Role of Vesicle-Associated Syntaxin 5 in the Assembly of Pre-Golgi Intermediates
10.1126/science.279.5351.696
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Syntaxins are thought to function during vesicular transport as receptors on the target membrane and to contribute to the specificity of membrane docking and fusion by interacting with vesicle-associated receptors. Here, syntaxin 5 (Syn5) was shown to be an integral component of endoplasmic reticulum–derived transport vesicles. This pool, but not the target, Golgi-associated Syn5 pool, was essential for the assembly of vesicular-tubular pre-Golgi intermediates and the delivery of cargo to the Golgi. The requirement for vesicle-associated Syn5 in transport suggests a reevaluation of the basis for operation of the early secretory pathway.

Bibliography

Rowe, T., Dascher, C., Bannykh, S., Plutner, H., & Balch, W. E. (1998). Role of Vesicle-Associated Syntaxin 5 in the Assembly of Pre-Golgi Intermediates. Science, 279(5351), 696–700.

Authors 5
  1. Tony Rowe (first)
  2. Christiane Dascher (additional)
  3. Sergei Bannykh (additional)
  4. Helen Plutner (additional)
  5. William E. Balch (additional)
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  48. We thank R. Scheller and J. Hay (Stanford University) for antibodies to mSec22b rBet1 and membrin; J. Saraste (University of Bergen Bergen Norway) for anti-p58; and S. Whiteheart (University of Kentucky Medical Center) for recombinant α-SNAP Myc-tagged NSF and anti-NSF (6E6). Supported by NIH grant GM 42336 (to W.E.B.) and the National Cancer Institute (CA58689). Electron microscopy made extensive use of Core B in CA58689. T.R. is a recipient of a fellowship from the Muscular Dystrophy Association C.D. is a recipient of a fellowship grant from the Deutsche Forschungsgemeinschaft and S.B. is a recipient of a Cystic Fibrosis Foundation Fellowship.
Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:49 a.m.)
Deposited 1 year, 7 months ago (Jan. 13, 2024, 12:57 a.m.)
Indexed 1 month, 1 week ago (July 19, 2025, 11:36 p.m.)
Issued 27 years, 7 months ago (Jan. 30, 1998)
Published 27 years, 7 months ago (Jan. 30, 1998)
Published Print 27 years, 7 months ago (Jan. 30, 1998)
Funders 0

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@article{Rowe_1998, title={Role of Vesicle-Associated Syntaxin 5 in the Assembly of Pre-Golgi Intermediates}, volume={279}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.279.5351.696}, DOI={10.1126/science.279.5351.696}, number={5351}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Rowe, Tony and Dascher, Christiane and Bannykh, Sergei and Plutner, Helen and Balch, William E.}, year={1998}, month=jan, pages={696–700} }