Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts
10.1126/science.278.5346.2130
Crossref journal-article
American Association for the Advancement of Science (AAAS)
Science (221)
Abstract

Ovine primary fetal fibroblasts were cotransfected with a neomycin resistance marker gene ( neo ) and a human coagulation factor IX genomic construct designed for expression of the encoded protein in sheep milk. Two cloned transfectants and a population of neomycin (G418)–resistant cells were used as donors for nuclear transfer to enucleated oocytes. Six transgenic lambs were liveborn: Three produced from cloned cells contained factor IX and neo transgenes, whereas three produced from the uncloned population contained the marker gene only. Somatic cells can therefore be subjected to genetic manipulation in vitro and produce viable animals by nuclear transfer. Production of transgenic sheep by nuclear transfer requires fewer than half the animals needed for pronuclear microinjection.

Bibliography

Schnieke, A. E., Kind, A. J., Ritchie, W. A., Mycock, K., Scott, A. R., Ritchie, M., Wilmut, I., Colman, A., & Campbell, K. H. S. (1997). Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts. Science, 278(5346), 2130–2133.

Authors 9
  1. Angelika E. Schnieke (first)
  2. Alexander J. Kind (additional)
  3. William A. Ritchie (additional)
  4. Karen Mycock (additional)
  5. Angela R. Scott (additional)
  6. Marjorie Ritchie (additional)
  7. Ian Wilmut (additional)
  8. Alan Colman (additional)
  9. Keith H. S. Campbell (additional)
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  37. We thank J. Bracken and M. Malcolm-Smith for technical assistance in large animal work H. Bowran and D. McGavin for care of the animals D. Cottom for providing data on microinjection studies the Animal Sciences Team at PPL Therapeutics for help in deriving the PDFF cells Y. Gibson and the Small Animal Unit at PPL Therapeutics for generating FIX transgenic mice S. Bruce for FIX protein analysis G. G. Brownlee for human FIX genomic λ clones D. Melton for PGKneo A. R. Scott for technical assistance with molecular biology and I. Garner for constructive discussions during the course of the project. The experiments were conducted under the Animals (Scientific Procedures) Act 1986 and with the approval of the Roslin Institute Animal Welfare and Experiments Committee.
Dates
Type When
Created 23 years, 1 month ago (July 27, 2002, 5:45 a.m.)
Deposited 1 year, 7 months ago (Jan. 12, 2024, 11:52 p.m.)
Indexed 3 weeks, 4 days ago (Aug. 7, 2025, 4:25 p.m.)
Issued 27 years, 8 months ago (Dec. 19, 1997)
Published 27 years, 8 months ago (Dec. 19, 1997)
Published Print 27 years, 8 months ago (Dec. 19, 1997)
Funders 0

None

@article{Schnieke_1997, title={Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts}, volume={278}, ISSN={1095-9203}, url={http://dx.doi.org/10.1126/science.278.5346.2130}, DOI={10.1126/science.278.5346.2130}, number={5346}, journal={Science}, publisher={American Association for the Advancement of Science (AAAS)}, author={Schnieke, Angelika E. and Kind, Alexander J. and Ritchie, William A. and Mycock, Karen and Scott, Angela R. and Ritchie, Marjorie and Wilmut, Ian and Colman, Alan and Campbell, Keith H. S.}, year={1997}, month=dec, pages={2130–2133} }