (Feb. 6, 1998)
Published 27 years, 6 months ago (Feb. 6, 1998)
Published Print 27 years, 6 months ago (Feb. 6, 1998)
Funders 0

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@article{Freudenreich_1998, title={Expansion and Length-Dependent Fragility of CTG Repeats in Yeast}, volume={279}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.279.5352.853}, DOI={10.1126/science.279.5352.853}, number={5352}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Freudenreich, Catherine H. and Kantrow, Sara M. and Zakian, Virginia A.}, year={1998}, month=feb, pages={853–856} }